Assuntos
Encefalopatias/microbiologia , Febre das Trincheiras/diagnóstico , Adulto , Bartonella quintana/genética , Bartonella quintana/isolamento & purificação , Sequência de Bases , Encefalopatias/líquido cefalorraquidiano , Encefalopatias/diagnóstico , Edema Encefálico/etiologia , Criança , Citrato (si)-Sintase/genética , Epilepsia Tônico-Clônica/etiologia , Granuloma/diagnóstico , Granuloma/microbiologia , Cefaleia/etiologia , Hemiplegia/etiologia , Humanos , Masculino , Reação em Cadeia da Polimerase , Homologia de Sequência do Ácido Nucleico , Distúrbios da Fala/etiologia , Doenças Talâmicas/diagnóstico , Doenças Talâmicas/microbiologia , Febre das Trincheiras/líquido cefalorraquidianoRESUMO
Maternal immunization might protect infants from severe disease due to respiratory syncytial virus (RSV). Guinea pigs are susceptible to infections with RSV and transfer antibodies to their offspring prenatally. Pregnant guinea pigs were immunized by infection with RSV and their offspring were challenged intranasally with RSV. Pulmonary viral replication was compared among the pups born to immunized mothers (group A) and the pups from nonimmune mothers (group B) in two studies. Mean (+/-SD) log10 virus titers were, in study 1, group A, 2.3 +/- 0.8 pfu/g of lung (n = 10); group B, 3.6 +/- 1.5 pfu/g (n = 13) (P = .0058); and study 2, group A, < 1.69 pfu/g (n = 8); group B, 3.4 +/- 0.9 pfu/g (n = 6) (P = .0002). Thus, immunization of pregnant guinea pigs resulted in a significant reduction in viral replication in the lungs of their offspring. Guinea pigs should be useful for the study of maternal immunization against RSV.
Assuntos
Imunidade Materno-Adquirida , Infecções por Vírus Respiratório Sincicial/prevenção & controle , Vírus Sinciciais Respiratórios/imunologia , Animais , Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Cobaias , Imunização , Pulmão/patologia , Pulmão/virologia , Gravidez , Replicação ViralRESUMO
The ability to measure antibodies against individual respiratory syncytial virus (RSV) proteins is important in the analysis of immune responses to RSV. We expressed the nucleocapsid (N) protein and the group A and B RSV attachment (G) proteins from recombinant baculoviruses. The three recombinant RSV proteins were used individually in an enzyme-linked immunosorbent assay (ELISA; bac-ELISA for results from assays of all three proteins). The bac-ELISA results were compared to the results obtained by a whole-virus ELISA (RS-ELISA for results from assays of both group A and B viruses). Antibody samples from 113 children were tested. The determination of seronegative or seropositive status by the bac-ELISA was compared to the same determination by the RS-ELISA; the sensitivity of bac-ELISA was 87% (95% confidence interval [CI], 78 to 93%), the specificity was 82% (CI, 59 to 94%), and the positive and negative predictive values were 95% (CI, 86 to 98%) and 60% (CI, 41 to 77%), respectively. The group specificity of the G-protein ELISA was confirmed by testing antibodies from experimentally immunized animals. Thus, the bac-ELISA was shown to be comparable to the whole-virus ELISA in detecting antibody responses to RSV, while it offered the advantage of measuring specific antibody responses to individual RSV proteins.
