High-fat diet promotes prostate cancer metastasis via RPS27.

BACKGROUND: Metastasis is the leading cause of death among prostate cancer (PCa) patients. Obesity is associated with both PCa-specific and all-cause mortality. High-fat diet (HFD) is a risk factor contributing to obesity. However, the association of HFD with PCa metastasis and its underlying mechanisms are unclear. METHODS: Tumor xenografts were conducted by intrasplenic injections. The ability of migration or invasion was detected by transwell assay. The expression levels of RPS27 were detected by QRT-PCR and western blot. RESULTS: The present study verified the increase in PCa metastasis caused by HFD in mice. Bioinformatics analysis demonstrated increased RPS27 in the experimentally induced PCa in HFD mice, indicating that it is an unfavorable prognostic factor. Intrasplenic injections were used to demonstrate that RPS27 overexpression promotes, while RPS27 knockdown significantly reduces, PCa liver metastasis. Moreover, RPS27 inhibition suppresses the effects of HFD on PCa metastasis. Further mRNA sequencing analysis revealed that RPS27 promotes PCa metastasis by selectively enhancing the expression of various genes. CONCLUSION: Our findings indicate that HFD increases the risk of PCa metastasis by elevating RPS27 expression and, subsequently, the expression of genes involved in PRAD progression. Therefore, RPS27 may serve as a novel target for the diagnosis and treatment of metastatic PCa.

Spatial characteristics and influencing factors of risk perception of haze in China: The case study of publishing online comments about haze news on Sina.

Haze has become an important factor to harm people's health. In the face of the harm of haze, people express risk perception behavior through publishing online comments on haze news. To a certain extent, online comments affect the government's decision to deal with haze. However, most of the previous efforts only focused on several cities from a micro perspective whereas the existing research lacks to explore the regional differences of risk perception behavior of the whole country from a macro perspective. The macro perspective of research can help policymakers to formulate strategies of haze control according to local conditions. Therefore, it is important to understand the risk perception behavior of publishing online comments and haze from the macro perspective. In this study, we use quantitative methods to study the spatial-temporal characteristics of online comments and PM2.5 in 334 cities in China from 2014 to 2018. From the spatial perspective, the spatial characteristics of online comments are quite different from those of PM2.5 at the city level. It can be seen that the spatial mismatch can affect the government's strategy of haze control according to local conditions. From the perspective of influencing factors, we find that the risk perception behavior of publishing online comments conforms to the theory of environmental risk perception. Firstly, the local social development level is the largest driving force accelerating risk perception behavior. Secondly, information channel also has significantly positive effect on public risk perception behavior. Finally, the harmful degree of haze has the least positive effect on people's risk perception behavior. This gives us enlightenment. The number of online comments does not reflect the harmful degree of haze in different regions. We suggest that online comments should not be the main reference factor for policymakers to make strategies.

Hisian pacing restores cardiac function.

We report a 57-year-old male patient with dilated cardiomyopathy, NYHA function class III and complete left bundle branch block (CLBBB) who received cardiac resynchronization therapy using His-bundle pacing (HBP) when a left-ventricular lead could not be placed. In 10 months' follow up, his symptoms resolved rapidly, his QRS, left ventricular and left ventricular ejection fraction normalized. Recent reports support the conception that HBP may be an alternative or even better therapy for heart failure patients with CLBBB, especially if biventricular pacing is not feasible.