Characteristics of physical activity habits in type-2 diabetes patients in Spain

The aim of the study was to investigate physical activity habits in type-2 diabetes population in Spain. We surveyed a randomly stratified sample of 967 type-2 diabetes patients’ inhabitants of Spain. Physical activity levels were calculated using the IPAQ short version questionnaire. Results obtained were compared to Spanish and European general population data. Total physical activity for Spanish type-2 diabetes patients was 2267±135 MET·minuts·week. The lowest values of physical activity were found in those individuals who had the poorest glycaemic control (1765±202 MET·minuts·week in individuals with HbA1c >9% vs 2423±448 MET·minuts·week in individuals with HbA1c <7%, p< 0.05). Furthermore, sedentary individuals were more prevalent women (60%), older (66.5±1.2 vs 63.5±11 years, p= 0.003) and had higher BMI (30.3±5.6 vs 28.6±4.3 kg/m2, p= 0.011) than active individuals. Physical activity levels were higher than those reported from Spanish general (1834±122 MET·minuts·week, p< 0.05) and European populations (2058±33 MET·minuts·week p< 0.05) also, highly percentage of diabetic population walk daily compared to other groups (90% vs 83% vs 83%, p< 0.001). In conclussion, Spanish type-2 diabetes population show high levels of physical activity, greater than Spanish and European general population. This may be attributed to a higher frequency of walking as usual physical activity (AU)

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Breakfast, plasma glucose and beta-hydroxybutyrate, body mass index and academic performance in children from Extremadura, Spain.

OBJECTIVES: Nutritional aspects of breakfast, plasma levels of glucose and beta-hydroxybutyrate, body mass index and academic performance have been studied in urban and rural children (Extremadura, Spain). METHODS: Representative samples of schoolchildren (3 to 12 years old, random cluster-sampling in schools). RESULTS: Children's mean caloric intake with breakfast was 331 kcal. Rural population ingested more carbohydrates (46,9 +/- 12,3% versus 43,3 +/- 13,2% of the total caloric intake) and fewer lipids (40,5 +/- 11,8% versus 43,9 +/- 12,8% of the total caloric intake) than the urban population. Academic performance was significantly better in the children inhabiting the rural zone than in those of the urban zone. The glycaemia was higher in the urban than in the rural children, and that the contrary was the case for the beta-hydroxybutyrate values. Neither glucose nor beta-hydroxybutyrate levels were correlated with academic performance values. BMI was significantly increased in the urban versus rural children. CONCLUSION: The present results emphasize the importance of breakfast and life style in the weight and the academic performance of children.

White bean amylase inhibitor administered orally reduces glycaemia in type 2 diabetic rats.

A purified pancreatic alpha-amylase inhibitor (alpha-AI) from white beans (Phaseolus vulgaris) was administered orally (100 mg/kg body weight dissolved in 9 g NaCl/l) for 22 d to non-diabetic (ND) and type 2 diabetic (neonatal diabetes models n0-STZ and n5-STZ) male Wistar rats. Mean glycaemia (mmol/l) declined from day 4 of the alpha-AI administration in ND rats (5.48 (sem 0.08) v. 4.39 (sem 0.13); P<0.05), n0-STZ diabetic rats (7.94 (sem 0.42) v. 5.56 (sem 0.32); P<0.01) and n5-STZ diabetic rats (17.34 (sem 2.58) v. 11.93 (sem 1.96)), until the end of treatment: ND (5.22 (sem 0.21) v. 3.97 (sem 0.06); P<0.01); n0-STZ (8.10 (sem 0.19) v. 5.21 (sem 0.30); P<0.01); and n5-STZ (16.36 (sem 2.14) v. 7.69 (sem 1.34); P<0.01). There was a decrease in water intake (ml/d) in the alpha-AI-treated diabetic rats: n0-STZ (30 (sem 0.10) v. 22 (sem 1.50); P<0.01) and n5-STZ (76 (sem 5.04) v. 57 (sem 4.85); P<0.01). Food intake (g/d) decreased in all three groups: ND (23 (sem 0.31) v. 20 (sem 0.03); P<0.05); n0-STZ (22 (sem 0.55) v. 16 (sem 0.98); P<0.01); and n5-STZ (31 (sem 0.58) v. 23 (sem 1.20); P<0.01). The enterocyte sucrase and maltase activities (U/g proteins) were high (P<0.01) in the untreated diabetic rats, n0-STZ (45 (sem 4) and 152 (sem 10), respectively) and n5-STZ (67 (sem 12) and 151 (sem 10), respectively) with respect to the ND rats (24 (sem 2) and 74 (sem 10), respectively). After alpha-AI treatment, enzyme activities declined in both diabetic rats, n0-STZ (21 (sem 2) and 85 (sem 11); P<0.01) and n5-STZ (28 (sem 7) and 75 (sem 19); P<0.05), to values close to those in the ND rats. In conclusion, alpha-AI significantly reduced glycaemia in both the ND and diabetic animals and reduced the intake of food and water, and normalized the elevated disaccharidase levels of the diabetic rats.

Hypoglycaemic and anorexigenic activities of an alpha-amylase inhibitor from white kidney beans (Phaseolus vulgaris) in Wistar rats.

An inhibitor of alpha-amylase was isolated and purified from an extract of white kidney beans (Phaseolus vulgaris). The acute oral administration of the inhibitor (50 mg/kg body weight) to adult Wistar rats together with a starch load (2 g/kg body weight suspended in NaCl (9 g/l)) reduced the increase in glycaemia over the basal value (NaCl, 222 (SEM 49); inhibitor, 145 (SEM 16) mmol/l x 180 min; P<0.05) without modifying the insulin response. On administering the inhibitor orally (50 mg/kg body weight dissolved in NaCl (9 g/l)) for 21 d to rats fed on a standard diet, a decline was observed in the glycaemia values on day 0 (NaCl, 5.53 (SEM 0.12); inhibitor, 5.25 (SEM 0.16) mmol/l) relative to those obtained on days 10 (NaCl, 5.00 (SEM 0.14); inhibitor, 4.60 (SEM 0.08) mmol/l; P<0.05) and 21 (NaCl, 5.22 (SEM 0.22); inhibitor, 4.50 (SEM 0.12) mmol/l; P<0.01) of treatment, without modifying the plasma concentration of insulin. There was found to be a significant anorexigenic action of the inhibitor; there was reduced food intake (NaCl, 23.07 (SEM 0.31); inhibitor, 19.50 (SEM 0.49) g/d; P<0.01), a reduced weight gain (NaCl, 52 (SEM 3); inhibitor, -1.33 (SEM 8.9) g/21 d; P<0.01), as well as changes in the activity of some intestinal enzymes such as maltase (NaCl, 87 (SEM 7); inhibitor, 127 (SEM 11) U/g proteins; P<0.05). The present study has shown, for the first time, that the prolonged administration of an alpha-amylase inhibitor reduces blood glucose levels and body-weight gain in Wistar rats.

Morphological and enzymatic changes of the small intestine in an n0-STZ diabetes rat model.

Summary. Many studies have shown that experimental type 1 diabetes causes morphological, functional, and metabolic alterations in the small intestine. The more frequent form of the disease, type 2 diabetes, however, has been less studied. Here the influence of diabetes on the functionality of the small intestine was studied in an experimental diabetes model, with a certain degree of residual insulin secretion, specifically in the n0-STZ model. - The diabetic rats in this model were found to have glycaemia levels higher than in the controls (8.82 +/- 0.27 and 6.18 +/- 0.18 mmol/L; p < 0.01), while their plasma insulin levels were lower than in the control rats (2.65 +/- 0.32 and 3.60 +/- 0.25 ng/ml; p < 0.05). Although there were no significant variations in body weight between the two groups, both the weight and the length of the intestine were significantly greater (p < 0.05) in the diabetic rats than in the controls. The sucrase and maltase activities were greater (p < 0.01) in the proximal intestine of the diabetic rats (94 +/- 8 and 234 +/- 12 mU/mg protein, respectively) than in the control rats (50 +/- 2 and 149 +/- 20 mU/mg protein, respectively). The 6-phosphofructo-1-kinase activity (mU/mg proteins) was less (p < 0.05) in the proximal and distal intestine of the diabetic rats (160 +/- 40 and 80 +/- 20, respectively) than in the controls (280 +/- 30 and 230 +/- 30, respectively). No significant differences were observed in the lactate dehydrogenase or active and total pyruvate dehydrogenase measured in the distal and proximal intestine of control and diabetic rats. In conclusion, our results show that experimental diabetes (n0-STZ model) similar to human type 2 diabetes produces certain morphological and enzymatic alterations which affect the digestion and absorption of carbohydrates and the intestinal metabolism of glucose. These alterations may contribute to producing the post-prandial hyperglycaemia which characterizes diabetes.

Vitamins and mineral intake in elderly people from Extremadura.

To evaluate the vitamin and mineral consumption of a group of elderly people of the Comunidad of Extremadura. 122 subjects 62.5+/-5.3 years (64 women, 57 men) responded to a survey on their food intake the day before. The vitamin and mineral intake was calculated using a computer program from the University of Granada. We found no statistically significant differences by gender in the intake of the following vitamins: niacin (24.5+/-10.1 vs 25.6+/-9.7 mg/day), B1 (1.6+/-0.6 vs 1.7+/-0.6 mg/day), B6 (1.7+/-0.6 vs 1.8+/-0.5 mg/day), C (129.5+/-82.1 vs 158.1+/-97.6 mg/day), D (4.4+/-7.5 vs 3.8+/-6.5 microg/day), E (7.8+/-4.1 vs 8.1+/-3.8v mg/day), and folic acid (326.6+/-164.0 vs 383.1+/-215.3 microg/day). We did find statistically significant differences in the intake of the vitamins: A (582.2+/-299.6 vs 771.8+/-602.2 microg/day, p<0.05), B12 (23.4+/-20.3 vs 13.1+/-12.1 microg/day, p<0.001) and B2 (1.6+/-0.4 vs 1.8+/-0.5 mg/day, p<0.05). With respect to mineral intake, we found no statistically significant differences by gender in the intake of the following minerals: calcium (1046.8+/-276.9 vs 1088.9+/-307.7 mg/day), iron (20.2+/-6.6 vs 18.5+/-8.6 mg/day), iodine (73.6+/-37.9 vs 73.8+/-41.1 mg/day), potassium (2804.6+/-1088 vs 2837.5+/-1035.8 mg/day), magnesium (405.4+/-155.3 vs 366.5+/-141.3 mg/day), phosphorus (1407.4+/-408.6 vs 1317.2+/-447.2 mg/day) and zinc (8.9+/-3.3 vs 8.0+/-2.7 mg/day). We found a statistically significant difference (p<0.05) for sodium (2124.9+/-1049.5 mg/day in men and 1728.4+/-992.5 mg/day in women). Our results showed an intake less than the RDA of vit.A, while the vit.B12 intake was greater than the RDA. The intake of the other parameters fitted the recommended values.

Hypoglycaemic activity of an aqueous extract from ficus carica (fig tree) leaves in streptozotocin diabetic rats.

To investigate the hypoglycaemic activity of Ficus carica leaf aqueous extract, a decoction was administred to rats in lieu of drinking water for three weeks. The groups were: untreated non-diabetic (n = 13), untreated diabetic (n = 13), treated non-diabetic (n =13) and treated diabetic (n = 13) animals. The extract decreased (p < 0.025) plasma glucose in diabetic (27.9 ± 4.5 mmol/L to 19.6 ± 9.9 mmol/L) while not in normal rats. Plasma insulin levels were decreased by treatment (p < 0.05) in non-diabetic rats from 4.9± 1.6 ng/mL to 3.3 ± 1.2 ng/mL. Glucose uptake (µmol/min) by rat hindquarters perfused was: 5.9 ± 2.2 (untreated non-diabetic rats), 4.8 ± 2.3 (treated non-diabetic rats, p < 0.05 vs. untreated non-diabetic rats), 2.0 ± 2.0 (untreated diabetic rats, p < 0.01 vs. untreated non-diabetic rats) and 4.1 ± 3.6 (treated diabetic rats) in absence of insulin; 7.0 ± 1.7 (untreated non-diabetic rats), 8.3 ± 0.8 (treated non-diabetic rats, p < 0.05 vs. untreated non-diabetic rats), 5.0 ± 1.6 (untreated diabetic rats, p < 0.05 vs. untreated non-diabetic rats) and 6.4 ± 2.4 (treated diabetic rats) in presence of insulin. Lactate released was lower in untreated diabetic vs. untreated non-diabetic rats. Thus, Ficus carica extract showed a clear hypoglycaemic effect in diabetic rats. Such an effect cannot be mediated by an enhanced insulin secretion, so an as yet undefined insulin-like peripheral effect, may be suggested.

Hypotriglyceridaemic activity of Ficus carica leaves in experimental hypertriglyceridaemic rats.

A model of hypertriglyceridaemia in rats is described, which was used to investigate the hypolipidaemic effect of an intraperitoneal (i./p.) administration of a Ficus carica leaf decoction. Hypertriglyceridaemia was induced in rats following the protocol: a fasting period of 22 h, 2 h of oral (p.o.) administration of 20% emulsion of longchain triglycerides (LCT emulsion), both repeated once. The plasma triglyceride and total cholesterol levels obtained 2 h after the protocol were 5.7 +/- 2.5 mmol/L (p < 0.0001 vs basal levels) and 1.7 +/- 0.3 mmol/L, respectively, n = 10. The new model was used to test the hypotriglyceridaemic effect of a single dose of Ficus carica (fig tree) leaf decoction administered i./p. (50 g dry wt/kg body wt). After the i.p. injection of serum saline (control group, n = 10) or Ficus carica extract (group A, n = 10), plasma triglyceride levels in the control group and group A were 5.9 +/- 2.9 mmol/L and 5.5 +/- 2.9 mmol/L just after the LCT emulsion protocol; 4.7 +/- 2.7 mmol/L and 0.9 +/- 0.4 mmol/L, p < 0.005, 60 min after the LCT protocol; and 3.6 +/- 2.9 mmol/L and 1.0 +/- 0.4 mmol/L, p < 0.05, 90 min after the LCT protocol. The plasma total cholesterol levels, which were not modified in our experimental model, showed no significant differences in relation to baseline levels in the presence or absence of Ficus carica treatment either. The clearly positive results suggest the presence in the fig leaf decoction of a compound or compounds that influence lipid catabolism.

[Calcium intake among school children in Badajoz]. / Ingesta de calcio en escolares de Badajoz.

BACKGROUND: The intake of calcium has been studied in the diet of adolescents from a school in Badajoz (Spain) determining their BMI and their living habits (sport, consumption of tobacco and alcohol). METHODOLOGY: By means of an aleatory sampling, a sample n was selected = 207 (49.76% males and 50.24 females) with an average age of 14.14 +/- 2.08 years. They were weighed and measured to calculate their BMI. A questionnaire was carried out on consumption of products rich in calcium: milk and dairy produce, vegetables, fruit, chied fruits. The statistical analysis was carried out by means of Student t and ANOVA. RESULTS: The results was BMI < 25 (thin or standard) in 86.47%; BMI 25-30 (overweight) in 10.63% and BMI > 30 (overweight) 2.9%. The consumption of daily calcium was of 1304 +/- 702 g/ppd, higher (p < 0.001) in boys (10.45 +/- 5.51) than in girls (7.82 +/- 3.84). There is not correlation between BMI and calcium consumption. The boys observe the NIH recommendations. But girls usually don't. Milk products provide 87% of the consumed calcium. The boys who practice some sports consume 9.88 +/- 5.23 g/pps, the other ones 7.09 +/- 2.95 g/pps. CONCLUSIONS: 1. The weekly intake of calcium by surveyed students is 9.13 g/pps, smaller in girls than in boys. 2. Milk products provide 87% of the consumed calcium. 3. A 10.63% of them is overweight and a 2.9% is very overweight. 4. A 10.63% usually smokes and a 20.29% consumes alcohol. 5. A 26.27% doesn't practice any sports and consumes less calcium (p < 0.001) than the recommended quantity.

Hypoglycemic action of an oral fig-leaf decoction in type-I diabetic patients.

The effect of a decoction of fig leaves (Ficus carica), as a supplement to breakfast, on diabetes control was studied in insulin-dependent diabetes mellitus (IDDM) patients (six men, four women, age 22-38 years, body mass index (BMI): 20.8 +/- 3.0 kg/m2, HbA1c 7.6 +/- 0.9% with a mean duration of diabetes of 9 +/- 6.3 years). The patients were managed with their usual diabetes diet and their twice-daily insulin injection. During the first month, patients were given a decoction of fig leaves (FC) and during the next month a non-sweet commercial tea (TC). The patients were divided into two groups (n = 5) with random allocation and cross-over design. A standard breakfast was given at the beginning and end of each month-run. C-peptide, 2 h pre- and post-prandial glycemia, HbA1c, cholesterol, lipid fractions and hematology data, were analyzed during each visit. Glycemic profiles (7/day per week) were recorded by patients. Only two patients had intolerance dropout. Post-prandial glycemia was significantly lower during supplementation with FC 156.6 +/- 75.9 mg/dl versus TC 293.7 +/- 45.0 mg/dl (P < 0.001) without pre-prandial differences 145.0 +/- 41.5 and 196.6 +/- 43.2 mg/dl, respectively. Medium average capillary profiles were also lower in the two sub-groups of patients during FC 166.7 +/- 23.6 mg/dl, P < 0.05 and 157.1 +/- 17.0 mg/dl versus TC 245.8 +/- 14.2 mg/dl and 221.4 +/- 27.3 mg/dl. Average insulin dose was 12% lower during FC in the total group. The addition of FC to diet in IDDM could be of help to control postprandial glycemia.

Effect of miglitol administration to non-insulin-dependent diabetic rats.

1. The effect of the acute or chronic oral administration of miglitol (Bay M 1099 alpha-glucosidase inhibitor) to non-insulin-dependent diabetic rats was studied. 2. The acute oral administration of miglitol (10 mg/kg b.w.) reduced significantly the increment of blood glucose after oral maltose (2 g/kg b.w.) overload (364 +/- 58 and 205 +/- 12 mmol/90 min, without and with miglitol respectively; P < 0.05). 3. Under chronic oral administration of miglitol (10 mg/kg b.w.), two days after the start of treatment the blood glucose dropped from 7.53 +/- 9.59 to 4.40 +/- 5.50 mmol/l. The plasma insulin, cholesterol, or triglycerides levels were not modified. 4. A significant reduction (P < 0.01) in water and food intake was observed. Normal rats values were not affected by miglitol treatment.

Individual housing influences certain biochemical parameters in the rat.

Individual housing has been reported to modify animal behaviour. The present study compares the plasma levels of glucose, triglycerides and total cholesterol, weight, and food and water intake in two groups of female rats. Group A: 10 rats who remained grouped in two cages for 21 days; and Group B: 10 rats housed in two cages for 7 days, then isolated in individual cages from day 8 to day 15, and finally grouped together again for the last 7 days of the study. The results showed that the plasma values of glucose declined (P < 0.05) in the Group B rats when they had been returned to group condition (4.79 +/- 0.72 mM) than when they had been isolated (5.45 +/- 0.94 mM). Plasma triglyceride levels were lower (P < 0.05) in isolated rats (0.70 +/- 0.26 mM) than in any determination of the grouped rats. Group B: 1st week 1.21 +/- 0.21 mM, 3rd week 0.88 +/- 0.20 mM; and Group A: 1.22 +/- 0.20, 0.96 +/- 0.16, and 0.96 +/- 0.36 mM, in the first, second, and third week, respectively. There were no statistically significant differences in total cholesterol values as a function of the individual housing of animals. While there was no weight difference between the two groups of rats that could be ascribed to individual housing, there was a statistically significant increase (P < 0.05) in the food intake of isolated rats (17.5 +/- 3.2 g) with respect to values in the same Group B animals when they were housed together (1st week, 16.6 +/- 2.8 g; 3rd week, 16.8 +/- 3.1 g). These results therefore confirm that individual housing of female rats provoke variations in certain biochemical parameters, and that if this is not taken into account in performing different scientific studies, it could give rise to unreliable or even dubious results.

Mesenteric arteriovenous differences in glucose, lactate and insulin concentrations in rats and humans.

The present study was aimed to investigate the mesenteric arteriovenous differences in blood glucose and lactate and plasma insulin in humans (n=8) and rats (n=10). Arterial (abdominal aorta) and mesenteric vein blood glucose and lactate (enzymatic methods) and plasma insulin concentrations (radioimmunoassay) were measured in patients during abdominal surgery and in normal rats. Blood glucose levels were significantly (p < 0.05) higher in the abdominal aorta than in the mesenteric vein in both rats (9.2+/-1.0 vs 7.5+/-0.8 mmol/l) and humans (10.4+/-2.9 vs 8.5+/-2.7 mmol/l). Blood lactate levels were higher (p < 0.05) in the mesenteric vein in both rats (3.7+/-1.3 vs 2.8+/-0.9 mmol/l) and humans (0.7+/-0.23 vs 0.1+/-0.05 mmol/l). Plasma insulin concentrations were identical in the aorta or mesenteric vein in both rats (314.4+/-162.0 vs 311.4+/-94.2 pmol/l) and humans (62.4+/-43.2 vs 61.8+/-48.0 pmol/l). In conclusion, both rat and human intestine retained a high proportion of arterially administered glucose and released lactate under the studied conditions.

In vitro study of the effect of miglitol on carbohydrate digestion and intestinal metabolism in normal and non-insulin-dependent diabetic rats.

The effect of miglitol was studied (20 mg/kg body weight), administered intraduodenally alone or together with maltose, on the absorption and intestinal metabolism of glucose during its translocation from the lumen of the intestine to the blood, using in vitro perfused preparations of complete small intestine-pancreas, proximal small intestine alone, or distal small intestine alone, isolated from normal and non-insulin-dependent diabetic rats. In the absence of a luminal administration of maltose in normal rats, the glucose uptake from the vascular perfusate was greater in the presence (0.52 +/- 0.04 mmol/h) than in the absence (0.39 +/- 0.02 mmol/h) of miglitol (p < 0.05). In diabetic rats, no significant variations were observed in glucose uptake from the vascular perfusate as an effect of miglitol, but the glucose uptake in the presence of this drug was significantly less (p < 0.05) than that observed in normal rats. Portal lactate was significantly greater (p < 0.05) in diabetic than in normal rats and, after administration of miglitol, rose in both normal and diabetic rats, the rise being significantly greater in normal than in diabetic rats (p < 0.01). When maltose was administered luminally (2 g/kg body weight), the values of portal glucose in both normal and diabetic rats were significantly less in the presence of miglitol in the complete as well as in the distal and proximal small intestine preparations (p < 0.05); the glucose uptake from luminal administered maltose was greater in the presence of miglitol in diabetic (p < 0.05) and in normal (p < 0.05) rats except in the complete small intestine of normal rats; and no significant differences were observed in portal lactate levels between normal and diabetic rats in the presence of miglitol. In conclusion, our results show that miglitol administered luminally at the doses employed here, as well as reducing the transport of glucose from the lumen of the intestine into the blood supply, significantly stimulate intestinal glucose metabolism.

Changes in plasma enzyme activities in professional racing cyclists.

OBJECTIVE: To reports changes in plasma levels in professional racing cyclists. METHODS: Plasma levels of the intracellular enzymes aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transpeptidase, lactate dehydrogenase, and alkaline phosphatase were measured resting and after exercise in professional cyclists participating in two road races: Vuelta Ciclista a Valencia (800 km, beginning of the cyclist season), and the top rank race Vuelta a España (2700 km, at the end of cyclist season). RESULTS: A significant increase in plasma aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase was observed at the end of the race over the corresponding paired start values (P < 0.05). The increase in plasma lactate dehydrogenase after the race was only statistically significant (P < 0.05) in the Vuelta a España. The longer the duration of the cycle tour race, the greater were the increases in aspartate aminotransferase and alanine aminotransferase levels. The high levels returned to their start values during the overnight resting period. CONCLUSIONS: The presence of these enzymes in the blood is probably due to mechanically damaged muscle cells leaking their contents into the interstitial fluid.

Experimental streptozotocin-reduced diabetes and intestinal glucose metabolism in the rat, in vivo and in vitro.

The intestine has a high glycolytic activity, but its metabolic role could be altered in diabetes mellitus. The aim of the present work was to investigate in vivo the glucose retained and the lactate produced by the intestine of normal and diabetic rats and in vitro the effect of different arterial glucose concentrations on glucose utilization and lactate, alanine, and pyruvate production in normal and diabetic rats when the glucose is supplied to the intestine exclusively via the vascular route. In vivo, the normal and diabetic rats retained similar percentages of the arterially supplied glucose (14.7 +/- 2.4, respectively). In vitro, when the preparations were perfused under hyperglycemic conditions, the glucose consumed, as a fraction of the quantity infused, was significantly lower (P < 0.05) in the diabetic (247.0 +/- 22.8 mumol/mmol infused glucose) than in normal (315 +/- 16.3 mumol/mmol infused glucose rats) rats. The lactate produced was significantly higher in diabetic than in normal rats whether the preparations were perfused under isoglycemic (P < 0.01; 1916.4 +/- 124.0 vs vs 1284 +/- 67.7 mumol/mmol consumed glucose) or hyperglycemic (P < 0.05; 1356.4 +/- 199.7 vs 898.0 +/- 87.3 mumol/mmol consumed glucose) conditions. There was significantly (P < 0.05) greater alanine release from the diabetic (123.7 +/- 21.8 mumol/mmol consumed glucose) than from the normal (40.7 +/- 10.3 mumol/mmol consumed glucose) rat preparations perfused under isoglycemic conditions.

[A descriptive clinical study of a type of arthritis in beekeepers of the Badajoz area of La Siberia Extremeña]. / Estudio clínico descriptivo de un tipo de artritis en apicultores de la comarca pacense de la Siberia Extremeña.

BACKGROUND: The appearance of episodes of arthritis has been detected in beekeepers in the Siberia Extremadura (Spain) related to working with the hives. This present work describes the clinical features of such arthritic syndrome. METHODS: Sixty cases were selected at random from a previous epidemiological study to undergo a clinical protocol that included, anamnesis, physical signs, haematological, biochemical and immunological analyses, and radiological exploration of hands, wrists, feet, and pelvis. RESULTS: The picture is characterized by episodes of oligoarthritis associated with bee-stings in the affected joints or nearby. The most frequent radiologic lesions are pinched articular lines, sclerosis, and the presence of geodes. Analytically, there was frequent eosinophilia, abnormalities in haemostasis tests, and a rise in serum alkaline phosphatase. CONCLUSIONS: An acute inflammatory oligoarthritis of unknown cause has been described which affects the hands asymmetrically, and which is found in beekeepers in relation to their work with the hives. It occasionally involves into a chronic localized arthropathy capable of provoking ankylosis and permanent articular disability.

HDL-cholesterol increase associated to triglycerides degradation in vitro.

The effect of muscle tissue from rats trained by swimming on the extracellular degradation of triglyceride (TG) rich particles has been studied in vitro. During incubation, there is a progressive decline of the TG concentration in the incubation medium. At the end of the incubation period (90 min), a significantly reduction in the TG levels (p < 0.05) is associated with a significant increase in the high-density lipoprotein (HDL) cholesterol level (p < 0.005). There are no significant changes in total cholesterol levels. The correlation of the TG decline with the HDL-cholesterol increase is significant (r = 0.695, p < 0.05, n = 25). The experimental model used here could be of great interest for the in vitro study of factors affecting lipid levels in plasma.

Age-related increased 14C-arachidonic acid uptake by platelets in normal subjects.

Arachidonic acid uptake activity (pmol/10(8) platelets min) measured in platelets obtained from normal subjects was significantly higher in over-forty year old (3.53 +/- 0.38) than in under-forty year old subjects (2.33 +/- 0.12). No significant correlations were found between the arachidonic acid uptake activity and fasting plasma glucose, total cholesterol, HDL-cholesterol or triglyceride levels. The arachidonic acid uptake activity was significantly reduced by the presence of indomethacin in platelets obtained from both under and over-forty year old subjects, and by the presence of nordihydroguaiaretic acid in platelets obtained from over-forty year old subjects. In conclusion, these data show that arachidonate uptake activity by platelets increased with age. This increase was abolished when platelets were incubated in the presence of inhibitors of the arachidonic acid metabolism.