RESUMO
The biological activity of lipids and photosynthetic pigments of the kelp Sargassum pallidum (Turner) C. Agardh has been studied. Free fatty acids and their esters demonstrated considerable antimicrobial activity against bacteria (Staphylococcus aureus[ital] and Escherichia coli), yeast-like fungi (Candida albicans), and opportunistic pathogenic (Aspergilius niger) and phytopathogenic (Fusarium oxysporum, and Septoria glycines) fungi. Glyceroglycolipids and neutral lipids demonstrated moderate activity. Fucoxanthin and chlorophylls weakly suppressed the growth of microorganisms. None of the studied substances demonstrated activity against Ehrlich's carcinoma. It was shown that the season of weed harvesting affected both antimicrobial and hemolytic activities of different lipids due to changes in their fatty acid composition.
Assuntos
Anti-Infecciosos/farmacologia , Ácidos Graxos não Esterificados/farmacologia , Ácidos Graxos/farmacologia , Pigmentos Biológicos/farmacologia , Sargassum/química , Animais , Anti-Infecciosos/isolamento & purificação , Candida albicans/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Carcinoma de Ehrlich/patologia , Sobrevivência Celular/efeitos dos fármacos , Clorofila/isolamento & purificação , Clorofila/farmacologia , Eritrócitos/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Ácidos Graxos/isolamento & purificação , Ácidos Graxos não Esterificados/isolamento & purificação , Fusarium/efeitos dos fármacos , Fusarium/crescimento & desenvolvimento , Glicolipídeos/isolamento & purificação , Glicolipídeos/farmacologia , Camundongos , Fotossíntese/fisiologia , Pigmentos Biológicos/isolamento & purificação , Estações do Ano , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento , Células Tumorais Cultivadas , Xantofilas/isolamento & purificação , Xantofilas/farmacologiaRESUMO
Antimicrobial and hemolytic activity of ethanol extract of brown seaweed Laminaria cichorioides Miyabe, its lipophilic fractions, various classes of substances of lipophilic fraction, such as chlorophylls, fucoxanthin, monogalactosyldiacylglycerols, digalactosyldiacylglycerols, sulfoquinovosyldiacylglycerols, and fatty acids were investigated. The antimicrobial activity was studied by means of yeast cells Safale S-04 and Candida albicans CMM 455, fungi Aspergillus niger CMM 4634 and Fusarium oxysporum CMM 4639, bacteria Staphylococcus aureus ATCC 21027 (gram-positive) and Escherichia coli ATCC 15034 (gram-negative) which demonstrated selective sensitivity to the studied substances. Hemolytic activity was investigated at concentrations of substances in the range of 0.2-2000 microg/ml at different pH of erythrocyte suspension. All investigated substances caused hemolysis. The dependence of hemolytic activity of substances on pH of media was determined.
Assuntos
Anti-Infecciosos/farmacologia , Hemolíticos/farmacologia , Laminaria/química , Animais , Anti-Infecciosos/isolamento & purificação , Aspergillus niger/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Fusarium/efeitos dos fármacos , Hemólise , Hemolíticos/isolamento & purificação , Camundongos , Staphylococcus aureus/efeitos dos fármacosRESUMO
The amount and composition of fatty acids in the fungus Stilbella aciculosa associated with the marine macroorganism Apostichopus japonica (trepang) were determined by gas-liquid chromatography and gas chromatography-mass spectrometry. In the culture liquid of S. aciculosa, prostaglandins (PG) of groups E and F were revealed by UV spectroscopy. This finding was confirmed by the presence of direct precursors of PG, polyunsaturated eicosapentaenoic and docosahexaenoic acids, in the culture liquid. The biomass of this fungus contained PG of group B.
Assuntos
Ascomicetos/metabolismo , Prostaglandinas B/biossíntese , Prostaglandinas E/biossíntese , Prostaglandinas F/biossíntese , Animais , Ascomicetos/crescimento & desenvolvimento , Ascomicetos/isolamento & purificação , Cromatografia Gasosa , Meios de Cultivo Condicionados/metabolismo , Ácidos Docosa-Hexaenoicos/análise , Ácidos Docosa-Hexaenoicos/metabolismo , Ácido Eicosapentaenoico/análise , Ácido Eicosapentaenoico/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Prostaglandinas B/isolamento & purificação , Prostaglandinas E/isolamento & purificação , Prostaglandinas F/isolamento & purificação , Stichopus/microbiologiaRESUMO
1. The alteration of lipid composition has been shown to take place at different stages of antler growth. 2. The greatest amounts of phospholipids and polyunsaturated fatty acids have been found during the most intense soft antler growth period. 3. The bioregulators of lipid origin which are prostaglandins of A, B, E and F groups have been found at the same stage.
