RESUMO
By investigating a group of 67 unrelated adult persons suffering from vitiligo it was found that antigens HLA-A2 and HLA-Dw7 differed in their frequencies in comparison to those observed in the healthy population. The antigen HLA-A2 was found in 76.12% of patients compared to 43.95% in the control group (chi 2 = 25.61, P < 0.005, RR = 4.07). The antigen HLA-Dw7 was present in 56.71% of patients compared to 15.8% in the healthy population (chi 2 = 26.55, P < 0.0001, RR = 6.98). No other significant deviations in the frequencies of investigated antigens were observed.
Assuntos
Antígeno HLA-A2/análise , Antígenos HLA-D/análise , Vitiligo/imunologia , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , EslováquiaRESUMO
24 psoriasis vulgaris patients were investigated for the expression of class II HLA antigens on the surface of PWM-stimulated T lymphocytes. The percentage of the expression of HLA-DP antigens ranged from 50.1% to 82.6% compared to a 100% level in healthy controls (p less than 0.005). No significant differences in the expression of HLA-DR antigens were observed. A higher frequency of some HLA antigens was found in the group of patients studied: B13 - 23.1%/6.2%, B 17 - 15.4%/7.1%, and Dw7 - 59.4%/15.8%.
Assuntos
Antígenos HLA-DP/biossíntese , Psoríase/imunologia , Linfócitos T/imunologia , Concanavalina A/farmacologia , Expressão Gênica , Antígenos HLA-DR/biossíntese , Humanos , Ativação Linfocitária/efeitos dos fármacos , Fito-Hemaglutininas/farmacologia , Mitógenos de Phytolacca americana/farmacologia , RadioimunoensaioRESUMO
The mitogenic response of human T lymphocytes to graded doses of concanavalin A (ConA) has been measured by means of an MTT tetrazolium dye metabolic assay. Three groups of healthy subjects, representing children, younger adults and elderly persons, were investigated. It was shown that a typical bell-shaped course of the ConA dose-response curve is the result of a proliferative response to suboptimal concentrations of ConA and a toxic action of ConA at supraoptimal concentrations. The ascending part of the response curve reflects in its shape the regulatory interaction of responding cells. A decrease in suppressive functions is accompanied by a shift of this part of the curve to lower concentrations of ConA. By means of a mathematical model derived from enzyme kinetics, an attempt was made to quantify the suppressive functions from the course of the individual dose-response curve. It was found that after suitable data processing, suppression-related shape changes can be assigned to a single parameter. The value of this parameter as a diagnostic tool was tested in a study of the age dependence of human T lymphocyte responses to ConA. While the proliferative response decreased with age, the suppressive functions exhibited their maximum effect in the group of adults. Thus it could be demonstrated that ConA induced proliferative and suppressive responses are due to two different pathways which can be independently extracted from the dose-response curve.
Assuntos
Concanavalina A/farmacologia , Ativação Linfocitária/imunologia , Linfócitos T Reguladores/imunologia , Linfócitos T/imunologia , Envelhecimento/imunologia , Anticorpos Monoclonais , Antígenos de Diferenciação de Linfócitos T/imunologia , Antígenos CD8 , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Colorimetria/métodos , Feminino , Humanos , Cinética , Ativação Linfocitária/efeitos dos fármacos , Masculino , Modelos Biológicos , Linfócitos T/efeitos dos fármacos , Linfócitos T Reguladores/efeitos dos fármacos , Sais de Tetrazólio , TiazóisRESUMO
HLA antigens and MLC reactivity were ascertained in 69 families, having altogether 198 children, with the aim to find genotypically identical donors for patients suffering from some type of leukemia or aplastic anemia. HLA identical, MLC negative sibling donors were found for 29 patients, i.e. 42.03%. In 55 leukemic patients the frequency of HLA antigens and haplotypes was calculated. No significant differences were found as compared to the healthy population. One recombination between HLA-A and HLA-B and one between HLA-B and HLA-D/DR loci were observed.
Assuntos
Medula Óssea/imunologia , Frequência do Gene , Antígenos HLA/genética , Transplante de Medula Óssea , Tchecoslováquia , Feminino , Haplótipos , Humanos , Teste de Cultura Mista de Linfócitos , Masculino , Transplante Isogênico , População Branca/genéticaAssuntos
Doadores de Sangue , Carboxihemoglobina/análise , Hemoglobinas/análise , Poluição do Ar , Tchecoslováquia , Humanos , FumarRESUMO
Investigation of a group of 23 patients suffering from subacute (de Quervain's) thyroiditis for the frequency of the HLA-Dw1 determinant showed this determinatant to be more frequent--30.4%--compared to its frequency in a healthy population--19.1%. The finding is not statistically significant.
