The Effect of Solvent Hydrophilicity on the Enzymatic Ring-Opening Polymerization of L-Lactide by Candida rugosa Lipase.

Contradictions have been reported on the effect of organic solvents, especially toluene, on enzymatic ring-opening polymerization (eROP) of L-lactide. Studies have shown that log P, a common measure of hydrophilicity, affects enzyme activity. This study examines the effect of solvents with various log P values on the eROP of L-lactide, performed using Candida rugosa lipase (CRL). N,N-dimethylacetamide (DMA), 1,2-dimethoxybenzene, 1,4-dimethoxybenzene, diphenyl ether, and dodecane were used as the organic solvents. The eROP in ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]) was also conducted to compare its performance with the organic solvents. The results show that [BMIM][PF6]-mediated eROP gave better conversion and molecular weight than the organic solvent-mediated eROP. In this study, the effects of solvents hydrophilicity are discussed, including the possibility of hexafluorophosphate ion ([PF6]-) hydrolysis to occur.

Sequencing Batch Integrated Fixed-Film Activated Sludge Membrane Process for Treatment of Tapioca Processing Wastewater.

Tapioca processing industries are very popular in the rural community to produce a variety of foods as the end products. Due to their small scales and scattered locations, they require robust modular systems to operate at low capacity with minimum supervision. This study explores the application of a novel sequencing batch-integrated fixed-film activated sludge membrane (SB-IFASM) process to treat tapioca processing wastewater for reuse purposes. The SB-IFASM employed a gravity-driven system and utilizes biofilm to enhance biodegradation without requiring membrane cleaning. The SB-IFASM utilizes the biofilm as a secondary biodegradation stage to enhance the permeate quality applicable for reuse. A lab-scale SB-IFASM was developed, preliminarily assessed, and used to treat synthetic tapioca processing industry wastewater. The results of short-term filtration tests showed the significant impact of hydrostatic pressure on membrane compaction and instant cake layer formation. Increasing the pressure from 2.2 to 10 kPa lowered the permeability of clean water and activated sludge from 720 to 425 and from 110 to 50 L/m2·h bar, respectively. The unsteady-state operation of the SB-IFASM showed the prominent role of the bio-cake in removing the organics reaching the permeate quality suitable for reuse. High COD removals of 63-98% demonstrated the prominence contribution of the biofilm in enhancing biological performance and ultimate COD removals of >93% make it very attractive for application in small-scale tapioca processing industries. However, the biological ecosystem was unstable, as shown by foaming that deteriorated permeability and was detrimental to the organic removal. Further developments are still required, particularly to address the biological stability and low permeability.

Enrichment of thermophilic methanogenic microflora from mesophilic waste activated sludge for anaerobic digestion of garbage slurry.

This study investigated a startup strategy for thermophilic methanogenic enrichment. Conventional waste activated sludge (WAS) was used as the seed. The WAS seed was incubated at 55 °C in a continuous-flow stirred tank reactor, with garbage slurry fed continuously as a substrate. One of the two reactors (termed reactor-high, RH) was fed with a high concentration of substrate (30 g-COD/L), while the other (reactor-low, RL) received a lower concentration of feed (15 g-COD/L). The specific organic loading rate was 0.2 g-COD/L/day initially, which was gradually increased by shortening the hydraulic retention time. The final OLR was 3.2 g-COD/L/day, after more than 90% of the initial WAS got washed out from the reactor and thermophilic microorganisms became dominant in the reactors. Biogas production rate and methane conversion ratio depended on substrate concentration, although total chemical oxygen demand removal and methane content were almost the same in RH and RL. Biogas production rate in RH was 3.2 times higher than that in RL, while the conversion ratio of RH was 1.6 times higher than that of RL. Quantitative polymerase chain reaction analysis using specific primers for the mcrA gene and high-throughput sequencing analysis of 16S rRNA gene amplicons demonstrated post enrichment differences in the microbial community, relative to that in the WAS. There was no significant difference in the enriched microbial community composition between RH and RL. In conclusion, thermophilic methanogenic microflora can be enriched from mesophilic seeds. Methanothermobacter, Methanosarcina, and other thermophilic bacteria were enriched in the community over time, with these thermophiles collectively accounting for ∼80% of the stable thermophilic community.