The impact of amphetamine-related disorders on community psychiatric emergency services: 1993-1997.

Trends in number, relative prevalence, and demographics of adult psychiatric admissions to the county psychiatric emergency service in Sacramento, California, for treatment of Amphetamine-Related Disorders (ARDs) (N = 2,428) between July 1993 and July 1997 were examined. ARDs accounted for 11.2% of all emergency admissions (N = 21,615) and were the most frequent substance-related disorder requiring admission during the study period. Patients with ARDs tended to be Caucasian or Hispanic males in their mid 30s. 38% of the ARD patients required further inpatient psychiatric care. The trends in numbers of ARDs tended to parallel other local and national non-psychiatric indicators of amphetamine-induced morbidity.

C2H2-546: a zinc finger protein differentially expressed in HTLV-1 infected T cells.

We report the cloning and characterization of a novel cDNA termed C2H2-546 which encodes a C2H2-type zinc finger protein. C2H2-546 RNA is expressed in the HTLV-1 infected T cells examined which were derived from HAM-TSP patients, but not in T cells derived from ATL patients. The C2H2-546 gene is conserved in humans and primates and maps to chromosome 10q11.2, a site associated with a variety of cancers. Thus, C2H2-546 is a candidate regulatory molecule important in the formation of these tumors, and may serve as an important marker to distinguish HTLV-1 infected ATL versus HAM-TSP T cell lineages.

C2H2-171: a novel human cDNA representing a developmentally regulated POZ domain/zinc finger protein preferentially expressed in brain.

We describe a novel human zinc finger cNDA. C2H2-171. This cDNA represents an mRNA which encodes a protein of 484 amino acids and a calculated molecular weight of 54 kD. Four zinc finger-like domains are found in the C-terminal end of the protein. At the N-terminus, C2H2-171 contains a POZ/tramtrack-like domain similar to that found in the tumor associated zinc finger proteins LAZ-3/BCL-6 and PLZ-F, as well as in non-zinc finger proteins. C2H2-171 RNA is preferentially expressed in the brain, and increases during the course of murine development, with maximal expression in the adult. C2H2-171 RNA is differentially expressed in brain regions, with the highest level of expression in the cerebellum. C2H2-171 RNA was expressed at high levels in primary cerebellar granule cell neurons compared to astrocytes. The gene encoding C2H2-171 is highly conserved in vertebrates, and maps to the terminus of human chromosome 1 (1q44-ter). This chromosomal location is associated with a number of cytogenetic aberrations including those involving brain developmental anomalies and tumorigenesis. These data suggest that C2H2-171 may play an important role in vertebrate brain development and function.

Factors predicting distress among caregivers to children with chronic medical conditions.

Explored informant differences and assessed the importance of sociodemographic factors, child health status characteristics, and caregiver burden to variability in psychological symptoms among caregivers to 116 children with chronic medical conditions. Important informant effects were found. Caregiver-reported, but not physician-reported, burden was an important predictor. Severity of illness, whether rated by physician or caregiver, had little predictive effect whereas child impairment predicted greater distress, independent of sociodemographics. Lower family income and female child sex predicted caregiver distress independent of other variables. Even among families with higher income, those with the lowest income report much higher distress. Results are discussed in terms of the importance of informant choice and the use of sociodemographics as primary rather than control variables in studies of the families of chronically ill children.

A comparison of measures of adjustment, symptoms, and impairment among children with chronic medical conditions.

OBJECTIVE: To examine the concurrent and criterion validity of three instruments used to measure emotional and behavioral problems among children with chronic illness. METHOD: The Personal Adjustment and Role Skills Scale (PARS III), Child Behavior Checklist (CBCL), and Columbia Impairment Scale (CIS) were compared with each other and with structured interview-derived psychiatric diagnoses in 116 chronically ill children, aged 9 to 18 years. Three cutoff scores were used for each measure. RESULTS: The measures had good concurrent validity despite only moderate agreement with each other. Their criterion validity was only fair: levels of sensitivity were fair at low cutoffs and poor at standard and high cutoffs; specificity and positive predictive values rose from moderate at low cutoffs to high at standard and high cutoffs. The agreement between the scales and a mental disorder diagnosis was only moderate at any of the cutoff points. CONCLUSIONS: Each of these measures has significant limitations. Both the CBCL and the PARS III appear to measure similar constructs but are likely to underidentify medically ill children with comorbid psychiatric problems. Consideration of global functioning across domains, with a measure such as the CIS, might provide an alternative approach to behavioral and symptom checklists in the assessment and management of children with chronic conditions.

Psychological distress among caregivers to heart transplant recipients.

To test the hypothesis that family caregivers to heart transplant recipients may experience higher than average levels of distress during the period post-transplant and explore the correlates of distress, 83 caregivers were interviewed 3 times during the first year post-transplant and evaluated on predisposing and psychosocial characteristics. Mean distress was significantly elevated above community norms at initial assessment but subsided as the year progressed. Multiple regression analyses showed that: (a) employment status and caregivers' physical health were strong predictors of post-transplant distress while psychiatric history was not; (b) the burden of caregiving was associated with increased distress early post-transplant but not in later months; and (c) intrapersonal and social support resources early post-transplant were associated with distress both short-term and long-term. Interventions targeted at these environmental and personal factors may be important for minimizing negative effects of the transplant experience on family caregivers.

Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA.

We report here the nucleotide sequence of a clone, C2H2-34.10, isolated from a human brain cDNA library using degenerate oligodeoxyribonucleotide hybridization. C2H2-34.10 has extensive homology to the Xenopus laevis 5S DNA/RNA-binding protein, TFIIIA. The deduced amino acid (aa) sequence of the human clone gives a protein of 363 aa with identity to TFIIIA from both X. laevis (57%) and Rana pipiens (59%). This human clone contains nine C2H2-type zinc fingers like frog TFIIIA. Northern blot analysis indicates that the C2H2-34.10 RNA is expressed in human ovary, as well as human neuronal cell lines.

Rapid isolation and characterization of 118 novel C2H2-type zinc finger cDNAs expressed in human brain.

C2H2-type zinc finger genes comprise one of the largest gene families in the human genome. These proteins are involved in genetic regulation and development and are quite conserved throughout evolution. The finger domains commonly contain the small linker peptide TGEKP between some finger units. Here, we report the isolation of 133 human zinc finger cDNAs, of which 118 are novel. These clones were isolated from human brain cDNA libraries using oligonucleotide hybridization followed by expressed sequence tag (EST) analysis, sequencing from the conserved linker region using degenerate oligonucleotide primers. This directed partial sequencing approach to cDNA isolation and characterization, signature sequencing, combines the speed of EST automatic sequencing with the focus of specific cDNA family analysis. Signature sequencing minimizes the generation of less informative random EST sequences and provides a unique relative position for sequence comparison. We also show that there is an even distribution of these RNAs from this brain cDNA library, and that these cDNAs contain N-terminal domains found in other zinc finger genes. This rapid focused sequencing approach should be applicable to any family of cDNAs containing short conserved signature peptide sequences.

Depressive symptoms and adaptive style in children with cancer.

OBJECTIVE: This article explores the relationship between a repressive style of adaptation and depressive symptomatology. METHODS: Thirty-one adolescent cancer patients were compared with 83 healthy high school students. Subjects with repressive adaptation were identified as those reporting low anxiety and high defensiveness. Depressive symptomatology was measured by self-report. RESULTS: Patients with cancer reported significantly lower levels of depression, and a significantly higher proportion were identified as repressors. Repressor status accounted for significant variance in depression over that explained by illness. CONCLUSIONS: Adaptive style may be common in medically ill children and may directly and indirectly influence psychological and medical outcome.