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Reprod Domest Anim ; 50(3): 526-8, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25704292

RESUMO

Fifty-seven genital tracts of regularly slaughtered culled Piedmontese cows, aged 7.4 ± 4.3 years (mean ± SD), range: 2.6-15.6 years, were grossly and microscopically examined. DNA extracted from oviducts was subjected to PCR to evaluate the presence of Chlamydia spp. The 15 PCR-positive oviducts were subjected to Sanger sequencing and showed the presence of Chamydia abortus, with an identity range between 99 and 100%. Nine of the PCR-positive samples belonged to the 24 animals with a normal macroscopic appearance of the whole genital tract (percentage of positive oviducts in normal genital tracts 9/24 = 37.5%), while six belonged to the 33 genital tracts with lesions in one or more organs (percentage of positive oviducts in pathological genital tracts 6/33 = 18.1%); of these, a single animal had salpingitis. The detection of C. abortus in bovine oviducts is of particular interest because it has never been previously investigated or reported.


Assuntos
Doenças dos Bovinos/microbiologia , Infecções por Chlamydia/veterinária , Chlamydia/isolamento & purificação , Oviductos/microbiologia , Animais , Bovinos , Chlamydia/classificação , Infecções por Chlamydia/diagnóstico , Feminino
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