Inter-regional variation in feeding patterns of skipjack tuna (Katsuwonus pelamis) inferred from stomach content, stable isotope and fatty acid analyses.

Foraging habits of skipjack tuna, SKJ (Katsuwonus pelamis), were investigated in three Spanish marine regions (Balearic Sea, Alboran Sea and Gulf of Cadiz) using stomach content (SCA), stable isotope (SIA), and fatty acid (FA) analyses. The three methodological approaches yielded significant differences among locations. All the studied areas appear to serve as important foraging grounds for SKJ, but the diet composition significantly varied among them. The predominant prey species in the respective locations were the North Atlantic krill, Meganyctiphanes norvegica, in the Balearic Sea, the flyingfish, Exocœtus volitans, in the Alboran Sea, and the anchovy, Engraulis encrasicolus, in the Gulf of Cadiz. Regional differences were also found in δ13C and δ15N values; furthermore, the analysis of standard ellipse areas (SEAc) based on isotopic data showed that the broadest niche corresponded to SKJ from the Gulf of Cadiz, with no significant isotopic niche overlap between areas. Consistent with the SCA and stable isotope results, the FA composition varied among areas. Docosahexaenoic acid (DHA, 22:6n-3) was the most abundant FA in the SKJ muscle, showing its highest values in samples from the Gulf of Cadiz; this FA was the most effective in segregating SKJ geographical groups. In conclusion, this study provides evidence that SKJ in eastern Atlantic and western Mediterranean waters can easily adapt their diet to local prey availability. Furthermore, stable isotope signatures and FA profiles of muscle tissue prove to be reliable trophic markers that allow separation of populations occurring in different marine areas.

Molecular characterization and gene expression of thyrotropin-releasing hormone in Senegalese sole (Solea senegalensis).

We describe the cloning of a cDNA encoding TRH in Solea senegalensis. Phylogenetic analysis clustered it with other fish counterparts. Eight conserved TRH progenitors and highly divergent spacers were identified. Expression profiles of TRH were analyzed in juvenile tissues, during larval development, and in post-larvae grew under four photoperiods (12L:12D, 12D:12L, 24L:0D and 24D:0L) during four weeks using a real-time PCR approach. In juvenile fish, TRH mRNA was expressed ubiquitously although with the highest transcript levels in brain. During larval development, TRH mRNA levels were higher at 2-3days after hatching decreasing progressively until metamorphosis. Hormonal treatments using thiourea and T4 showed no regulation at transcriptional level by thyroid hormones. Moreover, no significant changes in mRNA levels between photoperiods at any time were detected. Overall, these results indicate that TRH do not participate in the regulation of HPT nor adaptation to photoperiod in sole.