RESUMO
An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by 15N-CEST, carbonyl-13CO-CEST, aromatic-13Car-CEST, 13Cα-CEST, and methyl-13Cmet-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample.
Assuntos
Imageamento por Ressonância Magnética , Proteínas , Ressonância Magnética Nuclear Biomolecular , Conformação Proteica , Proteínas/química , Espectroscopia de Ressonância MagnéticaRESUMO
An NMR supersequence is introduced for the rapid acquisition of 15N-CEST and methyl-13C-CEST experiments in the same pulse sequence for applications to proteins. The high sensitivity and accuracy allows the simultaneous quantitative characterization of backbone and side-chain dynamics on the millisecond timescale ideal for routine screening for alternative protein states.
