[Effects of two implant systems on peri-implant soft tissue].

PURPOSE: To investigate the effect of different implant systems on peri-implant soft tissue. METHODS: Forty patients requiring single implant therapy in posterior teeth at Dental and Ophthalmic Clinic of Putuo District from December 2020 to February 2021 were selected. Ten patients were implanted with bone level implants and 30 patients with soft tissue level implants. Periodontal exploration was performed at the buccal side, lingual side, mesial and distal axial angle of the implants, and the gingival crevicular fluid of these locations were taken to detect the enzyme level. The probing depth, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) level of the two groups were compared at the day, 3 months, 6 months and 12 months after crown restoration. Statistical analysis was completed by SPSS 17.0 software package. RESULTS: The probing depth and ALP level of soft tissue level implants were significantly lower than those of bone level implants at the day, 3 months, 6 months and 12 months after crown restoration(P＜0.05). At the day when crown restoration was accomplished, AST of soft tissue implant was significantly lower than that of bone level implant(P＜0.05). AST in gingival crevicular fluid of bone level implants decreased rapidly in the following three time periods, and close to that of the soft tissue level implants(P＞0.05) at last. CONCLUSIONS: Both bone level implants and soft tissue level implants have good clinical effects, but peri-implant soft tissues of the soft tissue level implants show better stability.

[Comparative study on the accuracy of implant-borne single restoration by two production processes with Ti-base].

PURPOSE: To study on the accuracy of implant-borne single restoration by two production processes with Ti-base to provide experimental data for proper processes of single implant-borne restoration. METHODS: Thirty patients were selected with single posterior teeth missing from the Department of Oral Implantology of Shanghai Putuo District Eye Disease and Dental Disease Prevention and Treatment Institute. The patients were taken 2 traditional impressions clinically for two plaster model equipped with implant analogue. These models were then divided into 2 groups according to different production processes. The experimental group was scanned with the scan body installed in the model implant analogue, while the control group was scanned directly on the Ti-base abutment installed in the model implant analogue. The implant-borne single restorations of the two groups were cut along the buccal-lingual side and the distance between the measuring point to the Ti-Base abutment was observed by electron microscopy. In addition, the breaking limit of zirconia crown was observed, universal test machine was used to load direct force to the crown. SPSS 22.0 software package was used for data analysis. RESULTS: The gap between the implant-borne single restoration to the Ti-base abutment of the experimental group was significantly smaller than that of the control group. The difference between the two groups was statistically significant (P<0.05). However, by testing the breaking limit of zirconia crown, there was no significant difference(P>0.05). CONCLUSIONS: Using scan body to transfer the implant position and Ti-base abutment data information to the digital dental design software is more accurate and reliable than directly scanning the Ti-base on the model analogue. Using scan body is recommended for processing and manufacture of implant-borne singe restoration.

[Clinical effectiveness evaluation of gingival recession by using perioscope as an adjunct to non-surgical periodontal therapy].

PURPOSE: To investigate the effects of ultrasonic scaling and root planning(SRP) assisted by perioscope on gingival recession of maxillary lateral incisor. METHODS: Thirty-six outpatients with moderate to advanced chronic periodontitis from the Department of Periodontology at Dental and Ophthalmic Clinic of Putuo District from June 2020 to December 2020 were collected as research objects. Periodontal treatment was carried out according to a single-blind split-mouth self-control design randomly with(experimental group, namely perioscope group) or without(control group, namely SRP group) periodontal endoscope．The labial periodontal probing depth (PD), labial attachment loss (AL) and gingival recession(GR) in the maxillary lateral incisors were recorded at baseline, 3 and 6 months, and compared among groups by SPSS 22.0 software package. RESULTS: There was no significant difference between perioscope group and SRP group at baseline. ΔGR (the recession extent of gum within two observation time) in perioscope group was significantly smaller than that in SRP group at 3 months（P<0.05）. There was no significant difference in other periodontal indicators at 3 and 6 months between the 2 groups after treatment, but it can be found that the degree of PD reduction and AL improvement in perioscope group was more than those in SRP group, this trend was most obvious at 3 months. PD and AL were significantly different between baseline and 3 months or 6 months in the two groups. There were significant differences in ΔGR at 3 months and 6 months between the two groups. CONCLUSIONS: Compared with routine SRP, the extent of root surface debridement with perioscope-assisted SRP is thorough and less invasive, and the reduction of gingival recession of labial surface of maxillary lateral incisor at 3 months is significantly less; thus, the aesthetic effect is prominent.

The octanol/water distribution coefficients of ardipusilloside-I and its metabolites, and their permeation characteristics across Caco-2 cell monolayer.

BACKGROUND: Ardipusilloside-I (ADS-I) is a triterpenoid saponin extracted from Chinese medicinal herb Ardisiapusill A. DC. Previous studies have demonstrated the potent anti-tumor activities of ADS-I both in vitro and in vivo, and its main metabolites (M1 and M2) from human intestinal bacteria. However, the physicochemical properties and intestinal permeation rate of ADS-I and its metabolites are not understood. In this study, the octanol/water distribution coefficients (logP) of ADS-I and metabolites were investigated using standard shake flask technique, and their permeability properties was investigated across Caco-2 cells monolayer. RESULTS: The logP of ADS-I, M1 and M2 was -0.01, 0.95 ± 0.04, 1.57 ± 0.11, respectively. The Papp values of ADS-I, M1 and M2 (in 10 µmol/L) across Caco-2 cell monolayers from the apical (AP) to basolateral (BL) direction were 1.88 ± 0.21 × 10(-6) cm·s(-1), 4.30 ± 0.43 × 10(-6) cm·s(-1), 4.74 ± 0.47 × 10(-6) cm·s(-1), respectively. CONCLUSION: Our data indicated that ADS-I has the poorer intestinal absorption than its metabolites (M1 and M2) in these experimental systems, suggesting that the metabolites of ADS-I may be the predominant products absorbed by the intestine when ADS-I is administered orally.

Ardipusilloside-I Metabolites from Human Intestinal Bacteria and Their Antitumor Activity.

Ardipusilloside-I (ADS-I) is a triterpenoid saponin extracted from Ardisia pusilla DC, and has been demonstrated to have potent antitumor activity. However, ADS-I metabolism in humans has not been investigated. In this study, we studied the biotransformation of ADS-I in human intestinal bacteria, and examined the in vitro antitumor activity of the major metabolites. Ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to detect ADS-I biotransformation products, and their chemical structures were identified by high performance liquid chromatography-nuclear magnetic resonance (HPLC-NMR). The antitumor activity of the major metabolites was determined by the MTT assay. Here, we show that main reaction seen in the metabolism of ADS-I in human intestinal bacteria was deglycosylation, which produced a total of four metabolites. The structures of the two major metabolites M1 and M2 were confirmed by using NMR. MTT assay showed that ADS-I metabolites M1 and M2 have the same levels of inhibitory activities as ADS-I in cultured SMMC-7721 cells and MCF-7 cells. In conclusion, this study demonstrates deglycosylation as a primary pathway of ADS-I metabolism in human intestinal bacteria, and suggests that the pharmacological activity of ADS-I may be mediated, at least in part, by its metabolites.