Expression and significance of the cell cycle regulators in laryngeal carcinogenesis detected by flow cytometry / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the expression and clinicopathological significance of the cell cycle regulators cyclin E, cyclin D1, p21, p16 in laryngeal carcinogenesis tissus.@*METHOD@#The expression of cell cycle regulators were detected by flow cytometry method in 23 cases of polyps of vocal cord, 69 cases of laryngeal precancerous change and 33 cases of laryngeal squamous cell carcinoma (LSCC), which tissue was paraffin embedded, sliced, dewaxed, and prepared into the cell suspension, then fluorescently labeled by cyclin E, cyclin D1, p21 and p16.@*RESULT@#In polyps of vocal cord, laryngeal precancerous change and LSCC, The positive expression rate of cyclin E and cyclin D1 were respectively 13.04%, 20.29D, 42.420 and 26.09%, 43.48% and 93.94%. The positive expression rate of p16 and p21 were respectively 61.90%, 40.98%, 14.28% and 47.62%, 23.81%, 26.23%. Those showed the positive expression rate of cyclin D1, cyclin E gradually decreased from vocal cord polyps, laryngeal precancerous change to LSCC, (P < 0.05, P < 0.01), while the positive expression rate of p21 and p16 gradually decreased (P < 0.01).@*CONCLUSION@#The abnormal expression of cell cycle regulatory factors is the molecular events of laryngeal carcinoma. High expression of positive regulatory factors cyclin D1 and cyclin E, and low expression of negative regulatory factors p16 and p21, which showed the imbalance of multiple positive and negative regulatory factors related with cell cycle play an important role in the occurrence of laryngeal cancer.

Effects of individualized nursing on the negative mood and quality of life of patients with hemoptysis / 中国实用护理杂志

Objective To study the effects of individualized nursing on the anxiety, depression mood, quality of life of patients with hemoptysis. Methods Sixty patients with hemoptysis were randomized into the intervention group and the control group with 25 cases in each. The control group received single conventional therapy while the intervention group was given individualized nursing intervention besides the conventional therapy. Using SDS, SAS and SF-36 Life Quality Measuring Scale to evaluate the effects of the patients in both groups on admission and prior to discharge. Results The scores of anxiety and depression among the intervention group were lower than those of the control group after intervention.The dimensions of quality of life besides the physical function and bodily pain in the intervention group were significantly different after intervention. Conclusions The individualized nursing intervention can improve quality of care, the state of anxiety, depression and the quality of life of patients with hemoptysis.