Toward allotetraploid cotton genome assembly: integration of a high-density molecular genetic linkage map with DNA sequence information.

BACKGROUND: Cotton is the world's most important natural textile fiber and a significant oilseed crop. Decoding cotton genomes will provide the ultimate reference and resource for research and utilization of the species. Integration of high-density genetic maps with genomic sequence information will largely accelerate the process of whole-genome assembly in cotton. RESULTS: In this paper, we update a high-density interspecific genetic linkage map of allotetraploid cultivated cotton. An additional 1,167 marker loci have been added to our previously published map of 2,247 loci. Three new marker types, InDel (insertion-deletion) and SNP (single nucleotide polymorphism) developed from gene information, and REMAP (retrotransposon-microsatellite amplified polymorphism), were used to increase map density. The updated map consists of 3,414 loci in 26 linkage groups covering 3,667.62 cM with an average inter-locus distance of 1.08 cM. Furthermore, genome-wide sequence analysis was finished using 3,324 informative sequence-based markers and publicly-available Gossypium DNA sequence information. A total of 413,113 EST and 195 BAC sequences were physically anchored and clustered by 3,324 sequence-based markers. Of these, 14,243 ESTs and 188 BACs from different species of Gossypium were clustered and specifically anchored to the high-density genetic map. A total of 2,748 candidate unigenes from 2,111 ESTs clusters and 63 BACs were mined for functional annotation and classification. The 337 ESTs/genes related to fiber quality traits were integrated with 132 previously reported cotton fiber quality quantitative trait loci, which demonstrated the important roles in fiber quality of these genes. Higher-level sequence conservation between different cotton species and between the A- and D-subgenomes in tetraploid cotton was found, indicating a common evolutionary origin for orthologous and paralogous loci in Gossypium. CONCLUSION: This study will serve as a valuable genomic resource for tetraploid cotton genome assembly, for cloning genes related to superior agronomic traits, and for further comparative genomic analyses in Gossypium.

Postnatal depression and anxiety state and its related factors of parturtents from migrant worker family / 中国实用护理杂志

Objective To know the postnatal depression and anxiety state and its related factors of parturients from migrant worker family,and then reference to certain nursing counter-measures. Methods Sampled 73 parturients by spontaneous labor from migrant worker family and 45 local matched parturients,interviewed them by SAS and SDS to know their postnatal depression and anxiety state,and then analyzed the datum between them. Results The level of postnatal depression and anxiety in parturients from migrant worker family was significant higher than that in local parturienta.Different level of depression and anxiety was existed in different parturients from migrant worker family by different educational attainments,different family type and different financial state. Conclusions The state of postnatal depression and anxiety in parturients from migrant worker family is severely,related health education should be strengthened,associated social secu-rity and support system should be established to help them release their unhealthy emotion.