RESUMO
Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three other isolates were strongly cytotoxic for 3T3 cells but only slightly so for BRL 3A cells. The remaining three isolates showed no cytotoxicity for either cell line. The cytotoxic products were greater than 100 kDa in mol. wt, thermolabile, and partly destroyed by trypsin treatment. The data show that some B. piliformis isolates produce cytotoxic proteins, which may contribute to the pathogenesis of Tyzzer's disease.
Assuntos
Bacillus/patogenicidade , Citotoxinas/biossíntese , Fibroblastos/microbiologia , Fígado/microbiologia , Animais , Linhagem Celular , Enterite/microbiologia , Enterite/veterinária , Infecções por Bactérias Gram-Positivas/microbiologia , Infecções por Bactérias Gram-Positivas/veterinária , Células HeLa , Hepatite Animal/microbiologia , Humanos , Fígado/citologia , Síndrome , TemperaturaRESUMO
Copper sulfate and a metastabilized chlorous acid/chlorine dioxide (MECA) compound were evaluated for efficacy in treating Trichophyton mentagrophytes infection in rabbits. Forty rabbits naturally infected with T mentagrophytes were allotted to 5 treatment groups: nontreated control, sham dip, 1% copper sulfate dip, MECA dip, and MECA spray. Results of lesion culture, regression of gross lesions, and histopathologic findings were documented. Copper sulfate and MECA were effective in treating clinical dermatophytosis and in decreasing T mentagrophytes carrier status in rabbits.
Assuntos
Cloretos/uso terapêutico , Compostos Clorados , Cloro/uso terapêutico , Cobre/uso terapêutico , Óxidos/uso terapêutico , Coelhos , Tinha/veterinária , Administração Tópica , Aerossóis , Animais , Cloretos/administração & dosagem , Cloro/administração & dosagem , Cobre/administração & dosagem , Sulfato de Cobre , Desinfetantes/administração & dosagem , Desinfetantes/uso terapêutico , Feminino , Masculino , Óxidos/administração & dosagem , Tinha/tratamento farmacológico , Trichophyton/isolamento & purificaçãoRESUMO
Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.
