RESUMO
This study was designed to determine whether the diabetic BioBreeding rat develops significant renal injury following long-term moderate to severe hyperglycaemia. Diabetic and control rats were followed from the onset of diabetes (2-4 months) to 18 months of age. Frank proteinuria and/or albuminuria were always absent. Glomerular filtration rate, measured by inulin clearance (ml min-1 (100 g body weight)-1), was significantly higher in diabetic rats than in controls at 10, 12 and 18 months of age. Advanced glycosylation end-product cross-links assessed by percentage solubility of tail tendon collagen were moderately increased in diabetic compared with control animals. Urinary excretion of advanced glycosylation end-products in unfractionated urine and in urine fractionated for low molecular mass peptides (< 10 kDa) was 11-fold greater in the diabetic rats than in the control group. Urinary excretion of nitric oxide metabolites (nmol NO2- and NO3- (24 h)-1) were significantly (P < 0.05) greater in diabetic rats than in controls after 8 months of age. Mild histopathology resembling human diabetic nephropathy, including increased mesangial volume and glomerular basement membrane thickness, was detected at 18 months of age. The findings of hyperfiltration and mild glomerular morphological changes in diabetic BioBreeding rats are similar to the abnormalities seen in stage 2 human diabetic nephropathy. We hypothesize that two factors which may contribute to the resistance or tolerance to renal injury in the BioBreeding diabetic rat are increased nitric oxide production and the decreased accumulation of advanced glycosylation end-products.
Assuntos
Diabetes Mellitus/patologia , Diabetes Mellitus/fisiopatologia , Glomérulos Renais/patologia , Ratos Endogâmicos BB/fisiologia , Animais , Diabetes Mellitus/urina , Produtos Finais de Glicação Avançada/metabolismo , Rim/fisiopatologia , Masculino , Ratos , Fatores de TempoAssuntos
Coreia/etiologia , Glomerulonefrite/complicações , Faringite/complicações , Febre Reumática/complicações , Infecções Estreptocócicas/complicações , Streptococcus pyogenes , Pré-Escolar , Coreia/tratamento farmacológico , Feminino , Humanos , Penicilinas/uso terapêutico , Faringite/diagnóstico , Faringite/tratamento farmacológico , Fenobarbital/uso terapêutico , Infecções Estreptocócicas/diagnóstico , Infecções Estreptocócicas/tratamento farmacológico , Fatores de TempoRESUMO
The renal handling of sodium and calcium in spontaneously hypertensive rats (SHR) was investigated over an extended period (10-75 weeks of age) and compared with age-matched normotensive Wistar-Kyoto controls. The animals were fed a standard rat chow except during screening periods when liquid diet that matched the pellet chow was substituted. Sodium balance, urinary excretion of sodium and calcium, fractional excretion of sodium (FENa), and renal cortical dopamine receptors (DA1 and DA2) were measured at 10, 30, 60 and 75 weeks of age. The results showed no difference between the two strains except for FENa, which was significantly higher in the SHR at 75 weeks coincident with decreased glomerular filtration rate. We conclude that a defect in renal handling of sodium and/or calcium is not a major factor in the maintenance of hypertension in the SHR.
Assuntos
Cálcio/urina , Hipertensão/fisiopatologia , Rim/fisiopatologia , Sódio/urina , Animais , Córtex Renal/química , Masculino , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Receptores Dopaminérgicos/análiseRESUMO
Rats of the spontaneously hypertensive strain develop kidney damage that resembles the nephropathy seen in some cases of human essential hypertension. Previous studies with a triple drug antihypertensive regimen indicated that proteinuria and glomerular histopathology in spontaneously hypertensive rats might develop despite long-term effective control of systemic blood pressure. To investigate further the relation between hypertension and kidney disease, a group of spontaneously hypertensive rats were treated with enalapril at 15 weeks of age. Blood pressure, protein excretion, and kidney function were measured in those rats at regular intervals during the next year and a half and were compared with untreated spontaneously hypertensive rats and the normotensive Wistar-Kyoto parent strain. Kidney tissue samples from all three groups, collected at autopsy, were stained by immunohistochemical and conventional methods to assess the relative severity and nature of kidney damage. Although enalapril therapy was completely effective in controlling the blood pressure of spontaneously hypertensive rats, it only postponed the onset of kidney disease. Enalapril-treated spontaneously hypertensive rats eventually exhibited albuminuria as severe as that found in hypertensive rats. Kidney vessel pathology was completely prevented with enalapril, but the abnormal accumulation of mononuclear cells in tubulointerstitial and periglomerular sites was the same as in untreated spontaneously hypertensive rats. We have concluded that elevated protein excretion in rats of the spontaneously hypertensive rat strain is not a secondary consequence of systemic hypertension. Structural abnormalities of renal vessels also do not appear to contribute significantly to the pathogenesis of albuminuria in spontaneously hypertensive rats. Other explanations must be sought to account for the close link between spontaneous hypertension and kidney damage in this animal model. The clear dissociation of kidney disease from systemic hypertension exhibited by spontaneously hypertensive rats may also be relevant for human disease.
Assuntos
Enalapril/uso terapêutico , Hipertensão/tratamento farmacológico , Nefropatias/prevenção & controle , Albuminúria/prevenção & controle , Animais , Proteínas Alimentares/administração & dosagem , Taxa de Filtração Glomerular , Hipertensão/complicações , Hipertensão/fisiopatologia , Rim/patologia , Rim/fisiopatologia , Masculino , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKYRESUMO
Derangements of fluid, electrolyte, and acid-base homeostasis are an inevitable part of acute renal failure. Understanding the pathophysiology of these disorders is essential to treating and preventing potentially life-threatening complications. Appropriate nutritional support is also an important part of management in childhood acute renal failure.
Assuntos
Injúria Renal Aguda/terapia , Hidratação , Desequilíbrio Ácido-Base/terapia , Injúria Renal Aguda/fisiopatologia , Líquidos Corporais/fisiologia , Criança , Transtornos da Nutrição Infantil/terapia , Humanos , Desequilíbrio Hidroeletrolítico/terapiaRESUMO
A newborn infant with abdominal masses was found to have Glomerulocystic Kidney Disease. Imaging showed markedly enlarged kidneys with multiple macroscopic cysts. Radiographic and clinical findings are discussed.
Assuntos
Doenças Renais Císticas/congênito , Doenças Renais Císticas/diagnóstico , Glomérulos Renais/patologia , Humanos , Recém-Nascido , Doenças Renais Císticas/diagnóstico por imagem , Imageamento por Ressonância Magnética , Masculino , Tomografia Computadorizada por Raios X , UltrassonografiaRESUMO
A combination of metolazone (0.2 mg/kg/day) and furosemide (4 mg/kg/day) was used in the treatment of a 2-week-old neonate who developed severe edema after cardiac surgery. The edema, which was initially responsive to furosemide, became resistant to high doses of this diuretic even with the concomitant use of ethacrynic acid. The addition of metolazone to furosemide induced prompt diuresis and natriuresis. This combination of diuretics can be helpful in the treatment of refractory edema in young infants.