RESUMO
Protease-encoding nucleotide sequences of 27 HIV-1 variants isolated in Russia and other CIS countries from seropositive intravenous drug-users were analyzed. None of the above persons did ever take antiretroviral drugs. The nucleotide sequences were shown to belong to subtypes A and to be have a high degree of genetic homogeneity (0.00-3.23; mean--1.38 +/- 0.79). No isolates contained any primary mutations of resistance to protease inhibitors. At the same time, above one half of the isolates bore the V771 substitution, which, according to published data, is the secondary mutation of resistance that conditions a higher resistance to Nelfinavir. Moreover, the substitution was associated with 2 synonymous mutations in triplets 31 and 78, which denotes a single origin for all V771 variants.
Assuntos
Protease de HIV/genética , Soropositividade para HIV/epidemiologia , HIV-1/genética , Abuso de Substâncias por Via Intravenosa/epidemiologia , Adolescente , Adulto , Sequência de Aminoácidos , HIV-1/isolamento & purificação , Humanos , Epidemiologia Molecular , Dados de Sequência Molecular , Mutação , Filogenia , RNA Viral/genética , Federação Russa/epidemiologia , Alinhamento de Sequência , Ucrânia/epidemiologiaRESUMO
A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).