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1.
Eur J Neurol ; 26(7): 961-968, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30742740

RESUMO

BACKGROUND AND PURPOSE: The aim of this study was to examine the association amongst remote diffusion-weighted imaging lesions (R-DWILs), imaging markers of cerebral small vessel disease (cSVD) and total cSVD burden in patients with primary intracerebral haemorrhage (ICH). METHODS: In total, 344 consecutive primary ICH patients were enrolled prospectively. R-DWILs on magnetic resonance imaging as well as four imaging markers of cSVD, including cerebral microbleeds (CMBs), white matter hyperintensities (WMHs), lacunes and enlarged perivascular spaces, were rated with validated scales. The total cSVD score was calculated by adding up these four markers. Univariate and multivariate analyses were performed. RESULTS: Remote DWI lesions were detected in 57 (16.6%) primary ICH patients. On multivariate logistic regression analysis, the presence of CMBs [odds ratio (OR) 5.26, 95% confidence interval (CI) 1.72-16.12], of high-grade WMHs (OR 4.68, 95% CI 2.01-10.90), the presence of lacunes (OR 2.69, 95% CI 1.20-6.06), mixed CMBs (OR 2.93, 95% CI 1.35-6.36), mixed lacunes (OR 3.60, 95% CI 1.25-10.37), periventricular WMHs (OR 2.19, 95% CI 1.40-3.44), deep WMHs (OR 1.92, 95% CI 1.24-2.97) and total WMHs (OR 1.52, 95% CI 1.20-1.94) were associated with the presence of R-DWILs. A significant association was also found between high-grade total cSVD score and R-DWILs (OR 1.97, 95% CI 1.36-2.84). This association remained significant in patients stratified by an age of 60 years or more than 60 years. CONCLUSIONS: Remote DWI lesions are correlated with the severity of each imaging marker of cSVD and with the total burden of cSVD.


Assuntos
Encéfalo/diagnóstico por imagem , Hemorragia Cerebral/diagnóstico por imagem , Doenças de Pequenos Vasos Cerebrais/diagnóstico por imagem , Imagem de Difusão por Ressonância Magnética , Idoso , Idoso de 80 Anos ou mais , Hemorragia Cerebral/complicações , Doenças de Pequenos Vasos Cerebrais/complicações , Feminino , Humanos , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade
2.
J Vet Med Sci ; 61(10): 1161-5, 1999 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-10563297

RESUMO

We determined the nucleotide sequence of non-pathogenic Marek's disease virus serotype 2 (MDV2) strain HPRS24 glycoprotein B (gB) (UL27), ICP18.5 (UL28) and major DNA-binding protein (MDBP) (UL29) genes homologous to herpes simplex virus type 1 (HSV-1). The sequence data revealed that important motives in the proteins are conserved in MDV2 ICP18.5 and MDBP, however the sequence of viral DNA replication origin which exists in the regions between the UL29 and UL30 genes of other alphaherpesviruses was not found in the regions of the MDV2 genome. By northern blot analyses, we also demonstrated that 8.9, 5.0 and 2.6 kb transcripts were actually transcribed from the sequenced region in MDV2-infected cells. The MDV2 UL28 and UL29 genes have not been reported in other serotypes of MDV.


Assuntos
Herpesvirus Humano 1/genética , Herpesvirus Galináceo 2/genética , Doença de Marek/genética , Sequência de Aminoácidos , Animais , Northern Blotting , Galinhas , Sequência Consenso , Sondas de DNA/química , Eletroforese em Gel de Ágar , Biblioteca Gênica , Herpesvirus Humano 1/química , Herpesvirus Galináceo 2/química , Dados de Sequência Molecular , RNA Viral/isolamento & purificação , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
3.
J Vet Med Sci ; 61(7): 787-93, 1999 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10458102

RESUMO

We identified and determined the nucleotide sequence of Marek's disease virus serotype 2 (MDV2) UL25, UL26 and UL26.5 homologous genes of herpes simplex virus type 1 (HSV-1). The UL25, UL26 and UL26.5 genes of HSV-1 encode virion proteins (UL25 and UL26.5) and serine protease (UL26). The deduced amino acid sequences of the three proteins show a high degree of homology to counterparts of HSV-1. By northern blot analyses we found that four transcripts whose sizes are 4.9, 3.9, 2.0 and 1.3 kb are transcribed from the domains of MDV2 genome containing the three genes. This is the first report dealing with UL25, UL26 and UL26.5 homologues of HSV-1 in MDV serotypes.


Assuntos
Capsídeo/genética , Herpesvirus Galináceo 2/genética , Serina Endopeptidases/genética , Simplexvirus/genética , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA Viral/genética , Herpesvirus Galináceo 2/classificação , Dados de Sequência Molecular , Fases de Leitura Aberta , Mapeamento por Restrição , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Sorotipagem , Transcrição Gênica
4.
J Vet Med Sci ; 61(6): 587-93, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10423678

RESUMO

We determined 3,135 bp of the nucleotide sequence located in an 8.5 kb EcoRI-E fragment in the unique long (UL) genome region of Marek's disease virus serotype 2 (MDV2), and identified UL20 and UL21 homologous genes of herpes simplex virus type 1 (HSV-1). The UL20 and UL21 homologous genes of MDV2 are arranged colinearly with the prototype sequence of HSV-1. In addition, an open reading frame (MDV2 ORF 273), which has been identified within the UL21 homologous gene of MDV2, has no apparent relation to any other known herpesvirus genes. Northern blot analysis and reverse transcriptase polymerase chain reaction confirmed the existance of RNA transcripts related to the UL20 and ORF 273 genes in MDV2-infected cells, except no transcript related to the UL21 gene being detected. The putative protein product of the MDV2 UL20 gene had a relatively low homology but that of the MDV2 UL21 gene had a moderate homology among herpesviruses. Further, the possible functions and features of the predicted proteins encoded within the sequenced region are discussed.


Assuntos
Herpesvirus Galináceo 2/genética , Análise de Sequência de DNA , Proteínas Virais/genética , Sequência de Aminoácidos , Sequência de Bases , Vetores Genéticos , Genoma Viral , Herpesvirus Humano 1/genética , Herpesvirus Galináceo 2/classificação , Dados de Sequência Molecular , Sorotipagem
5.
J Vet Med Sci ; 61(6): 683-7, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10423692

RESUMO

The gene of Marek's disease virus serotype 2 (MDV2) homologous to the UL52 gene of herpes simplex virus type 1 (HSV-1) was identified and characterized. The MDV2 UL52 homologous gene encodes 1,071 amino acids with a molecular weight of 118.7 kDa, which includes putative metal-binding site and overlapping region with the UL53 homologous gene. Although a putative polyadenylation signal sequence was found in the downstream of the MDV2 UL52 gene, a MDV2 UL52 DNA probe reacted only with the polycistronic 6.3 kb transcript, representing the UL52 and the downstream genes of UL53 and UL54. Transcriptional pattern of this region of MDV2 was somewhat different from corresponding regions of HSV-1 and infectious laryngotracheitis virus.


Assuntos
DNA Helicases/genética , Herpesvirus Galináceo 2/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Primase , Genoma Viral , Biblioteca Genômica , Herpesvirus Humano 1/genética , Herpesvirus Galináceo 2/classificação , Dados de Sequência Molecular , Análise de Sequência de DNA , Sorotipagem , Proteínas Virais
6.
J Vet Med Sci ; 61(6): 701-3, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10423696

RESUMO

Recently we cloned 140 kDa form of feline CD56 cDNA. In this study, we expressed the feline CD56 molecule by the baculovirus expression system. We found that the molecule was expressed on the cell surface when examined by the indirect immunofluorescence assay using an anti-human CD56 monoclonal antibody. Immunoblotting analysis revealed that the molecular weight of the major expressed product was 140 kDa. Interestingly we found that the insect cells expressing the feline CD56 molecule aggregated, indicating that the expressed molecule mediates homophilic adhesion.


Assuntos
Baculoviridae , Antígeno CD56/genética , Animais , Anticorpos Monoclonais , Antígeno CD56/química , Antígeno CD56/metabolismo , Gatos , Linhagem Celular , Clonagem Molecular/métodos , DNA Complementar , Eletroforese em Gel de Poliacrilamida , Técnica Indireta de Fluorescência para Anticorpo , Immunoblotting , Insetos/citologia
7.
J Vet Med Sci ; 61(5): 503-11, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10379942

RESUMO

We determined the nucleotide sequence of a portion of BamHI-C fragment of Marek's disease virus serotype 2 (MDV2) strain HPRS24 which was suspected to contain the homologue of the herpes simplex virus type 1 (HSV-1) gene UL10, encoding glycoprotein M (gM). An open reading frame whose translation product exhibited significant similarities to HSV-1 gM protein and respective proteins of other herpesviruses of 37.5% and 45.5% to 31.8%, respectively, was identified. A number of distinct transcriptional consensus sequences were found upstream of the first putative start codon of MDV2 UL10 protein. In transcriptional analysis, the gene was transcribed into an 1.5 kb RNA. The primary translation product comprises 424 amino acids with a predicted molecular weight of 46.9 kDa. The predicted MDV2 UL10 protein contains eight hydrophobic domains with sufficient length and hydrophobicity to span the lipid bilayer conserved in the genomes of all herpesviruses which have been sequenced so far. In the region located between the first and second hydrophobic domains, two potential N-linked glycosylation sites were presented. Interestingly, highly charged residues were abundantly possessed in the carboxy-terminal part of the MDV2 UL10 protein. By comparison of the amino acid sequence of the MDV2 UL10 gene with the homologues from other herpesviruses, the data might contribute for further evidence of the evolution of herpesviruses from a common progenitor and an ancient example of MDV2 belonging to the Alphaherpesvirinae subfamily. In addition, the existence of corresponding genes in human, mammalian, and avian herpesvirus genomes, suggests indirectly an important role for gM in the natural life cycle of the virus.


Assuntos
Glicoproteínas/genética , Herpesvirus Humano 1/genética , Herpesvirus Galináceo 2/genética , Glicoproteínas de Membrana/genética , Proteínas de Membrana/genética , Proteínas Virais/genética , Sequência de Aminoácidos , Sequência de Bases , Sequência Consenso , Sequência Conservada , Herpesviridae/genética , Humanos , Glicoproteínas de Membrana/química , Proteínas de Membrana/química , Dados de Sequência Molecular , Fases de Leitura Aberta , Biossíntese de Proteínas , Mapeamento por Restrição , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Transcrição Gênica , Proteínas Virais/química
8.
Virus Res ; 60(1): 101-10, 1999 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10225279

RESUMO

We determined the nucleotide sequence of a 6593 bp fragment of the Marek's disease virus serotype 2 (MDV2) unique long region located in the right part of genomic BamHI-M2 and the adjacent part of BamHI-K fragments. Within this region five complete open reading frames (ORFs) were identified whose deduced amino acid sequences exhibited homology to the UL53 (glycoprotein K), UL54 (immediate early regulatory protein ICP27), and UL55 gene products of herpes simplex virus type 1 (HSV-1). Homologue to the HSV-1 UL56 was not detected. However, we identified a gene between the MDV2 UL54 and UL55 genes with homology to the first ORF (ORF-1) of equine herpesvirus type 1 and corresponding gene identified in pseudorabies virus. Two adjacent ORFs contained in the BamHI-K fragment, ORF 873s and ORF 873, were found by computer analysis to have the properties of an intron encoding a glycoprotein: ORF 873s encodes a 84 amino acid polypeptide with a stretch of a hydrophobic signal sequence in the C-terminus, and ORF 873 encodes a 873 amino acid polypeptide with a transmembrane domain and putative three N-linked glycosylation sites. All the identified genes were confirmed to be transcribed with 3'-coterminal transcripts and/or a unique transcript in the virus-infected cells. Especially, 3.5 kb mRNA of ORF 873s and ORF 873 are transcribed from a potential promoter region of ORF 873s, and splice donor and acceptor sites are used to splice the mRNA after cleavage of a 113 bp-nucleotide sequence.


Assuntos
Rearranjo Gênico , Genoma Viral , Herpesvirus Galináceo 2/genética , Transcrição Gênica , Sequência de Aminoácidos , Animais , Desoxirribonuclease BamHI , Humanos , Dados de Sequência Molecular , RNA Viral , Homologia de Sequência de Aminoácidos , Sorotipagem , Proteínas do Envelope Viral/genética
10.
Vet Immunol Immunopathol ; 65(1): 43-50, 1998 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-9802575

RESUMO

The cDNA of feline CD3epsilon, one of the T-cell receptor components, was cloned from a feline T-lymphoblastoid cell line (MYA-1 cells) and peripheral blood mononuclear cells and thymocytes of cats by polymerase chain reaction. Sequencing analysis revealed that the open reading frame of feline CD3epsilon consists of 606 base pairs encoding a predicted molecular mass of 25 kDa transmembrane protein which lacks N-glycosylation site. Comparison of the predicted amino acid sequence of feline CD3epsilon with those of other mammalians' homologues revealed that a relatively low homology was present in the extracellular domain. However, the cytoplasmic domain contained several characteristic motifs highly conserved across the species. These motifs were known to be important for signal transduction upon T-cell activation or endoplasmic reticulum retention. In addition, the feline CD3epsilon protein was expressed in an insect cell line (Sf9) by a baculovirus expression system. The expression was confirmed by indirect immunofluorescence assay and immunoblotting analysis using an anti-human CD3epsilon polyclonal antibody. These results will provide additional information for understanding the feline immune system.


Assuntos
Complexo CD3/genética , Gatos/imunologia , Regulação da Expressão Gênica , Sequência de Aminoácidos , Animais , Baculoviridae/química , Sequência de Bases , Western Blotting/veterinária , Complexo CD3/química , Clonagem Molecular , Primers do DNA/química , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Humanos , Dados de Sequência Molecular , Peso Molecular , Fases de Leitura Aberta , RNA Mensageiro/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterinária , Homologia de Sequência de Aminoácidos , Transfecção/genética
11.
Eur J Immunogenet ; 25(4): 303-5, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9777331

RESUMO

The stromal cell-derived factor-1 alpha and beta (SDF-1 alpha/beta) are the ligands of fusin/CXCR4, the co-receptors of human immunodeficiency virus type 1 and the feline immunodeficiency virus. We cloned the cDNA of feline SDF-1 alpha/beta. The open reading frames of feline SDF-1 alpha/beta were 267/279 base pairs and encoded 89/93 amino acid residues.


Assuntos
Quimiocinas CXC/genética , Citocinas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Gatos , Quimiocina CXCL12 , Clonagem Molecular , DNA Complementar , Humanos , Camundongos , Dados de Sequência Molecular , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
12.
J Gen Virol ; 79 ( Pt 8): 1997-2001, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9714249

RESUMO

Studies on Marek's disease virus serotype 2 (MDV2) are important for understanding the natural nonpathogenic phenotypes of MDV. We determined the 16770 bp nucleotide sequence of the MDV2 genome located in the right part the of unique long region. The analysis revealed 12 complete open reading frames (ORFs) with high amino acid sequence identities to the gene products of other alphaherpesviruses. The MDV2 ORFs were arranged collinearly with the prototype sequence of herpes simplex virus type 1 ranging from the UL41 to UL51 genes. Except for the MDV2 UL41 gene, all of the identified genes were confirmed to be transcribed with 3'-coterminal mRNAs and/or a unique transcript in the virus-infected cells. Transcriptional patterns for the regions of the MDV2 UL48 to UL49.5 genes were notably different from the similar area of MDV serotype 1.


Assuntos
Genoma Viral , Herpesvirus Galináceo 2/genética , Transcrição Gênica , Animais , Sequência de Bases , Mapeamento Cromossômico , DNA Viral , Genes Virais , Herpesvirus Humano 1/genética , Herpesvirus Galináceo 2/classificação , Dados de Sequência Molecular , Sorotipagem , Proteínas Virais/genética
13.
J Vet Med Sci ; 58(11): 1057-66, 1996 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8959652

RESUMO

In the Marek's disease virus (MDV) serotype 2 (MDV2) genome, a gene equivalent to the glycoprotein I (gI) of other alphaherpesviruses was identified and sequenced. The primary translation product comprises 355 amino acids with a M(r) of 38.4 kDa. The predicted amino acid sequence possesses several characteristics typical of membrane glycoproteins, including a N-terminal hydrophobic signal sequence, C-terminal transmembrane and cytoplasmic domains, and extra-cellular region containing three potential N-linked glycosylation sites. Compared to other MDV serotypes, MDV2 gI showed 49% identity with MDV1 gI, and 36% identity with HVT gI at the amino acid level. In transcriptional analyses, a 3.5 kb mRNA which starts between 56 and 147 bps upstream of the potential translational initiation codon of gI was identified as the gI-specific transcript. By a recombinant baculovirus, this potential gI encoding region was expressed as two specific products 45 and 43 kDa. Both products were susceptible to tunicamycin treatment, indicating that they were glycoprotein. Further, the expressed gI reacted with all chicken-antisera raised to each of the three serotypes of MDV (strains GA, SB-1, and FC126), suggesting that gI is expressed by all three serotypes of MDV in infected cells and conserves common antigenic epitope(s) beyond serotypes.


Assuntos
Baculoviridae/genética , Galinhas/virologia , Glicoproteínas/genética , Herpesvirus Galináceo 2/genética , Proteínas Virais/genética , Sequência de Aminoácidos , Aminoácidos/análise , Animais , Antivirais/farmacologia , Sequência de Bases , Northern Blotting/veterinária , Reações Cruzadas , DNA Viral/análise , DNA Viral/química , DNA Viral/genética , Epitopos/análise , Epitopos/imunologia , Imunofluorescência/veterinária , Amplificação de Genes , Regulação Viral da Expressão Gênica , Glicoproteínas/análise , Glicoproteínas/química , Glicosilação , Dados de Sequência Molecular , Testes de Precipitina/veterinária , RNA Mensageiro/análise , RNA Mensageiro/química , RNA Mensageiro/genética , Homologia de Sequência de Aminoácidos , Transcrição Gênica , Tunicamicina/farmacologia , Proteínas Virais/análise , Proteínas Virais/química
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