RESUMO
The hand-net is the standard method for capturing mosquitoes with sylvatic diurnal activity in disease outbreaks in Brazil. However, occupational risks and biases related to the collectors' abilities and attractiveness are important limitations. In this study, we compared hand-nets with automatic traps (CDC) associated to CO2 and BG-Lure® in the Vassununga State Park, a Brazilian Savanna protection area. The collections carried out over 27 days on the ground and the forest canopy. A total of 1555 mosquitoes were obtained in 20 taxa. The diversity index ranged between 1.12 and 1.79 and the dominance index from 0.22 to 0.40. The dominant species on the ground was Aedes scapularis (46.0%), and in the canopy, Hg. janthinomys/capricornii (31.9%). Haemagogus leucocelaenus was rare (n = 2). The hand-net resulted in the greatest diversity and abundance of species in both strata, followed by the traps associated with CO2. A low degree of similarity was observed between the hand-net on the ground compared to the other capture methods. The use of BG-Lure® alone resulted in a low number of specimens. In conclusion, the hand-net is still the method of choice for collecting arbovirus vectors in the diurnal period, especially yellow fever vectors.
RESUMO
Avian malaria parasites are widespread parasites transmitted by Culicidae insects belonging to different genera. Even though several studies have been conducted recently, there is still a lack of information about potential vectors of Plasmodium parasites, especially in Neotropical regions. Former studies with free-living and captive animals in São Paulo Zoo showed the presence of several Plasmodium and Haemoproteus species. In 2015, a pilot study was conducted at the zoo to collect mosquitoes in order to find out (i) which species of Culicidae are present in the study area, (ii) what are their blood meal sources, and (iii) to which Plasmodium species might they be potential vectors. Mosquitoes were morphologically and molecularly identified. Blood meal source and haemosporidian DNA were identified using molecular protocols. A total of 25 Culicidae species were identified, and 6 of them were positive for Plasmodium/Haemoproteus DNA. Ten mosquito species had their source of blood meal identified, which were mainly birds, including some species that were positive for haemosporidian parasites in the former study mentioned. This study allowed us to expand the list of potential vectors of avian malaria parasites and to improve our knowledge of the evolutionary and ecological relationships between the highly diverse communities of birds, parasites, and vectors present at São Paulo Zoo.
RESUMO
Avaliou-se o hábito alimentar de Lutzomyia longipalpis em áreas de transmissão da leishmaniose visceral americana no município de Araçatuba (SP). De 1999 a 2001, capturas entomológicas mensais foram realizadas em três áreas do município, sendo pesquisadas amostras de 123 residências/área, durante 20 minutos/domicílio. As fêmeas ingurgitadas foram separadas e conservadas a -20ºC, até a realização do exame do conteúdo intestinal, pela reação de imunoprecipitação em tubo capilar. Utilizou-se anti-soros de: animais domésticos (cão, cavalo, gato e galinha), roedor e humano. Para a realização da reação, cada fêmea foi colocada em tubo com 0,2mL de solução fisiológica estéril mertiolada a 0,85%, por 12 horas. Posteriormente, foram maceradas e centrifugadas e 30ml do sobrenadante foram transferidos para o tubo capilar e adicionados 30ml de anti-soro. A reação foi dada como positiva quando observado grumo esbranquiçado até duas horas depois de seu início. Coletou-se 1.540 fêmeas de L. longipalpis, destas 699 estavam ingurgitadas quando coletadas e 613 (87,7%) foram submetidas ao teste, resultando em 45,6% de amostras reagentes. Das amostras reagentes 91,4% reagiram para sangue canino. Das 25 amostras reagentes para sangue humano 22 (88,0%) também reagiram para cão. Apenas 7,5% dos exemplares reagiram para sangue de ave. Em Araçatuba, L. longipalpis mostrou hábito eclético em relação à fonte alimentar, demonstrando alto grau de cinofilia. Embora as aves não tenham representado a principal fonte de alimento, deve-se atentar para o significado epidemiológico que elas representam, na medida em que permitem a domiciliação do vetor. Não foi observada mudança de hábito alimentar decorrente das atividades de controle vetorial.
