Sex determination from the ribs of contemporary Turks.

There have been several in-depth studies showing that the sternal extremity of the fourth rib can be used in estimating age as well as in determining sex, as illustrated in North American whites and blacks. Yet biological differences between populations may preclude the cross-application of standards. To test this hypothesis and develop a sex determination standard for the Turkish population, a sample of the sternal ends of the fourth rib was collected from 294 individuals autopsied in Istanbul, Turkey. Dimensions from the superior edge to the inferior one (SI) and the anterior edge to its posterior edge (AP) were measured with a caliper. The sample was divided into three age groups, "young," "old," and "total." Using discriminant function analysis, three formulae were developed, one for each group. The results indicated that SI height is the most dimorphic dimension and that both dimensions together can given an accuracy of 86% to 90%. To reach such an accuracy, the age of the unknown should be estimated, at least whether it is phase 4 or above or below, using the age standards for ribs developed by Iscan and associates. When cross-validation tests are carried out, incorrect formulae reduce accuracy by as much as 20%. Furthermore, it was observed that North American based white sex determination formulae assign many Turkish males into a female category. In conclusion, it is important to note that population and age specificity are essential in determining sex from the rib. Yet its accuracy is as good as, if not better than, many bones of the postcranial skeleton.

Age assessment by rib phase analysis in Turks.

The Iscan's phase method for the estimation of adult age at death from the sternal extremity of the fourth rib was introduced in 1983. Over the years, numerous tests have confirmed the reliability of this technique on varied samples. However, no large scale study has been conducted to test the application of this method on a modern white sample geographically, genetically, and culturally diverse from the American white database. Therefore, the purpose of this paper is to apply rib phase standards to a Turkish sample to test if the progression of morphological changes follow the same age sequence. Using a sample of 150 males and 144 females of known age at death, each rib was phased using the standards developed by Iscan and associates in 1984 and 1985. The phase estimations were then subject to an analysis of variance. The results of the study indicated that Turkish ribs show the same morphological characteristics that define the phases at nearly identical ages. Variation as measured by the standard deviation increased from phase 5 on in both sexes. In conclusion, this study demonstrated that the rib phase standards can be accurately applied to Turks. Investigations of this nature are vital because one cannot assume that a method developed from one group is applicable to a distant population, especially in medicolegal proceedings.

Postgraduate forensic science education in Turkey.

Legal medicine in Turkey, has an educational background that goes back to 1839 and the first autopsy in modern terms was performed in 1841. In the early days, it was common practice for those involved in this work to extend their investigative knowledge into areas not directly concerned with medical matters. However forensic medical investigations cannot be entrusted in the hands of single investigators, but should rather be dealt with by cooperative groups of experts nowadays. This need was the major force for the establishment of the Institute of Legal Medicine and Forensic Sciences by a special article of the law (section 2547) as a training and research center in 1982. The Institute being the first and only institution giving master's and doctorate degrees in Forensic Sciences, has 3 major departments: 1) Medical Sciences Department, 2) Basic Sciences Department and 3) Social Sciences Department. Graduates of various fields ranging from medical doctors specialized in any field, biologists, chemists to lawyers, district attorneys, psychologists and other related fields are composing the multidisciplinary structure of the institute. The main research fields of the Institute are: population genetics, paternity investigation, child abuse, and identification of human remains.

Process of disintegration and degradation of M. leprae: study of tissue imprints and tissues.

The existence, distribution and behaviour of degradation products of M. leprae in leprosy lesions were investigated in tissue specimens fixed in neutral formalin and embedded in paraffin. Cytopathologic findings using tissue imprints were unsatisfactory. Sections were stained with hematoxylin-eosin, acid-fast stains, silver methenamine and by an immunochemical (PAP) technique using serial paraffin sections. A comparison in respect of the distribution of the bacilli within the macrophages showed considerable differences between the superficial and deep granulomas. This corresponds roughly with the central, intermediary and peripheral locations. In a small granuloma seen in BL lesions, there were two zones: central and peripheral. In a large LL granuloma, three zones were seen, central, intermediary and peripheral zones. It is suggested that the degradation of disintegrated particles of bacilli might be due to the lysosymal activity of macrophages. The phagocytized bacilli are slowly degraded with long incubation periods, but the undigested debris remains inside the phagosomes. The chemical complexity of cytoplasm, cell wall and lipid fractions of M. leprae, and it is such that the lipid fractions of M. leprae mask some other antigenic components, which may be responsible for the cellular response and lysosymal production. According to our findings we believe that chemotherapy kills M. leprae but degraded products are not removed. These components are chemically complex and digested with difficulty. Lysosymal enzymes could be inhibited from productions by the bacterial debris or the lipid fractions could serve as a mask to delay lysosymal production in the cell. These aspects need further study.

Lepromatous leprosy in man; depth of the cellular infiltrate and bacillary mass in relation to the possibility of transmission of leprosy by biting arthropods.

In order to further define the possible role of arthropods in the transmission of leprosy, the depth of the cellular infiltrate and bacillary mass in the skin of patients with untreated lepromatous leprosy was measured, and this was related to the depth of penetration of the mouthparts of some species of arthropods of medical importance. The results confirmed that large numbers of bacilli are readily available to the biting apparatus of several species of arthropods, but not to that of the scabies mite, which has only a superficial attachment and depth of penetration. The data do not indicate that leprosy transmission in man (or animals) occurs through biting arthropods, but they do lend support to this possibility, if only in a mechanical sense.