RESUMO
To understand the runoff pollution characteristics of the typical purple soil small watershed in the Three Gorges Reservoir (TGR), the rainfall-runoff duration under typical land use types of the Xinzheng watershed and the nitrogen (N) and phosphorus (P) concentrations in the outlet runoff of each catchment were dynamically monitored, and the loss rule of N and P in the rainfall runoff in the small watershed in the TGR was investigated and analyzed. The results showed that the losses of runoff N and P were 13.69 kg·(hm2·a)-1 and 1.50 kg·(hm2·a)-1, respectively. N and P in agricultural fertilizers and rainfall scouring were the main causes of runoff pollution in the Xinzheng watershed. The average concentration of total nitrogen (TN) and total phosphorus (TP) reached 10.05 mg·L-1 and 1.10 mg·L-1, far exceeding the occurrence standard of eutrophication, which should be cause for concern. The nitrate nitrogen (NN) and particulate phosphorus (PP) in the rainfall were 69.47 kg and 6.83 kg on August 15, 2010, accounting for 53.91% and 53.78% of TN and TP, respectively. The NN and AN were 6.68 kg and 5.61 kg, respectively, in the rainfall on August 26, 2010, accounting for 37.74% and 31.69% of TN and PP was 1.36 kg, accounting for 57.63% of TP, indicating that nitrogen loss was mainly through the soluble state, while phosphorus migration was dominated by particulate matter. Heavy rainfall in the Xinzheng watershed had a significant impact on the loss of nitrogen and phosphorus. This was related to the properties of purple soil, such as thin soil layer, frequent cultivation and relatively loose soil.
RESUMO
OBJECTIVE: To study the physiological mechanism of anti-stress of Coptic chinensis and provide theoretical basis for its cultivation and promoting its quality. METHODS: Different degrees of the range of time and intensity of UV-B radiation were set in the experiment. Used the technique of polyacrylamide gelatin vertical board electrophoresis (PAGE) to analyse the isozyme and related stress index. RESULTS: The isoenzymic bands of SOD1 (Rf = 0.125), SOD2 (Rf = 0.312), CAT1 (Rf = 0.428), POD3 (Rf = 0.290), POD4 (Rf = 0.636) were induced by UV-B radiation after 3 hours, with the increase of the time of UV-B radiation, those isoenzymic bands was going to vanish or became unclear. Moreover, isoenzymic bands of CAT1 (Rf = 0.428), POD3 (Rf = 0.290) disappeared in advance under heavy intensity of UV-B radiation. Furthermore, the contents of MDA, soluble sugar, proline were higher dramatically than those of control group under UV-B radiation. However, excluding the increases of proline in UL group, the content of MDA, soluble sugar, proline of other groups commenced to decrease slowly and isoenzymic bands of soluble protein increase after 7 hours of UV-B radiation. CONCLUSION: The increase of the expression of antioxidase isozyme, accumulation of soluble sugar, soluble protein and other antioxidase matter is induced by the short-time UV-B radiation, which can protect Coptis chinensis from being harmed by UV-B radiation. However, regulation system of Coptis chinensis are broken, metabolism is disordered, the bands of antioxidase isozyme vanish or weaken, the bands of soluble protein are increased and widened, these phenomenon is caused by 7 hours of UV-B radiation.
