Prevalence and risk factors for falls among the community dwelling older adults of Thrissur: A pilot study.

Background: According to World Health Organization (WHO) statistics, every year 28-35% of people over 65 years and 32-42% of people over 70 years experience falls. Given that many falls are preventable, can occur in any population, and can result in significant morbidity and mortality, falls are receiving more attention as a major global issue. Objective: The objectives of this study were as follows: 1. To measure the prevalence of falls among the elderly living in the Thrissur Taluk Health Centre. 2. To identify the risk factors associated with falls in the elderly using the Centre for Disease Control and Prevention, Stopping Elderly Accidents, Deaths and Injuries (CDCs STEADI) 2019 scale and the Timed Up and Go (TUG) scale. 3. To find the association between the risk factors and the prevalence of fall among older people. Materials and Methods: A cross-sectional study was conducted to find the prevalence of fall among the elderly in Thrissur Taluk Health Centers. CDCs STEADI 2019 fall risk assessment tool was used to assess the risk factors associated with the elderly. TUG test was used to determine gait, balance, strength, and posture. Results: In our study, we discovered a prevalence of 41 percent of falls among the community-dwelling older adults of Thrissur. This study has shown that the risk of fall was higher among the female elderly population. 88% are found to be at high risk of fall, and 65% of the population were worried about fall. Conclusion: This study found out a high prevalence of falls among community dwelling older adults.A future study with a larger sample size would be more helpful to confirm the impact of different variables in relation to the risk of fall among the elderly.

Androgenesis in indica rice: A comparative competency in development of doubled haploids.

Rice is critical to global food security which demands immediate attention to meet the ever-growing population. Development of improved variety is the major focus area of research, in which doubled haploid (DH) technology plays a vital role. Since, androgenesis shows its potential in DH production, this method was not capitalized specially in indica rice due to due to its recalcitrant nature to tissue culture. Success of androgenesis is governed by many important factors such as stage of anther, pre-treatment conditions, accurate concentrations of media, and plant growth regulators. Though reports of androgenesis are abundant in rice, most of them either used japonica or a specific cultivar of indica rice ecotypes. In this study, a media combination was established which is successful in producing doubled haploids from F1s of Savitri x Pokkali, IR20 x Mahulata along with the popular indica hybrids of Arize 8433DT, Arize 6453, Arize Bold, and Swift Gold. Out of 12 different media combinations tested, and 5 different durations of cold-treatments studied, N6 media with 2,4-D (2.0 mg/l) and BAP (0.5 mg/l) with 7th day cold pre-treatment was found to be most effective in all of the F1s for callus induction. Among all the F1s, rice hybrid, Arize 8433DT showed highest of 52% callus induction. In case of green shoot regeneration, MS media with NAA (0.5 mg/l), BAP (2.0 mg/l) and Kn (1.0 mg/l) (MS+C4) was found to be the most efficient of six treatments studied with highest of 58.25% regeneration in Arize 8433DT. Further, MS+C4 in combination with proline (5.0 mg/l) increased the regeneration rate to 85.99%. Besides, MS media with NAA (1.0 mg/l), Kn (0.1 mg/l) and 50 g/l sucrose was found to be most efficient for supporting root induction in all F1s. This study claims the establishment of genotype independent androgenic protocol for indica rice which could be capitalized in indica rice improvement.

First report of Fusarium proliferatum causing Sheath Rot Disease of Rice in Eastern India.

Sheath rot is one of the most devastating diseases of rice because of its ability to reduce the yield significantly in all rice cultivating areas of the world (Bigirimana et al., 2015). Sheath rot disease is associated with various pathogens such as Sarocladium oryza, Fusarium fujikuroi complex and Pseudomonas fuscovaginae (Bigirimana et al., 2015). Hence, this disease has become more complex in nature and added more seriousness. From September to December 2018, plants were observed with typical sheath rot symptoms in research farm of ICAR-National Rice Research Institute and ten farmer's fields of Cuttack district, Odisha, Eastern India. About 25 to 37% of sheath rot disease severity was recorded in the infected field. Diseased plants were observed with symptoms such as brownish or reddish brown irregular lesions, which were later, got enlarged with grayish centers. Further, rotting of the topmost leaf sheaths that surround the young panicle was observed. At the severe stages, the young panicle was partially emerged from sheath or completely rotted within the sheath. The white to pinkish powdery growth observed inside the infected sheath leading to chaffy and discolored grains. The sheath rot symptomatic plants were collected from the infected fields. To isolate the causal pathogen, infected sheath tissues were surface sterilized in 1% sodium hypochlorite for 2 min, rinsed three times in sterile distilled water, and placed on potato dextrose agar medium (PDA) (HiMedia). Plates were incubated at 27 ± 1° C for 3 d. Further, fungal pathogen colonies were sub-cultured and purified to perform the pathogenicity test. On PDA, the colonies produced abundant white aerial mycelium with violet to pink pigmentation and hyphae were hyaline with septation. Abundant single celled, oval shaped microcondia (5.5-9 × 1.5-2 µm) were produced, whereas macrocondia were not produced and the fungal pathogen was tentatively identified as Fusarium sp. In order to characterize the pathogen at molecular level, ITS, alpha elongation factor gene (EF1-α), RNA polymerase II largest-subunit gene (RPB2), calmodulin gene (cld) were amplified using the primer pair of ITS1/ITS4, EF1/EF2, 5F/7CR and CLPRO1/CLPRO2 respectively and PCR amplicons were subjected to sequencing (White et al. 1990; O'Donnell et al. 1998; Chang et al. 2015). Furthermore, a species-specific primer Fp3-F/Fp4-R was used to identify the pathogen (Jurado et al., 2006). The resulting sequences were confirmed by BLAST analysis and the FUSARIUM-ID database (http://isolate.fusariumdb.org). BLASTn search showed 100% similarity between the query sequence and ITS, EF1-α, RPB2, Calmodulin gene sequences of F. proliferatum available in the Genbank. The following GenBank accession numbers were obtained; MT394055 for ITS; MT439867 for EF1-α; MT790774 for calmodulin; MT940224 for RPB2 and MT801050 for species-specific to F. proliferatum. To confirm the pathogenicity under glass house conditions, fungus grown on sterilized chaffy grains were placed in between boot leaf sheath and panicle and covered with moist cotton (Saravanakumar et al., 2009). After 15 days post inoculation (dpi), rotting symptoms were observed and these were similar to that of field symptoms. Pathogen was constantly re-isolated from symptomatic tissue, satisfying Koch's postulates. Disease symptoms were not observed on un-inoculated plants. Morphological characters, pathogenicity test and molecular characterization have identified the pathogen as F. proliferatum. To the best of our knowledge, this is the first confirmed report of F. proliferatum causing sheath rot disease on rice from Eastern India.

Differential Expression of Genes at Panicle Initiation and Grain Filling Stages Implied in Heterosis of Rice Hybrids.

RNA-Seq technology was used to analyze the transcriptome of two rice hybrids, Ajay (based on wild-abortive (WA)-cytoplasm) and Rajalaxmi (based on Kalinga-cytoplasm), and their respective parents at the panicle initiation (PI) and grain filling (GF) stages. Around 293 and 302 million high quality paired-end reads of Ajay and Rajalaxmi, respectively, were generated and aligned against the Nipponbare reference genome. Transcriptome profiling of Ajay revealed 2814 and 4819 differentially expressed genes (DEGs) at the PI and GF stages, respectively, as compared to its parents. In the case of Rajalaxmi, 660 and 5264 DEGs were identified at PI and GF stages, respectively. Functionally relevant DEGs were selected for validation through qRT-PCR, which were found to be co-related with the expression patterns to RNA-seq. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated significant DEGs enriched for energy metabolism pathways, such as photosynthesis, oxidative phosphorylation, and carbon fixation, at the PI stage, while carbohydrate metabolism-related pathways, such as glycolysis and starch and sucrose metabolism, were significantly involved at the GF stage. Many genes involved in energy metabolism exhibited upregulation at the PI stage, whereas the genes involved in carbohydrate biosynthesis had higher expression at the GF stage. The majority of the DEGs were successfully mapped to know yield related rice quantitative trait loci (QTLs). A set of important transcription factors (TFs) was found to be encoded by the identified DEGs. Our results indicated that a complex interplay of several genes in different pathways contributes to higher yield and vigor in rice hybrids.

Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean.

Phytic acid (PA), the major phosphorus reserve in soybean seeds (60-80%), is a potent ion chelator, causing deficiencies that leads to malnutrition. Several forward and reverse genetics approaches have ever since been explored to reduce its phytate levels to improve the micronutrient and phosphorous availability. Transgenic technology has met with success by suppressing the expression of the PA biosynthesis-related genes in several crops for manipulating their phytate content. In our study, we targeted the disruption of the expression of myo-inositol-3-phosphate synthase (MIPS1), the first and the rate limiting enzyme in PA biosynthesis in soybean seeds, by both antisense (AS) and RNAi approaches, using a seed specific promoter, vicilin. PCR and Southern analysis revealed stable integration of transgene in the advanced progenies. The transgenic seeds (T4) of AS (MS14-28-12-29-3-5) and RNAi (MI51-32-22-1-13-6) soybean lines showed 38.75% and 41.34% reduction in phytate levels respectively, compared to non-transgenic (NT) controls without compromised growth and seed development. The electron microscopic examination also revealed reduced globoid crystals in the Protein storage vacoules (PSVs) of mature T4 seeds compared to NT seed controls. A significant increase in the contents of Fe2+ (15.4%, 21.7%), Zn2+ (7.45%, 11.15%) and Ca2+ (10.4%, 15.35%) were observed in MS14-28-12-29-3-5 and MI51-32-22-1-13-6 transgenic lines, respectively, compared to NT implicating improved mineral bioavailability. This study signifies proof-of-concept demonstration of seed-specific PA reduction and paves the path towards low phytate soybean through pathway engineering using the new and precise editing tools.