RESUMO
The recently identified atypical porcine pestivirus (APPV) was demonstrated to be the causative agent of the neurological disorder "congenital tremor" in newborn piglets. Despite its relevance and wide distribution in domestic pigs, so far nothing is known about the situation in wild boar, representing an important wild animal reservoir for the related classical swine fever virus. In this study, 456 wild boar serum samples obtained from northern Germany were investigated for the presence of APPV genomes and virus-specific antibodies. Results of real-time RT-PCR analyses revealed a genome detection rate of 19%. Subsequent genetic characterization of APPV (n = 12) from different hunting areas demonstrated close genetic relationship and, with exception of APPV from one location, displayed less than 3.3% differences in the analysed partial NS3 encoding region. Furthermore, indirect Erns ELISA revealed an antibody detection rate of approx. 52%, being in line with the high number of viremic wild boar. Analysis of fifteen wild boar samples from the Republic of Serbia by Erns antibody ELISA provided evidence that APPV is also abundant in wild boar populations outside Germany. High number of genome and seropositive animals suggest that wild boar may serve as an important virus reservoir for APPV.
Assuntos
Reservatórios de Doenças/veterinária , Genoma Viral , Infecções por Pestivirus/veterinária , Pestivirus/genética , Sus scrofa/virologia , Doenças dos Suínos/virologia , Animais , Anticorpos Antivirais/sangue , Reservatórios de Doenças/virologia , Ensaio de Imunoadsorção Enzimática/veterinária , Alemanha , Pestivirus/imunologia , Pestivirus/isolamento & purificação , Infecções por Pestivirus/virologia , Filogenia , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Suínos , Viremia/virologiaRESUMO
The purpose of the study was to investigate the antihypertensive effect and the dependability of labetalol in 21 essential hypertensive patients. After a period of one week without drugs, the patients were first given placebo for one week and subsequently 200 mg or 400 mg labetalol daily for eight weeks. Lipid assays were performed and renal and hepatic functions determined before and after therapy. In conclusion, it was shown that labetalol effectively controls blood pressure and reduces heart rate without causing any changes in renal and hepatic functions and lipid metabolism.
Assuntos
Hipertensão/tratamento farmacológico , Labetalol/uso terapêutico , Adulto , Pressão Sanguínea/efeitos dos fármacos , Feminino , Frequência Cardíaca/efeitos dos fármacos , Humanos , Hipertensão/sangue , Hipertensão/fisiopatologia , Labetalol/efeitos adversos , Lipídeos/sangue , Masculino , Pessoa de Meia-IdadeRESUMO
The value of demonstrating the venous phase of angiography in patients with subclavian-steal syndrome was evaluated in 17 patients. In patients with mild symptoms, there was no difference between the left and right jugular-vein opacification, but minimal or no opacification was observed in patients with severe symptoms. The authors believe evaluating venous phases in angiography in patients with subclavian-steal syndrome is useful because the findings correlate well with the severity of symptoms.
