Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Res Vet Sci ; 124: 263-269, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31003008

RESUMO

Porcine reproductive and respiratory syndrome virus (PRRSV) is known to be clinically responsible for reproductive failure in sows and post-weaning respiratory disease in growing piglets. During the last years, highly pathogenic PRRSV isolates have been discovered. In Italy, a PRRSV-1 subtype 1 strain (namely PR40/2014) characterized by high pathogenicity was isolated and experimental infection was characterized in terms of virological/clinical features and immune modulation (Canelli et al., 2017; Ferrari et al., 2018). The present study was performed in 4-week-old pigs experimentally infected with the highly pathogenic PRRSV1_PR40/2014 (HP-PR40) or with the conventional PRRSV1_PR11/2014 (PR11). The aim was to evaluate the interrelation between plasmatic hormones and cytokines in infected pigs compared to uninfected controls in order to address potential effects on the course of an experimental infection. The time-related changes of growth hormone (GH), insulin-like growth factor-1 (IGF-1), adiponectin, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels appear to be modulated by the infection depending on the PRRSV isolate (HP-PR40 vs. PR11). In particular, in HP-PR40 infected animals, the association between high GH levels and viremia may testify the need to block the anabolic action of GH in order to shift available energy towards the immune response. This need appeared to be delayed in PR11 animals, given the lower pathogenicity of the isolate. Adiponectin, IL-6 and TNF-α course supports the hypothesis of GH resistance mechanisms to guarantee homeostasis in HP-PR40 animals and underlines the key role of energy availability in events leading to an effective response to the virus.


Assuntos
Citocinas/sangue , Hormônios/sangue , Síndrome Respiratória e Reprodutiva Suína/metabolismo , Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia , Animais , Feminino , Masculino , Síndrome Respiratória e Reprodutiva Suína/fisiopatologia , Suínos
4.
Transbound Emerg Dis ; 62(2): 217-22, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23865469

RESUMO

Hepatitis E virus (HEV) is responsible for sporadic acute hepatitis in developed countries, where the infection is acquired probably through ingestion of contaminated food, in addition to travel-related cases. In this study, the circulation of HEV in wild boar from nine Italian provinces was evaluated. An overall seroprevalence of 10.2% was found, although there were differences among the provinces, while no samples were positive for HEV RNA detection. This study indicates an active circulation of HEV in the Italian wild boar populations and suggests to consider the zoonotic risk in handling and eating meat from this animal.


Assuntos
Vírus da Hepatite E/genética , Hepatite E/veterinária , Carne/virologia , Sus scrofa/virologia , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Animais , Hepatite E/epidemiologia , Itália/epidemiologia , Modelos Lineares , Reação em Cadeia da Polimerase/veterinária , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Estudos Soroepidemiológicos , Suínos
5.
Zoonoses Public Health ; 60(1): 84-92, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22931153

RESUMO

Summary This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT-PCR assays targeting the L1 gene. Through a multiplex RT-PCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference strains obtained from GenBank, belonging to the three serotypes. Molecular analysis of the S1 gene revealed that the amino acid residues associated with neurotropism (198-204NLAIRLP, 249I, 350D and 419E) were highly conserved among the Italian bat strains. These results suggest that potentially neurotropic MRV type 3 strains are widespread among Italian bats. Furthermore, the identification of MRV type 3 in bat species such as Pipistrellus Khulii, which is common in urban areas and known for its close contact with humans, underlines the need for vigilance.


Assuntos
Quirópteros/virologia , Orthoreovirus de Mamíferos/isolamento & purificação , Infecções por Reoviridae/veterinária , Sequência de Aminoácidos , Animais , Sequência de Bases , Linhagem Celular , Chlorocebus aethiops , Fezes/virologia , Humanos , Itália/epidemiologia , Dados de Sequência Molecular , Orthoreovirus de Mamíferos/classificação , Orthoreovirus de Mamíferos/genética , Filogenia , RNA Viral/genética , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/virologia , Alinhamento de Sequência , Análise de Sequência de DNA , Células Vero , Tropismo Viral
6.
Transbound Emerg Dis ; 57(6): 434-42, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21040508

RESUMO

Porcine enterovirus (PEV), Porcine Teschovirus and Porcine sapelovirus, belonging to the family Picornaviridae, are ubiquitous and mainly cause asymptomatic infections in pigs. In this study, a total of 40 Italian porcine picornavirus isolates were characterized by sequencing the capsid VP1-encoding gene. This procedure turned out to be a useful diagnostic tool for the molecular identification of porcine enterovirus, teschovirus and sapelovirus strains and for the study of molecular epidemiology and evolution of these viruses confirming the possibility of correlating virus genotype to serotype.


Assuntos
Proteínas do Capsídeo/genética , Infecções por Picornaviridae/veterinária , Picornaviridae/classificação , Picornaviridae/genética , Doenças dos Suínos/diagnóstico , Animais , Biomarcadores , Primers do DNA , Bases de Dados de Ácidos Nucleicos , Itália , Filogenia , Picornaviridae/isolamento & purificação , Infecções por Picornaviridae/diagnóstico , Infecções por Picornaviridae/virologia , Reação em Cadeia da Polimerase/veterinária , Análise de Sequência de DNA/veterinária , Suínos , Doenças dos Suínos/virologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...