Anti-inflammatory effects of ischemic preconditioning on rat small bowel allografts.

INTRODUCTION: Minimizing the inflammatory events that follow intestinal transplantation may influence immediate graft function and improve outcome. Ischemic preconditioning (IPc) has been shown to ameliorate early inflammatory responses, and it may also attenuate the potentially damaging inflammation after intestinal transplantation. Herein, we examine the influence of intestinal IPc on inflammatory indices (tissue expression of ICAM-1, CD11a, and CD44 and serum levels of the soluble ICAM-1, sICAM-1) after heterotopic intestinal transplantation. METHODS: Lewis rats received full-length preconditioned or non-preconditioned Brown Norway intestinal allografts in the absence of immunosuppression. Preconditioned grafts were subjected to 1 cycle of 10 minutes of ischemia-reperfusion. Preconditioned and non-preconditioned isografts acted as controls. Blood was collected on alternate days post-transplant, and graft tissue harvested on sacrifice. ICAM-1, CD44, and CD11a expression was determined by immunohistochemistry, and the area of staining was quantified using image analysis. Serum soluble ICAM-1 levels were determined using an R&D Systems Quantikine enzyme immunoassay. RESULTS: (1) IPc ameliorated serum levels of sICAM-1 until severe rejection (day 7) overcame this down-regulation when compared to non-preconditioned allografts (day 3: 34,304 vs 40,479 pg/mL; day 5: 52,441 vs 61,593 pg/mL; day 7: 75,114 vs 73,309 pg/mL; day 9: 72,872 vs 76,314 pg/mL, respectively). (2) ICAM-1 expression was significantly lower in preconditioned allografts (1.02 vs 2.01 mm(2)). (3) CD44 tissue levels were also found to be lower in preconditioned allografts (0.86 vs 1.13 mm(2)). (4) There was a significant relationship between tissue ICAM-1 expression and serum levels of soluble ICAM-1 (P < .02). CONCLUSIONS: IPc improves inflammatory indices in the early stages following intestinal transplantation, and this might lead to a preserved cellular, architectural, and functional graft status. Furthermore, our results support the use of soluble ICAM-1 as a marker of endothelial activation, and thence of inflammation and developing rejection.

CMV colitis presenting as acute abdomen requires early diagnosis and treatment to avoid mortality.

OBJECTIVE: Acute CMV-colitis is an unusual cause of emergency colectomy in a general surgical department. The aim of this article is to report our experience on CMV colitis as a cause of acute abdomen. METHODS: Five patients were referred to general surgery during a three-year period with acute abdomen due to gastrointestinal CMV disease. All patients underwent serology for CMV except case 5. In all patients a gastroscopy or colonoscopy or both, based on individual clinical symptoms, was performed. Abdominal CT scan was requested in patients 1 and 2. Antiviral therapy was instituted in all patients. One patient (case 5) underwent surgery without prior diagnosis of CMV. RESULTS: There were four men and a woman with a median age of 56 years. Abdominal CT revealed perforation in one patient, later confirmed at laparotomy. Four patients had a positive CMV diagnosis either on serology or tissue biopsy or both. Three patients were treated surgically of whom two died of multiorgan failure. CONCLUSIONS: CMV may behave in the intestine as a non-pathogenic bystander. However, any condition that compromises the immune status may be sufficient to trigger its virulence. Severe colitis commits clinicians to start or increase immunosuppression regimens, whereas in gastrointestinal CMV disease such an approach inevitably aggravates the clinical picture. Tissue biopsy remains the gold standard test in CMV colitis. However, a positive diagnosis by 'any means' should prompt early antiviral treatment. Surgery should be limited to acute complications of CMV colitis and to unresponsive disease.

Colocutaneous fistula complicating therapeutic mesenteric embolisation.

Percutaneous embolotherapy has now assumed an important role in the management of massive colonic haemorrhage. However, this therapeutic option is associated with a significant risk of irreversible segmental colonic ischaemia. We present a case where distal segmental ischaemia led to a colocutaneous fistula, a complication not reported so far in the literature.

An experimental technique to assess the immunologic consequences of segmental small bowel transplantation.

BACKGROUND: The amount of native small bowel required for adequate nutrition is variable, but lies between 10% and 20% of full length. Currently, for patients requiring small bowel transplantation (SBT), standard practice is to transplant the entire small bowel if space permits. Few experimental studies have addressed the effect of the length of small bowel transplanted on immune responses and in those that have, the amount of mesenteric lymph node (MLN) transplanted has always been a potential confounding factor, as have differences between jejunum and ileum. METHODS: Full-length and segmental heterotopic rat SBT was performed between PVG donor and DA recipients. To transplant reduced length small bowel grafts but to exclude immunologic differences between jejunum and ileum, equal lengths of bowel were resected from proximal and distal ends in the donor. A proportional amount of MLN was carefully dissected using a microvascular technique and then excised. Serial serum samples from the transplant recipients were tested for anti-PVG (rejection) and anti-DA (graft-versus-host) antibodies using a two-color flow cytometric technique, described previously, with the aim of looking for differences in immunologic responses to full and segmental grafts. RESULTS: We have established a model of segmental SBT that includes a proportional amount of MLN and is free from differences between jejunum and ileum. Preliminary data have demonstrated the development of circulating anti-host and anti-graft antibodies with time for both full-length and segmental SBT.

Perioperative analgesia in experimental small bowel transplantation.

BACKGROUND: Despite numerous studies in experimental rat small bowel transplantation (SBT), few authors make reference to perioperative analgesia. Recent changes to the Animals (Scientific Procedures) Act 1986 in the United Kingdom have made the use of analgesia in laboratory animals compulsory because pain is unnecessary in the majority of scientific procedures. METHODS: Heterotopic SBT (PVG-->DA) was performed on male rat recipients weighing 220 to 250 mg under isoflurane with a mean anesthetic time of 100 minutes. Recovery from anesthesia was usually within 15 minutes. Analgesia regimens were based on those in common use for other procedures. All drugs were administered in the 30 minutes prior to recovery from anesthesia. Group A received carprofen (2 mg/kg IM or SC). Group B was given buprenorphine (0.05 mg/kg either IM or SC). Group C received paracetamol (10- 30 mg) rectally. An early postoperative scoring system of four criteria was used, giving a maximum (least desirable) score of 16. Sixty transplants were performed, divided between the three groups. RESULTS: In group A animals scored a median of 1 of 16 but all except three recipients died within 3 hours. Those in group B scored a median of 8 of 16, but all animals except one died between 4 to 16 hours after surgery. Group C had a median score of 11 of 16, but there was no early mortality. Postmortem examination excluded technical failures in all but three animals. CONCLUSION: We recommend the use of paracetamol for perioperative analgesia in SBT because of the high mortality associated with other drugs when used in this procedure.

Endoscopic sympathectomy in the treatment of facial blushing.

INTRODUCTION: Bilateral endoscopic thoracic sympathectomy (BETS) has been shown to be an effective, permanent, and safe treatment for severe upper limb hyperhydrosis. More recently, the possibility of using BETS to treat facial blushing, a redness of the face bought on by emotional or social stress, has been raised. This followed incidental reports from patients of relief from their blushing following this procedure for hyperhydrosis. At King's College Hospital, 120 patients underwent BETS over a 3-year period for both upper limb hyperhydrosis and facial blushing. In this study we report our results in relation to facial blushing. PATIENTS AND METHODS: The outcome was evaluated by questionnaire and symptoms assessed using the visual analogue scale. Questions on postoperative complications and overall quality of life were included. RESULTS: A total of 80 patients responded to our questionnaire of whom 59 (74%) experienced facial blushing. In 12 patients, this was their only symptom. Severity of facial blushing was reduced from a mean score of 78 before operation to 26 after BETS (P < 0.001); 29% reported complete resolution of their facial blushing. There was no mortality or conversion to open surgery. Quality of life was reported to be much better in 63% of facial blushers following the procedure. CONCLUSIONS: This study demonstrates both a statistically significant reduction in severity of facial blushing as well as a clear improvement in quality of life following a safe procedure with few complication rates. Facial blushing can, therefore, be considered as an indication for BETS on its own merit when not associated with hyperhydrosis.

Abdominal complications from crack cocaine.

Not only is cocaine a powerfully addictive and dangerous drug of abuse, the use of the purified cocaine derivative crack has also reached epidemic proportions. Apart from causing fatal cardiorespiratory complications, crack cocaine is capable of producing surgical emergencies, which may or may not be associated with the pharmacology of cocaine itself. This is a report of crack-induced pneumoperitoneum, the mechanism of which seemed to be related to the prolonged Valsalva manoeuvre during crack smoking. Other differential diagnoses of crack related pneumoperitoneum are also discussed.

Current status in the management of proximal bile duct carcinoma.

The confluence of the biliary duct constitutes the most common location of the bile duct neoplasms. Resection of the tumor is the only procedure which has met with long-term survival rates (more than 5 years). An enhanced exposure of the tumor in surgical intervention contributes to increasing the number of resectable cases. The transhepatic approach through the principal incision offers the best possibility to explore the tumors of the proximal bile duct confluence, using this approach the resection rate is higher than that of other routes. The surgical management of confluence biliary duct carcinoma can be curative if the diagnosis is made in early stages of the disease and if at that time resection is possible.

Use of the renal pelvis as exocrine drainage in segmental pancreatic transplantation. A preliminary report.

A new technique for exocrine drainage in segmental pancreatic transplantation has been developed in which the renal pelvis is anastomosed to the pancreatic capsule in an end-to-end fashion, after nephrectomy. This technique can be used only in uraemic patients without renal function. The procedure was performed on a uraemic patient who was free of major problems until his death due to an intracranial haemorrhage, 41 days after transplantation. The procedure described here would appear as a new form of drainage of the pancreatic secretions in segmental grafting, with advantages over techniques previously described.

Successful long-term results with resection of pancreatic carcinoma in children: favorable prognosis for an uncommon neoplasm.

Carcinoma of the pancreas is increasing in incidence and affecting a younger population. However, pancreatic carcinoma in children under 15 years of age is a rarity. Only 28 cases were reported in the literature from 1885 to 1981. All of the known patients who have undergone resection of the tumor are alive at the present time. The differential diagnosis of abdominal masses has been further elucidated by abdominal tomography. Pancreaticoduodenectomy or distal pancreatic resections may be fitted to the individual case with reasonable expectations of patient survival.

Changes in circulating glycerol, free fatty acids and glucose levels following liver transplant in the pig.

Female pigs, fasted overnight, received an orthotopic liver transplant. During the nonhepatic phase, both blood glycerol and plasma free fatty acid concentrations increased, returning to basal values after the transplant, indicating that the liver is the main receptor of these products released in the blood from the glyceride breakdown in peripheral fat deposits. Blood glucose level rose during the nonhepatic phase, probably due in part to the perfusion of glucosated saline received by the animals during this phase. After liver transplant, blood glucose levels progressively decreased and this effect was greatly reduced by administering L-alanine. Our data indicate that metabolic changes in the donor's liver diminish the availability of gluconeogenetic substrates immediately following transplant, while administration of exogenous alanine permits faster restoration of gluconeogenetic function in the transplanted liver.

Giant diverticula of the colon.

A case of a gas-filled intra-abdominal abscess cavity is reported. The literature is reviewed for similar cases and with reference to the cause and differential diagnosis of abdominal air cysts.

Intrahepatic vascular division in the pig: basis for partial hepatectomies.

Transplantation of resected hepatic fragments, with the attendant vascular and biliary tract reconstruction, presents difficulties. We have studied the intraparenchymal vascular division in the pig with a view to performing partial hepatectomies with the greastest possible anatomical support. Forty-six molds of the various vascular territories were obtained from the 31 porcine livers. Each segment of the median lobe is dependent on the adjacent lateral lobe. Its intersegmental fissure is the obligatory site for parenchymal section to be continued through horizontal portion of portal vein, hepatic artery, and left hepatic duct. A right hemihepatectomy unfailingly results in the devitalization of the left side of the liver. If the goal of a hepatectomy is the transplantation of the resected fragment, it is recommended that the right side of the liver be utilized, as its more convenient extrahepatic vascular and biliary calibre will permit pedicular conservation and anastomosis.