RESUMO
The nucleic acid-based technique has been widely utilized in many fields including for on-site detection. However, traditional molecular detection techniques encounter limitations like relying on instruments, time consuming or complex operation, and cannot meet the demands of on-site testing. In this study, a rapid DNA extraction method (RDEM), recombinase aided amplification (RAA), and chemical heating packet (CHP) are integrated and termed as RRC platform for on-site detection of nucleic acid. For demonstration purposes, SHZD32-1 (a new transgenic soybean line from China) was detected using the novel platform to demonstrate its feasibility and capability for on-site detection. Using the RDEM, high-quality DNA appropriate for molecular detection was quickly extracted in 3-5 min. The heat energy generated by CHP was met the temperature requirements of RAA. Using the RRC platform, the whole detection process can be accomplished within only 30 min, and the results can be visually detected with glasses under blue light. No special or expensive instrument was needed for the detection process. This study provides a novel approach for on-site detection of nucleic acids besides providing valuable insight on related future research.
RESUMO
OBJECTIVE: To observe the short-term therapeutic effects of Herbert screw fixation on type Mason II, Ill radial head fractures. METHODS: From March 2008 to July 2010,15 patients with Mason II, III radial head fractures were treated by open reduction and Herbert screw fixation including 6 males and 9 females with the mean age of 32 years (from 18 to 55). Seven cases were left and 8 cases were right. The interval from injury to hospitalization ranged from 3 to 10 hours. The clinical symptoms were swelling, pain, restricted movement in elbow. Bonycrepitus were heard on the elbow joint and X-ray film showed radial head fractures. Mayo elbow scoring system was used to evaluate recovery of elbow function. RESULTS: All the incisions primarily healed without myositis ossificans. All the patients were followed up for 6 to 15 months. According to Mayo elbow score, the result was excellent in 8 cases, good in 5 cases and fair in 2 cases. Mayo score was (86.67+/-1.26) points, which including pain (53.33+/-9.76), joint function(27.33+/-4.58), joint stability(6.00+/-2.07) elbow joint mobility was 70 degrees-130 degrees with the average of (105+/-10) degrees, forearm rotation was 1000-1300 with the average of (120+/-16) degrees. CONCLUSION: Treatment of Mason II, II radial head fractures with Herbert screw fixation has advantages of satisfactory reduction, reliable fixation,easy operating and early movement of joint.
