Ultra-high-performance liquid chromatography-tandem high-resolution elevated mass spectrometry profiling of anti-methicillin-resistant Staphylococcus aureus metabolites from the endophytic bacteria collected from the weeds of a previous dumpsite.

The culturable endophytic bacteria from the weeds Cleome rutidosperma of the family Cleomaceae and Digitaria sanguinalis of the family Poaceae obtained from a previous dumpsite in Pampanga, Philippines have been assessed for their anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and the analytes with such activity should be identified. However, due to the limited amounts collected from the isolation process, 1.8 mg yield of compound 1 from the endophyte of C. rutidosperma and 1.2 mg of a mixture from the endophyte of D. sanguinalis were selected for LC-MSE analysis. The production of compounds from the culturable endophytic bacteria Pseudomonas aeruginosa- determined by gene-sequencing, an untargeted and data-independent analysis (DIA) by ultra-high performance liquid chromatography-high resolution-elevated energy mass spectrometry (UHPLC-HR-MSE) technique was employed to profile the metabolites present in the two high-performance liquid chromatography (HPLC) fractions. The analytes present from P. aeruginosa detected by UHPLC-HR-MSE isolated from C. rutidosperma was phenazine-1-carboxylic acid (1), and for D. sanguinalis were chamigrenal (2), dialkyl resorcinol (3), and a pyoverdine elicitor (4). This study proves that UHPLC-HR-MSE could identify the anti-MRSA constituents in P. aeruginosa from commensal weeds C. rutidosperma and D. sanguinalis. The UHPLC-HR-MSE could help strengthen metabolomics antibacterial research and its related applications from a future perspective. Application of metabolomics research using UHPLC-HR-MSE could enhance the rehabilitation of dumpsites by the microbial community present.

Achieving accurate laboratory diagnosis of typhoid fever: a review and meta-analysis of TUBEX® TF clinical performance.

This review discusses currently available serological diagnostic methods for typhoid fever with a focus on the clinical utility of TUBEX® TF as an alternative to the Widal or Typhidot test. A literature search was conducted in PubMed for related publications written in English. A qualitative analysis was done to determine various serological tests used for typhoid fever diagnosis with emphasis on TUBEX® TF in comparison to the Widal of Typhidot test. Further, a meta-analysis was performed to obtain a pooled estimate of diagnostic accuracy (sensitivity and specificity) using different analysis models. A total of sixteen studies was included in the qualitative analysis. Further screening of these studies yielded ten studies that were used for the meta-analysis. The sensitivity/specificity range of different commonly used serological tests in typhoid patients is between 55-100%/58-100% for TUBEX® TF, 54-67%/54-95% for Typhidot, and 32-95%/4-98% for the Widal test. As for the pooled meta-analysis estimates, the TUBEX® TF showed superior results when differentiating individuals with febrile illness of unknown origin from those with typhoid fever. Overall, the results of this review and meta-analysis suggest that the TUBEX® TF is more advantageous to use as a serological test for typhoid fever diagnosis due its accuracy and simplicity. However, further studies are still needed to validate our results.

Reducing the incidence of pregnancy-related urinary tract infection by improving the knowledge and preventive practices of pregnant women.

OBJECTIVE: Pregnancy-related urinary tract infections (UTI) is the leading cause of obstetrical ward admissions and is responsible for poor maternal and perinatal outcomes. This study aimed to reduce the incidence of UTI by improving the knowledge and preventive practices of pregnant women through the implementation of a health education package. STUDY DESIGN: A health education package consisting of a seminar, sending of weekly text messages, and distribution of educational leaflets on UTI awareness and prevention was implemented in various rural health units in Pampanga, Philippines. A structured questionnaire was used to assess the pre- and post-intervention knowledge and preventive practices of pregnant women. Whereas, urinalysis results from the various rural health units were used to assess the incidence of UTI among the respondents. RESULTS: Significant improvement (p <  0.001) was observed regarding the participant's knowledge and water intake after the intervention. Although there was no significant change (p =  0.16) in their hygiene statistically, all participants had improvements in hygiene practices after the intervention. The number of pregnant women who were positive for UTI also decreased significantly (p <  0.001) following the intervention. CONCLUSION: The implemented health education package was able to reduce the incidence of pregnancy-related UTI by improving the knowledge and preventive practices of pregnant women.

Pet owners' awareness on RA 9482 (Anti-Rabies Act of 2007) in Magalang, Pampanga Philippines.

BACKGROUND: Rabies remains a significant public health problem in the Philippines. An acutely fatal viral infection, it results in the death of 200-300 Filipinos annually affecting mostly children less than 15 years old. The disease is endemic in Magalang which ranks fifth in the number of dog rabies positive cases among the 19 municipalities of Pampanga. In spite of the enactment of the Anti-Rabies Act of 2007 (RA 9482), the control of rabies in Magalang has been a major challenge for the municipality. Thus, this study investigated the awareness of pet owners in Magalang, Pampanga on the Anti-Rabies Act of 2007 (RA 9482). METHODS: This study was a cross-sectional study conducted from April-September 2017 among 380 pet owners from eight urban and rural barangays in Magalang, Pampanga. Data gathered were analyzed using STATA IC (Ver13.0); Bloom's cut-off points were used to categorize the level of knowledge of pet owners on the Anti-Rabies Act; data were further analyzed using Chi-square Test and relationship between variables was determined through logistic regression. RESULTS: Results revealed that pet owners in Magalang had poor knowledge of the Anti-Rabies Act (65.5%), and majority (71.3%) were unaware of its existence and of penalties imposed by the law to non-compliant pet owners. Moreover, results indicate that pet owners in urban places have higher odds of having above median knowledge on the law compared to those in rural places.

Magneto-actuated immunoassay for the detection of Mycobacterium fortuitum in hemodialysis water.

This paper addresses a sensitive method for the detection of mycobacteria in hemodialysis water samples based on a magneto-actuated immunoassay with optical readout. In this approach, micro (2.8µm) sized magnetic particles were modified with an antibody against the lipoarabinomannan (LAM) located in the mycobacterial cell wall. The system relies on the immunocapturing of the mycobacteria with the tailored antiLAM magnetic particles to pre-concentrate the bacteria from the hemodialysis samples throughout an immunological reaction. The performance of the immunomagnetic separation on the magnetic carrier was evaluated using confocal microscopy to study the binding pattern, as well as a magneto-actuated immunoassay with optical readout for the rapid detection of the bacteria in spiked hemodialysis samples. In this approach, the antiLAM polyclonal antibody was labeled with fluorescein isothiocyanate. The optical readout was achieved by the incubation with a secondary anti-fluorescein antibody labeled with peroxidase as optical reporter. The magneto-actuated immunoassay was able to detect mycobacteria contamination in hemodialysis water at a limit of detection of 13CFUmL(-1) in a total assay time of 3h without any previous culturing pre-enrichment step.