RESUMO
Acca sellowiana, known as feijoa or pineapple guava, is a diploid, (2n = 2x = 22) outcrossing fruit tree species native to Uruguay and Brazil. The species stands out for its highly aromatic fruits, with nutraceutical and therapeutic value. Despite its promising agronomical value, genetic studies on this species are limited. Linkage genetic maps are valuable tools for genetic and genomic studies, and constitute essential tools in breeding programs to support the development of molecular breeding strategies. A high-density composite genetic linkage map of A. sellowiana was constructed using two genetically connected populations: H5 (TCO × BR, N = 160) and H6 (TCO × DP, N = 184). Genotyping by sequencing (GBS) approach was successfully applied for developing single nucleotide polymorphism (SNP) markers. A total of 4,921 SNP markers were identified using the reference genome of the closely related species Eucalyptus grandis, whereas other 4,656 SNPs were discovered using a de novo pipeline. The individual H5 and H6 maps comprised 1,236 and 1,302 markers distributed over the expected 11 linkage groups, respectively. These two maps spanned a map length of 1,593 and 1,572 cM, with an average inter-marker distance of 1.29 and 1.21 cM, respectively. A large proportion of markers were common to both maps and showed a high degree of collinearity. The composite map consisted of 1,897 SNPs markers with a total map length of 1,314 cM and an average inter-marker distance of 0.69. A novel approach for the construction of composite maps where the meiosis information of individuals of two connected populations is captured in a single estimator is described. A high-density, accurate composite map based on a consensus ordering of markers provides a valuable contribution for future genetic research and breeding efforts in A. sellowiana. A novel mapping approach based on an estimation of multipopulation recombination fraction described here may be applied in the construction of dense composite genetic maps for any other outcrossing diploid species.
RESUMO
Dentro del Síndrome Coronario Agudo, el Infarto Agudo de Miocardio sin onda Q, es la entidad menos estudiada en Cuba y la opción terapéutica más apropiada, es aún objeto de controversia en el mundo, de ahí la importancia de estratificar estos pacientes. Por tal motivo decidimos realizar una caracterización de los pacientes con IMA sin onda Q, según los cambios eléctricos en la fase aguda. Se estudiaron 99 pacientes, ingresados en el Hospital Militar Luis Díaz Soto, durante tres años (2001- 2003), se dividieron en 4 subgrupos, según tipos de cambios eléctricos: con supradesnivel del ST, con infradesnivel del ST, con cambios de la onda T, y sin cambios isquémicos agudos. Se describieron características epidemiológicas, topográficas y pronósticas en cada subgrupo, demostrando que no existe relación en cuanto a edad, sexo y el tipo de cambio electrocardiográfico. La Hipertensión Arterial, constituyó el factor de riesgo coronario más frecuente en los pacientes con cambios del segmento ST o EKG sin cambios isquémicos agudos. La mayor severidad y extensión del IMA, según parámetros ecocardiográficos, el grado mayor de deterioro hemodinámico, la angina recurrente, el reinfarto y la muerte, predominaron en los pacientes con infradesnivel del segmento ST constituyendo el subgrupo de peor pronóstico(AU)
Inside the Sharp Coronary Syndrome, the Sharp heart Attack of Myocardium without wave Q, is the company less studied in Cuba and the most appropriate therapeutic option, is still object of controversy in the world, from there the importance to stratify these patients. For such motive we decide to carry out a characterization of the patients with IMA without wave Q, according to the electric changes in the sharp phase. 99 patients were studied, entered in the Military Hospital Luis Díaz Thicket, for three years (2001- 2003), they were divided into 4 subgroups, according to electric exchange rates: with supradesnivel of the ST, with infradesnivel of the ST, with changes of the wave T, and without sharp ischemic changes. Topographical, epidemiological characteristics were described and pronósticas in each subgroup, showing that not relation as for age exists, sex and the exchange rate electrocardiográfico. The Arterial Hypertension, constituted the factor of most frequent coronary risk in the patients with changes of the segment ST or EKG without sharp ischemic changes. The greater severity and extension of the IMA, according to parameters ecocardiográficos, the greater degree of haemodynamic deterioration, the recurrent angina, the reinfarto and the death, they dominated in the patients with infradesnivel of the segment ST constituting the subgroup of worse forecast(AU)
