RESUMO
This study assess the quality of wastewater through the detection and quantification of important viruses causing gastroenteritis at different stages of the wastewater treatment process in an activated-sludge wastewater treatment plant with ultraviolet disinfection. Ten sampling events were carried out in a campaign along a period of 18 months collecting wastewater samples from the influent, after the activated-sludge treatment, and after the final disinfection with UV radiation. Samples were concentrated through ultracentrifugation and analysed using retro-transcription, PCR and real time quantitative PCR protocols, for detection and quantification of Group A Rotavirus (RVA), Human Astrovirus (HAstV), Norovirus Genogroup II (NoV GII) and Human Adenovirus (HAdV). HAdV (100%), NoV GII (90%), RVA (70%) and HAstV (60%) were detected in influent samples with concentration from 1·4 (NoV GII) to 8·0 (RVA) log10 gc l-1 . Activated-sludge treatment reached well quality effluents with low organic material concentration, although nonstatistical significant differences were registered among influent and postactivated sludge treatment samples, regarding the presence and concentration for most viruses. All post-UV samples were negative for NoV GII and HAstV, although RVA and HAdV were detected in 38% and 63% of those samples respectively, with concentration ranging from 2·2 to 5·5 and 3·1 to 3·4 log10 gc l-1 . SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates that an activated-sludge wastewater treatment plant with UV disinfection reduces to levels below the detection limit those single-stranded RNA viruses as noroviruses and astroviruses and reach significant lower levels of rotaviruses and adenoviruses after the complete treatment process.
Assuntos
Adenovírus Humanos/efeitos da radiação , Desinfecção/métodos , Enterovirus/efeitos da radiação , Mamastrovirus/efeitos da radiação , Norovirus/efeitos da radiação , Rotavirus/efeitos da radiação , Esgotos/virologia , Raios Ultravioleta , Adenovírus Humanos/genética , Adenovírus Humanos/isolamento & purificação , Surtos de Doenças/prevenção & controle , Enterovirus/genética , Enterovirus/isolamento & purificação , Gastroenterite/virologia , Humanos , Mamastrovirus/genética , Mamastrovirus/isolamento & purificação , Norovirus/genética , Norovirus/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Rotavirus/genética , Rotavirus/isolamento & purificação , Uruguai , Purificação da Água/métodosRESUMO
OBJETIVO: Estudiar la relación que existe entre administración de analgesia epidural durante el trabajo de parto y parto instrumentado (con fórceps o ventosa) para los casos estudiados. MATERIAL Y MÉTODOS: Se seleccionaron 380 casos aleatoriamente a partir de la revisión de historias clínicas, de los cuales 190 habían recibido analgesia epidural y el resto (190) no lo habían hecho. RESULTADOS: La instrumentalización del parto es mayor entre las mujeres que reciben analgesia epidural (un 46,3 frente a un 24,2 por ciento de instrumentalización sin dicha analgesia; p = 0,000). Por otra parte, se registró una frecuencia similar de casos de sufrimiento fetal intraparto para ambos grupos, (un 5,8 por ciento de los partos con analgesia epidural y un 5,3 por ciento de los casos sin analgesia epidural; p > 0,05).CONCLUSIÓN: Los resultados sugieren que el impacto de la analgesia epidural sobre el desarrollo último del parto podría verse influido por un cambio en la práctica obstétrica (AU)
Assuntos
Adolescente , Adulto , Feminino , Humanos , Analgesia Epidural/classificação , Analgesia Epidural/métodos , Parto/classificação , Parto/métodos , Analgesia Obstétrica/métodos , Cuidados Pré-Operatórios/métodos , Cuidados Pré-Operatórios , Analgesia/instrumentação , Analgesia/métodos , Estudos Retrospectivos , Paridade/fisiologia , Trabalho de Parto Induzido/métodos , Analgesia Epidural/tendênciasRESUMO
Neurotrophins, such as NGF, BDNF and NT-3 play a regulatory role on the function of microglial cells in vivo and in vitro, and the identification of new compounds with neurotrophic properties is becoming a new strategy for the prevention and/or treatment of neurodegenerative disorders. In this study we describe the use of two different models to demonstrate the ability of Cerebrolysin to reduce microglial activation. The results of these in vitro and in vivo studies indicate that Cerebrolysin might exert a neuroimmunotrophic activity reducing the extent of inflammation and accelerated neuronal death under pathological conditions such as those observed in neurodegenerative diseases.
