LINC01426 aggravates the malignant progression of glioma through miR-661/Mdm2 axis.

BACKGROUND: Long intergenic non-protein coding RNA 1426 (LINC01426) is up-regulated in glioma and functions as a tumor promoter. However, the role of LINC01426 in glioma required further exploration. Therefore, this article mainly studied the role and possible mechanism of LINC01426 in glioma. METHODS: The area under the receiver operating characteristic curve was used to determine the diagnostic value of LINC01426. The effect of LINC01426 on tumor growth was analyzed by tumorigenesis assay and immunohistochemical analysis. Bioinformatics analysis, dual-luciferase assay, RNA pull-down, Pearson test, and real-time quantitative PCR (RT-qPCR) were applied to verify the relationship between target genes. The expressions and effects of LINC01426, miR-661 and MDM2 proto-oncogene (Mdm2) in glioma were examined by bioinformatics analysis combined with molecular and functional experiments (RT-qRCR, 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide, clone formation, BrdU, flow cytometry). The expressions of proliferation and apoptosis-related proteins were determined by Western blot. RESULTS: LINC01426, which was high-expressed in glioma and was related to poor prognosis, could be used as a diagnostic marker for glioma. SiLINC01426 inhibited the malignant phenotype of glioma cells in vitro and attenuated tumor growth and PCNA expression in vivo, while the effects of LINC01426 were the opposite. LINC01426 targeted and inversely correlated with miR-661, which was low-expressed in glioma. MiR-661 inhibitor evidently overturned the effect of siLINC01426 on biological functions, proliferation, and apoptosis-related proteins of glioma cells. Mdm2 bound to miR-661. Moreover, siMdm2 reversed the effects of miR-661 inhibitor on the biological characteristics and Mdm2/p53/p21 expression of glioma cells. CONCLUSION: LINC01426 aggravated the malignant progression of glioma through miR-661/Mdm2 axis.

Photochemical fabrication of 3D hierarchical Mn3O4/H-TiO2 composite films with excellent electrochemical capacitance performance.

We herein report a novel, energy-saving and environmentally benign photodeposition approach to fabricate a manganese oxide film on hydrogenated TiO2 (H-TiO2) nanotube arrays using a Mn(2+)-containing solution as a precursor. Mn(2+) ions are oxidized to Mn3O4 by the photogenerated holes during the photodeposition. The preferential growth of Mn3O4 on the nucleation sites leads to the formation of Mn3O4 nanorods on each H-TiO2 nanotube, forming a 3D hierarchical Mn3O4/H-TiO2 composite film. The as-fabricated 3D hierarchical Mn3O4/H-TiO2 composite film electrode delivers a high specific capacitance of 508 F g(-1) at a current of 0.7 A g(-1). The composite film electrode still shows a specific capacitance of 228 F g(-1) even at a high rate of 35.7 A g(-1), demonstrating its prominent rate capability. Remarkably, the composite film electrode shows no obvious capacitance decay after 10,000 charge/discharge cycles at a current density of 3.6 A g(-1), revealing its superior electrochemical cycling stability. The prominent pseudocapacitive performance of the composite film electrode can be attributed to its unique structure characteristics. The as-constructed energy-saving and environmentally benign photodeposition method can be used as a general and efficient route to prepare other composite materials with controlled morphologies and dimensions.

Photocatalytic oxidation of arsenite over TiO2: is superoxide the main oxidant in normal air-saturated aqueous solutions?

TiO(2) photocatalytic oxidation (PCO) of As(III) in the normal air-saturated aqueous solutions has been widely studied. Yet no consensus has been achieved on the mechanism whether superoxide is the main oxidant, although many approaches have been taken. (Photo)electrochemical method can minimize changes to TiO(2) surface and could therefore not alter the normal mechanism. In this Article, both this approach and As(III) oxidation kinetic measurements were performed to clarify the disputed mechanism. Under a sufficient cathodic bias potential, the dark oxidation of As(III) by superoxide could occur, but both the reaction rate and the columbic efficiency were rather low, suggesting that it is a weak oxidant. However, under UV light, both the reaction rate and the columbic efficiency were greatly enhanced even at potentials negative enough to eliminate photohole participation, indicating that more efficient oxidants than superoxide were produced. Under UV illumination and enough positive potential where superoxide was absent, the As(III) oxidation was the most highly efficient. The columbic efficiency of photoholes was much higher than that of superoxide. In the normal aerated aqueous solutions and at open circuit, although the total contribution of superoxide and its derivates to the PCO of As(III) was considerably high (up to 43%), it was not more than that of photohole (57%). In addition, the reported various approaches taken to elucidate the mechanism were discussed, and the resulting disputes can be clarified by these findings. It was demonstrated that (photo)electrochemical method could provide direct and undisputed evidence to reveal the truth mechanics issues.

Synthesis of foam-like freestanding Co3O4 nanosheets with enhanced electrochemical activities.

We report a facile and simple strategy to synthesize freestanding Co(3)O(4) nanosheets on conductive substrates. The as-prepared product shows two-dimensional hexagons in morphology with mesoporous inside architecture.

Comment on electrodeposited silicate films: importance of supporting electrolyte.

We have observed similar surface color changes of glassy carbon (GC) after anodic "electrodeposition" with the same electrolytes in the presence or the absence of silanes, indicating that sample color changes can arise from more than one thing and thus should not be used as the sole indicator of silica film formation. Nevertheless, the formation of silicate films during anodic electrodeposition is verified by X-ray photoelectron spectroscopy (XPS) measurements.