Assuntos
Anomuros , Aquicultura/métodos , Penaeidae/enzimologia , Penaeidae/fisiologia , Fenômenos Fisiológicos da Nutrição Animal , Animais , Proteínas de Artrópodes/genética , Proteínas de Artrópodes/metabolismo , Trato Gastrointestinal/enzimologia , Regulação da Expressão Gênica , México , Análise de Sequência com Séries de Oligonucleotídeos/veterinária , Penaeidae/genética , Penaeidae/crescimento & desenvolvimento , Reação em Cadeia da Polimerase/veterináriaRESUMO
To assess the immunogenic and immunoprotective role of the extracellular lectin from Aeromonas veronii (MCBP), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (Paralabrax maculatofasciatus) were orally immunised either with soluble MCBP, adjuvant-conjugated MCBP or immobilised MCBP on latex microspheres. The results suggest that the MCBP is capable of eliciting protective immunity against A. veronii infections when administered orally. The highest mucosal immune response was elicited in fish immunised with MCBP covalently linked to cholera toxin B subunit (CTB) or to Escherichia coli heat-labile toxin (hLT). MCBP-CTB was found to elicit immunoprotection against a challenge with live Aeromonas cells with a relative percent survival of almost 70% and without the expression of the severe histopathological alterations induced by A. veronii.
