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Int J Mol Sci ; 12(9): 5522-37, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22016606

RESUMO

Stenocarpella maydis and Stenocarpella macrospora are the causal agents of ear rot in corn, which is one of the most destructive diseases in this crop worldwide. These fungi are important mycotoxin producers that cause different pathologies in farmed animals and represent an important risk for humans. In this work, 160 strains were isolated from soil of corn crops of which 10 showed antifungal activity against these phytopathogens, which, were identified as: Bacillus subtilis, Pseudomonas spp., Pseudomonas fluorescens, and Pantoea agglomerans by sequencing of 16S rRNA gene and the phylogenetic analysis. From cultures of each strain, extracellular filtrates were obtained and assayed to determine antifungal activity. The best filtrates were obtained in the stationary phase of B. subtilis cultures that were stable to the temperature and extreme pH values; in addition they did not show a cytotoxicity effect against brine shrimp and inhibited germination of conidia. The bacteria described in this work have the potential to be used in the control of white ear rot disease.


Assuntos
Antifúngicos/farmacologia , Ascomicetos/efeitos dos fármacos , Bactérias/metabolismo , Meios de Cultivo Condicionados/farmacologia , Antifúngicos/metabolismo , Ascomicetos/classificação , Ascomicetos/crescimento & desenvolvimento , Bacillus subtilis/genética , Bacillus subtilis/isolamento & purificação , Bacillus subtilis/metabolismo , Bactérias/classificação , Bactérias/genética , Meios de Cultivo Condicionados/metabolismo , Estabilidade de Medicamentos , Concentração de Íons de Hidrogênio , Pantoea/genética , Pantoea/isolamento & purificação , Pantoea/metabolismo , Filogenia , Pseudomonas/genética , Pseudomonas/isolamento & purificação , Pseudomonas/metabolismo , RNA Ribossômico 16S/genética , Especificidade da Espécie , Temperatura
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