RESUMO
Twenty-four bacteriophages of Pseudomonas similar in terms of their morphology and structure were studied. The genomes of 23 bacteriophages from a population widespread in Belorussia and of one bacteriophage from the Krasnodar Region were shown to be double-helical DNAs with the content of GC base pairs from 46.8 to 66.7%. Four endonucleases (Eco R1, Hind III, Bam H1, and Eco RV) were used in the restriction analysis. Phage 8 which differed from the other phages in the area of its distribution was found to represent an individual group. The other phages were subdivided into eight related groups and had an identical restriction map within each group. The mean molecular masses of phage DNAs calculated by the method of summing the molecular masses of restricts ranged from 27.8 to 31.0 MDa.
Assuntos
Bacteriófagos/genética , DNA Viral/genética , Pseudomonas , DNA Viral/química , DNA Viral/metabolismo , Eletroforese em Gel de Ágar , Peso Molecular , Mapeamento por RestriçãoRESUMO
None of the 24 Pseudomonas syringae bacteriophages were found to be identical in the spectrum of lytic action. The phages were subdivided into five groups according to the number of sensitive bacterial strains and their qualitative composition.
Assuntos
Bacteriófagos/fisiologia , Lisogenia , Pseudomonas/fisiologia , Receptores Virais/fisiologiaRESUMO
Twenty-five isolates of virulent bacteriophages for Pseudomonas belonging to the morphological group C (Bradley, 1967) were obtained from different natural habitats. The phages of each isolate were found to differ from one another in at least one of the following characteristics: the sensitivity to an osmotic shock, to heating at 60 degrees C and to UV; the ability to cause lysis of 86 Pseudomonas strains; the reaction of neutralisation with antisera. At the same time, the phages were related by the existence of common antigens. These properties are responsible for the genetic stability of the bacteriophages in their complicated relationship with their host, Pseudomonas bacteria.
