RESUMO
OBJECTIVE: To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis. METHODS: The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses. RESULTS: 12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map. CONCLUSION: RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.
Assuntos
DNA de Plantas/genética , Isodon/genética , Polimorfismo Genético , Técnica de Amplificação ao Acaso de DNA Polimórfico , Sequência de Bases , Primers do DNA/genética , DNA de Plantas/isolamento & purificação , Marcadores Genéticos , Isodon/classificação , Dados de Sequência Molecular , Folhas de Planta/genética , Reação em Cadeia da Polimerase , Reprodutibilidade dos Testes , Análise de Sequência de DNARESUMO
OBJECTIVE: To study the difference of rDNA ITS sequences between Cirtus grandis var. tomentosa and its relatives. METHODS: The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced. RESULTS: The DNA sequences of ITS ranged from 675 to 683 bp and the 5.8S gene of all of the samples are 165 bp in size. Distance values of ITS sequence, estimated according to Kimura two-parameter models between C. grandis var. tomentosa and C. grandis var. shatin-you, C. grandis var. tomentosa and C. reticulata were 1.05 and 2.29 respectively. CONCLUSION: There are some differences in rDNA ITS sequences among C. grandis var. tomentosa and its relatives.
