Establishment and multi drug resistance evolution of ST235 Pseudomonas aeruginosa strains in the intensive care unit of a Colombian hospital.

Drug resistant Pseudomonas aeruginosa represents a therapeutic challenge. To assess the diversity of P. aeruginosa antibiotic resistant variants, isolates were recovered from hospital patients in Colombia. Thirty of 60 isolates contained class 1 integrons and five were of Sequence Type ST235 having appeared in a single intensive care unit. All five possessed an unusual integron but showed differences in gene cassette content and the presence/absence of insertion sequence IS26. This showed that differences can arise rapidly, even within a single ICU. Also, the emergence of IS26 in P. aeruginosa is contributing to the evolution of resistance in this bacterium.

[A high prevalence of antibodies against Bartonella and Babesia microti has been found in villages and urban populations in Cordoba, Colombia]. / Alta Prevalencia de Anticuerpos contra Bartonella y Babesia microti en Poblaciones Rurales y Urbanas en dos Provincias de Córdoba, Colombia.

OBJECTIVE: Establishing seroprevalence against Bartonella and Babesia microti in arural population exposed and/or non-exposed to domestic animals in Cordoba,Colombia. METHODOLOGY: Sera samples taken from 80 people from Montería and Cereté (Córdoba department) were analysed; the population sample was chosen by non-probabilistic means. Anti-Bartonella and Babesia microti were detected by indirect immunofluorescence (IFI), (Focus Technologies, Cypres, CA, USA). RESULTS: Total anti-bartonella IgG seroprevalence was 48,7 % (39/80), 77 % being male and 23 % female. Bartonella quintana seropositivity was 45 % (36/80) and Bartonella henselae seropositivity 30 % (24/80); 21 of these individuals (26,2 %) had antibodies to both bartonellas. Babesia microti seroprevelence was 30,6 % (23/80),65 % being male patients. CONCLUSIONS: High Bartonella and Babesia seroprevelence showed that infection levels had been underestimated in Colombia. Medical and sanitary authorities on the Caribbean coast of Colombia must take measures for monitoring the distribution,propagation and identification of human populations at risk of contracting infection by these micro-organisms and also orientate diagnosis and enable suitable prevention strategies to be developed for these diseases.