RESUMO
Fruit's decoctions of Passiflora edulis and P. foetida var. albiflora were evaluated for the inhibition of activity of gelatinase MMP-2 and MMP-9, two metallo-proteases involved in the tumour invasion, metastasis and angiogenesis. Both water extracts, at different concentrations, inhibited the enzymes.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Inibidores de Metaloproteinases de Matriz , Passiflora , Fitoterapia , Extratos Vegetais/farmacologia , Inibidores Teciduais de Metaloproteinases/farmacologia , Antineoplásicos Fitogênicos/administração & dosagem , Antineoplásicos Fitogênicos/uso terapêutico , Relação Dose-Resposta a Droga , Frutas , Humanos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Inibidores Teciduais de Metaloproteinases/administração & dosagem , Inibidores Teciduais de Metaloproteinases/uso terapêuticoRESUMO
A stable cell suspension line of Catharanthus roseus producing anthocyanin was obtained. In this strain it was found that approximately 30% of cells regularly accumulated these metabolites and that anthocyanin accumulation occurred between the second half of log phase and the stationary phase of the culture growth cycle. The anthocyanins in the suspension cultures were compared with those biosynthetized in the flowers both of regenerated by somatic embryogenesis and field-grown plants. Six anthocyanins were identified in all the examined samples, three 3-O-glucosides and three 3-O-(6-O-p-coumaroyl) glucosides of petunidin, malvidin and hirsutidin. The hirsutidin coumaroyl glucoside has not been reported previously, and was predominat in all samples. The anthocyanin relative content was similar for cell suspensions and flowers from regenerated plants but different from field-grown plant flowers; instead, the total content was almost the same for the two flower types and higher compared to suspension culture content.
Assuntos
Antocianinas/biossíntese , Catharanthus/química , Catharanthus/crescimento & desenvolvimento , Fitoterapia , Extratos Vegetais/química , Antocianinas/química , Catharanthus/citologia , Catharanthus/fisiologia , Linhagem Celular , Células Cultivadas , Flores , Humanos , Espectrometria de Massas , RegeneraçãoRESUMO
A protocol for shoot induction from callus of Haplophyllum patavinum was established. Two known furoquinoline (skimmianine and haplopine), and three quinolone (edulinine, ribalinine and isoplatydesmine) alkaloids were isolated for the first time from plant material, callus and shoot cultures of this species. The structures of these compounds have been characterised on the basis of spectroscopic evidence.
Assuntos
Alcaloides/isolamento & purificação , Compostos Heterocíclicos com 3 Anéis/isolamento & purificação , Plantas Medicinais/química , Quinolinas/isolamento & purificação , Quinolonas/isolamento & purificação , Rutaceae/química , Alcaloides/química , Técnicas de Cultura , Compostos Heterocíclicos com 3 Anéis/química , Itália , Espectrometria de Massas , Estrutura Molecular , Quinolinas/química , Quinolonas/química , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
A semiquantitative determination of two bis-indole antitumor alkaloids, vincristine and vinblastine, has been performed by flow-injection electrospray ionization mass spectrometric analysis of the extracts of Catharanthus roseus. Leaves and flowers of two different phenotypes (pink flower and white flower) obtained from somatic embryogenesis were thus examined and compared with the field-grown mother plant. Different amounts of vincristine and vinblastine were detected depending on the examined samples.
Assuntos
Antineoplásicos Fitogênicos/análise , Plantas Medicinais/química , Vimblastina/análise , Vincristina/análise , Antineoplásicos Fitogênicos/biossíntese , Calibragem , Análise de Injeção de Fluxo , Folhas de Planta/química , Folhas de Planta/metabolismo , Plantas Medicinais/embriologia , Plantas Medicinais/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Vimblastina/biossíntese , Vincristina/biossínteseRESUMO
The utilization of more than 90 plant species as folk external remedies to treat rheumatic and arthritic diseases is reported, and their possible effectiveness is discussed according to present phytochemical and pharmacological knowledge. Some of the plant species used exhibit skin-irritant properties (protoanemonin, isothiocyanate or allicine yielding plants and species containing capsaicin, oleoresins, volatile oils, resinous principles, alkaloids). The effectiveness of other herbal remedies depends on their content of salicyl derivatives. A considerable number of the plant species utilized contain chemical products (triterpenoid or steroid compounds, alkaloids) for which anti-inflammatory properties have been demonstrated.