[Genoprotective effect of drugs based on ecdysteroids in poisoning of rats with tetrachloromethane and chlorophos]. / Genoprotektornyi éffekt preparatov na osnove ékdisteroidov pri otravlenii krys tetrakhlormetanom i khlorofosom.

Molecular mechanisms of chromatin damage have been investigated during tetrachloromethane and chlorophos intoxication of experimental animals. Introduction of tetrachloromethane to experimental animals induced chromatin degradation causing a partial loss of histone H1-DNA fragmentation and formation of intermolecular bonds: DNA-protein. Intoxication with chlorophos results in repression of a part of genes due to augmented chromatin compactness. Preventive introduction of the steroid preparation from Serratula coronata L. to experimental animals exerts a genoprotective effect, probably, as a result of derepression of genes responsible for reparation of chromatin structure.

[The use of the multicopy plasmid pUC19 for assuring the constitutive expression of gene rplL in Escherichia coli]. / Ispol'zovanie mnogokopiinoi plazmidy pUC19 dlia obespecheniia konstitutivnoi ékspressii gena rplL Escherichia coli.

A mutation in lac-operator region of pUC19 plasmid causing an increase in beta-galactosidase activity was observed. The plasmid was used as a vector to provide high level of expression of the cloned E. coli rplL gene.