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EMBO J ; 25(12): 2768-80, 2006 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-16724105

RESUMO

RNA silencing is an evolutionarily conserved system that functions as an antiviral mechanism in higher plants and insects. To counteract RNA silencing, viruses express silencing suppressors that interfere with both siRNA- and microRNA-guided silencing pathways. We used comparative in vitro and in vivo approaches to analyse the molecular mechanism of suppression by three well-studied silencing suppressors. We found that silencing suppressors p19, p21 and HC-Pro each inhibit the intermediate step of RNA silencing via binding to siRNAs, although the molecular features required for duplex siRNA binding differ among the three proteins. None of the suppressors affected the activity of preassembled RISC complexes. In contrast, each suppressor uniformly inhibited the siRNA-initiated RISC assembly pathway by preventing RNA silencing initiator complex formation.


Assuntos
MicroRNAs/metabolismo , Vírus de Plantas/fisiologia , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Animais , Arabidopsis/virologia , Pareamento de Bases , Drosophila/metabolismo , Embrião não Mamífero/metabolismo , Folhas de Planta/virologia , Estabilidade de RNA , Complexo de Inativação Induzido por RNA/metabolismo , Nicotiana/virologia , Proteínas Virais/metabolismo
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