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1.
Toxicol Appl Pharmacol ; 165(2): 148-57, 2000 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-10828210

RESUMO

In this study, the mouse was used to evaluate paternal germline exposure to the organophosphate methamidophos for its potential to produce adverse effects on spermatozoa and in the offspring. There have been reports that organophosphate exposure can increase abnormal sperm morphology in mice. However, effects transmitted to the offspring following paternal exposure have not been reported previously. The maximum tolerated dose (MTD) was 7.5 mg kg(-1) body weight and this dose resulted in no deaths, although blood plasma cholinesterase activity was still decreased. Males were euthanized 4 weeks after an acute intraperitoneal injection of methamidophos (0.5, 3.75, 5.0, and 7.5 mg kg(-1) body wt) and the number of spermatids per gram testes and sperm morphology were analyzed. In this study, abnormal sperm morphology on a per group basis exhibited a dose-response significantly related to increased methamidophos exposure as indicated by regression analysis and a nested ANOVA (p < 0.0001). Preimplantation embryos that were conceived 6 weeks after paternal methamidophos exposure (5 mg kg(-1) body wt) exhibited a significant increase in cleavage arrest. Fertility of males was also affected as shown by a decrease in the number of two- to four-cell embryos per male (postexposure week 6) and an increase in the number of degenerated embryos (postexposure weeks 4-6). We conclude that methamidophos may have the potential to produce transmissible adverse embryonic effects following an acute paternal germline exposure.


Assuntos
Inseticidas/toxicidade , Compostos Organotiofosforados/toxicidade , Espermatozoides/efeitos dos fármacos , Acetilcolinesterase/sangue , Animais , Blastocisto/efeitos dos fármacos , Contagem de Células , Divisão Celular/efeitos dos fármacos , Desenvolvimento Embrionário e Fetal/efeitos dos fármacos , Feminino , Fertilização/efeitos dos fármacos , Inseticidas/sangue , Masculino , Camundongos , Compostos Organotiofosforados/sangue , Contagem de Espermatozoides/efeitos dos fármacos , Espermátides/efeitos dos fármacos , Espermatozoides/ultraestrutura
2.
Mutat Res ; 381(1): 59-66, 1997 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-9403031

RESUMO

Previous studies suggest that the spermatozoa from acutely irradiated male mice exhibit a reduced fertilization rate in vitro with the maximum decrease occurring for spermatozoa produced 6 weeks after irradiation (Y. Matsuda et al., Mutation Res. 142 (1985) 59-63). We have found that spermatozoa from unirradiated F1 males conceived 6 weeks after paternal F0 irradiation also exhibit a significantly reduced fertilization rate in vitro. After acute 137Cs gamma-irradiation yielding an absorbed dose of 1.0 Gy, adult CD1 F0 male mice were mated at weekly intervals with unirradiated female CD1 mice. Unirradiated adult males from F1 litters conceived 5 and 6 weeks after paternal F0 irradiation were allowed to mature. Their epididymal spermatozoa were evaluated for in vitro fertilization rates using oocytes from unirradiated 8-12-week-old CD1 females. The mean fertilization rate for spermatozoa from F1 males conceived 5 weeks after paternal F0 irradiation (80.74 +/- 15.74 SD %, n = 5) did not differ significantly from the control fertilization rate (89.40 +/- 10.94 SD %, n = 8). However, the fertilization rate for spermatozoa from F1 males conceived 6 weeks after paternal F0 irradiation (56.14 +/- 21.93 SD %, n = 5) was significantly less than the fertilization rate for control spermatozoa (p < 0.006) or for that of the F1 males conceived 5 weeks after paternal F0 irradiation (p < 0.04). These data suggest that spermatozoa obtained 6 weeks after paternal F0 irradiation can transmit a decrease in fertilization rate to the F1 generation males as well as exhibit decreased fertilization rate themselves when tested directly in vitro.


Assuntos
Fertilização in vitro/efeitos da radiação , Espermatozoides/fisiologia , Espermatozoides/efeitos da radiação , Animais , Relação Dose-Resposta à Radiação , Embrião de Mamíferos/fisiologia , Feminino , Fertilidade , Raios gama , Masculino , Camundongos , Camundongos Endogâmicos , Oócitos/fisiologia , Irradiação Corporal Total
3.
Biol Reprod ; 55(3): 709-14, 1996 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-8862791

RESUMO

Because in the mouse some structurally abnormal spermatozoa can penetrate oocytes, we decided to determine whether mouse oocytes fertilized by spermatozoa with grossly misshapen heads are able to develop normally. We injected such spermatozoa from BALB/c mice individually into mature oocytes of hybrid mice B6D2F1. Eighty to ninety percent of the oocytes were activated and developed into blastocysts in vitro and, when transferred to foster mothers, some developed into apparently normal fertile adults. This finding indicates that a proportion of abnormal spermatozoa carry all the genome and organelles necessary for normal embryonic development and growth to fertile maturity.


Assuntos
Desenvolvimento Embrionário e Fetal/fisiologia , Oócitos/fisiologia , Cabeça do Espermatozoide/fisiologia , Espermatozoides/fisiologia , Animais , Meios de Cultura , Desenvolvimento Embrionário e Fetal/efeitos dos fármacos , Epididimo/citologia , Epididimo/efeitos dos fármacos , Feminino , Indicadores e Reagentes , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica , Gravidez , Cabeça do Espermatozoide/ultraestrutura , Testículo/citologia
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