[Comparative study on HPLC fingerprint of ordinary powder and ultrafine powder for Gardenia jasminoides f. longicarpa].

OBJECTIVE: To establish an HPLC fingerprint of Gardenia jasminoides f. longicarpa and compare the differences between its ordinary powder and ultrafine powder. METHODS: The analysis was carried out on a Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.4% phosphoric acid at the flow rate of 1.0 ml/min. The wavelength was 240 nm during 0 - 40 min and 440 nm during 40 - 80 min. RESULTS: HPLC fingerprint of Gardenia jasminoides f. longicarpa was established, 23 common peaks were identified,and the similarity of 10 samples was greater than 0.9. Ultrafine grinding did not change the types and number of chemical compositions, but it obviously increased the content of main chemical compositions. CONCLUSION: The HPLC fingerprint is accurate, reliable and repeatable, which can be used for quality control of Gardenia jasminoides f. longicarpa. Ultrafine grinding can stimulate the release of chemical components of Gardenia jasminoides f. longicarpa.

[Study on anemia among premarital women in 6 counties from 1993 to 2003].

OBJECTIVE: To describe the prevalence of anemia and yearly trends (1993-2003) among women who came to the hospitals or maternal and child health units for premarital examinations in 6 counties of Jiangsu and Zhejiang provinces. METHODS: Data were obtained from the records of the premarital examinations in perinatal health care surveillance system that had been established since 1992 in these areas. We reviewed hemoglobin levels of those women who were enrolled in the perinatal health care surveillance system from 1993 to 2003. Anemia was defined according to the WHO (2001) criterion. We calculated the prevalence of anemia and analyzed the yearly trends based on the data of hemoglobin concentration. RESULTS: In the period of 1993-2003, there were 82 995 anemia cases identified among 193,434 women with an overall anemia rate as 42.9%. The rates of anemia were high (65.5%) in 1993 but low (25.8%) in 2003. 99.7% of the anemic women whose hemoglobin concentration were between 80-119.9 g/L. Time trend analysis indicated a significant decline on anemia rate while monthly analysis showed that the prevalence rates were high (48.2%) in September and low in March (39.5%). The results also showed that the prevalence rates of anemia were relatively higher in farmers and workers in rural enterprises, and lower in Han ethnicity than minorities. The higher prevalence rates of anemia were presented among the women with less education, lower body mass index, or at older age. CONCLUSION: For those premarital women in 6 counties of Jiangsu and Zhejiang provinces, the overall anemic rate presented a significant downward trend between 1993 and 2003 while the prevalence of anemia remained high, especially for the women with less education, lower body mass index or older ages.