FISH examination of lymphocytes from Mayak workers for assessment of translocation induction rate under chronic radiation exposures.

PURPOSE: To estimate the translocation-induction rate under chronic exposure conditions by measuring chromosome aberration frequencies in lymphocytes from Mayak nuclear workers using fluorescence in situ hybridization (FISH). MATERIALS AND METHODS: Lymphocytes were examined from 27 nuclear workers at the Mayak Production Association and two control individuals using FISH with probes for chromosomes 1, 2 and 4. Official doses derived from worker film-badge records varied from 0 to 8.50 Gy. RESULTS: The mean (+/-SD) genome-equivalent translocation frequency (F(G)) was 2.30 (+/-0.75)% in the zero-dose group (n = 7), and Poisson regression analysis provided the best-fit equation of F(G)(%) = 2.96(+/-0.39) + 0.69(+/-0.14)D + 0.12(+/-0.05)A, where D is the film-badge-derived dose (Gy), and A is age centred at 67 years. The induction rate would increase to nearly 1% Gy(-1) if the radiation dose to bone marrow, one of the major organs for lymphocytes and where their precursor cells reside, is considered. CONCLUSION: The estimated induction rate in vivo appeared substantially smaller than linear coefficients estimated from various in vitro studies.

Biomarkers specific to densely-ionising (high LET) radiations.

There have been several suggestions of biomarkers that are specific to high LET radiation. Such a biomarker could significantly increase the power of epidemiological studies of individuals exposed to densely-ionising radiations such as alpha particles (e.g. radon, plutonium workers, individuals exposed to depleted uranium) or neutrons (e.g. radiation workers, airline personnel. We discuss here a potentially powerful high LET biomarker (the H value) which is the ratio of induced inter-chromosomal aberrations to intra-arm aberrations. Both theoretical and experimental studies have suggested that this ratio should differ by a factor of about three between high LET radiation and any other likely clastogen, and will yield more discrimination than the previously suggested F value (ratio of inter-chromosomal aberrations to intra-chromosomal inter-arm aberrations). Evidence of the long-term stability of such chromosomal biomarkers has also been generated. Because these stable intra-arm anld inter-chromosomal aberrations are (1) frequent and (2) measurable at long times after exposure, this H value appears to be a practical biomarker of high LET exposure, and several in vitro studies have confirmed the approach for unstable aberrations. The approach is currently being tested in a population of Russian radiation workers exposed several decades ago to high- or low LET radiation.

College students' use of widely advertised medications.

The authors describe college and university students' use of widely advertised pharmaceutical products. Four hundred seventy-one students from three institutions completed self-administered questionnaires that addressed the use of advertised medications, attention to magazine ads for medications, communications with physicians about medications, and the conditions the medications address. Results indicated that the majority of the students used at least one of the advertised products. Most students did not discuss the pharmaceutical products with their physicians or discuss the conditions for which they reported they were taking the drugs.

Effects of direct-to-consumer advertising of pharmaceutical products on college students.

This study examines college and university students' responses to direct-to-consumer advertising of pharmaceutical products in magazines. Four hundred seventy-one students from three institutions participated in the study that found that the majority of the students were frequent magazine readers, they noticed ads for the pharmaceutical products, and they used the advertised products. Although most students denied buying or using the products because of the ads, there was a significant correlation between number of ads seen and number of products used.

Using the Health Belief Model to examine and predict college women's cervical cancer screening beliefs and behavior.

The high prevalence of human papillomavirus (HPV) among adolescent and young adult women and the causal association between certain types of HPV and cervical cancer make regular gynecological screening and Pap smear testing essential health practices for young women. In this study, we used the constructs of the Health Belief Model (HBM) to examine the gynecological screening beliefs and behaviors of a sample of 400 college women. Although the constructs of the model were able to explain only 15% of the variance in screening behavior and 11% of screening intentions, the use of the HBM framework resulted in important information regarding the participants' beliefs.

Examination and prediction of elementary school teachers' intentions to teach HIV/AIDS education.

Although studies have looked at school teachers' beliefs and attitudes toward AIDS and students with HIV/AIDS, none have specifically addressed teachers' intentions and attitudes toward teaching HIV/AIDS education. This study examines and predicts elementary school teachers' intentions to teach their students about HIV/AIDS. The postulates of the theories of reasoned action and planned behavior provided the framework for the examination of teachers' beliefs, attitudes, subjective norms, and perceived behavioral control regarding AIDS education. A sample of 198 elementary school teachers employed in the Commonwealth of Massachusetts completed self-administered questionnaires. Multiple regression analysis was used to predict intentions to teach HIV/AIDS, and to identify the determinants of intentions. Attitude, subjective norm, and perceived behavioral control, the variables of the theory of planned behavior, explained 64% of the variance in teachers' intentions; perceived behavioral control contributed the greatest weight to the prediction. Significant variance was additionally explained by three variables external to the theory of planned behavior: in-service training, grade taught, and past HIV/AIDS teaching behavior.