RESUMO
Objective: This study aims to help understand the mechanism behind the relationship between physical exercise and the subjective well-being among Chinese junior high school students, and it is of great significance for the intervention measures to improve the subjective well-being of junior high school students. Methods: Using stratified cluster sampling method, 1,510 junior high school students (727 males and 783 females) were measured by physical exercise rating scale, perceived social support scale, physical exercise self-efficacy scale, and subjective well-being scale. For data analysis, Pearson's correlation analysis, structural equation model test, and bias-corrected percentile Bootstrap method were carried out in turn. Results: Common method biases can be accepted in this study. (1) There is a positive correlation between physical exercise and subjective well-being, and physical exercise has a significant predictive effect on subjective well-being(ß = 0.367 t = 9.415 p < 0.01); (2) Perceived social support partially mediated the relationship between physical exercise and subjective well-being (ß = 0.08, t = 3.083, p < 0.01), and its effect ratio is 78.047%; (3) Physical exercise self-efficacy plays a partial mediating role between physical exercise and subjective well-being(ß = 0.181, t = 5.132, p < 0.01), accounting for 50.632%; (4) The chain mediating effect of perceived social support and physical exercise self-efficacy was significant (the mediating effect value was 0.028), and the effect amount was 7.629%. Conclusion: (1) Physical exercise can significantly positively predict the subjective well-being of junior high school students; (2) Physical exercise can also indirectly affect the subjective well-being of junior high school students through the mediating effect of perceived social support and physical exercise self-efficacy. The intermediary effect includes three paths, namely, the individual intermediary effect of perceived social support and physical exercise self-efficacy and the chain intermediary effect of perceived social support physical exercise self-efficacy.
RESUMO
MicroRNAs are small noncoding RNA molecules that regulate gene expression at the post-transcriptional level. Compelling evidence reveals that there is a causative link between microRNAs deregulation and lung cancer development and metastasis. The aim of present study was to explore the function of miR-140-3p in the development and metastasis of lung cancer cell. Using real-time PCR, we detected the miR-140-3p expression of lung cancer tissues and its pared non-lung cancer tissue. Then, we evaluated the role of miR-140-3p in cell proliferation, invasion and migration using MTT, colony formation assay, Transwell invasion and Transwell migration assay in lung cancer cell lines. As a result, miR-140-3p expression level was lower in lung cancer tissues compared to adjacent normal lung cancer tissue. After miR-140-3p was upregulated in A549 or H1299 cells, cell proliferation, invasion and migration was notably attenuated. Furthermore, we identified ATP6AP2, which is associated with adenosine triphosphatases (ATPases), was a directly target of miR-140-3p in lung cancer cells. In conclusion, our data suggest miR-140-3p/ATP6AP2 axis might act as a potential therapeutic biomarker for lung cancer.
