Effect of in situ vermicomposting combined with biochar application on soil properties and crop yields in the tomato monoculture system.

Vermicompost and biochar have been widely used to improve soil conditions. However, little information is available regarding the efficiency and effectiveness of in situ vermicomposting with biochar (IVB) in monoculture soils. In this study, we estimated the effects of IVB on soil physiochemical and microbial properties, crop yields, and fruit quality under the tomato monoculture system. The soil treatments considered were (i) untreated monoculture soil (MS, control), (ii) MS plus 1.5 t/ha biochar applied to soil surface (MS+1.5BCS), (iii) MS plus 3 t/ha biochar applied to soil surface (MS+3BCS), (iv) MS mixed with 1.5 t/ha biochar (MS+1.5BCM), (v) MS mixed with 3 t/ha biochar (MS+3BCM), (vi) in situ vermicomposting (VC), (vii) VC plus 1.5 t/ha biochar applied to VC surface (VC+1.5BCS), (viii) VC plus 3 t/ha biochar applied to VC surface (VC+3BCS), (ix) VC mixed with 1.5 t/ha biochar (VC+1.5BCM), and (x) VC mixed with 3 t/ha biochar (VC+3BCM). In general, soil pH varied from 7.68 to 7.96 under VC-related treatments. The microbial diversity was much higher in bacterial communities (OTU: 2284-3194, Shannon index: 8.81-9.91) than in fungal communities (OTU: 392-782, Shannon index: 4.63-5.71) in VC-related treatments. Specifically, Proteobacteria was the dominant bacterial phylum, followed by Bacteroidota, Chloroflexi, Patescibacteria, Acidobacteriota, Firmicutes, and Myxococcota. It is worth noting that IVB-related treatments could increase the relative abundance of Acidobacteria and reduced the relative abundance of Bacteroidetes. In addition, the VC+1.5BCM treatment exhibited the greatest yield (9377.6 kg/667m2) and simultaneously showed higher fruit quality (vitamin C, 28.94 mg/100g; soluble sugar, 20.15%) as compared to other treatments. Our results suggested that in situ vermicomposting with biochar can improve soil properties and enhance both crop yields and fruit quality under the tomato monoculture system.

Compost Addition Attenuates the Negative Impacts of High Soil Mineral Nitrogen Levels on Rhizosphere Microbial Characteristics and Enhances Cucumber Growth in Monoculture Systems.

Due to the increase in the human population, it is necessary to seek efficient methods of increasing crop productivity and, simultaneously, sustaining the soil. One way is to grow high demand crops continuously without rotating with other crops. This practice is often accompanied by increased rates of fertilizer application that can affect efficient nitrogen (N) cycling in the plant rhizosphere soil which, in turn, affects both plant growth and environmental pollution. In the present study, twelve various cucumber soils were selected from monoculture systems presenting different cropping years and divided into two groups including soils with relatively high mineral N (HMN) content (N > 100 mg kg−1 soil) and those with a lower mineral N (LMN) content (N < 100 mg kg−1 soil). All soils were amended with the addition of compost alone or in combination with bacterial inoculation to evaluate their effects on plant growth, microbial numbers, N mineralization, and N cycling genes. In general, the HMN soils increased (p < 0.05) net N mineralization (NNM) but did not statistically (p > 0.05) affect plant biomass compared to the LMN soils; however, compost addition increased both NNM and plant biomass in the HMN soils. In addition, the HMN soils had higher fungal pathogen numbers (FPNs) but lower total microbial biomass (TMB) and bacterial numbers (BNs) compared to the LMN soils; however, compost addition decreased FPNs but increased TMB and BNs in the HMN soils (all p < 0.05). Plant biomass was positively related to TMB, BN and NNM but was negatively related to FPN (all p < 0.05). In summary, compost addition reduced the high mineral N levels' adverse effects on the rhizosphere soil and plant growth.

Use of Carbon Nanoparticles to Improve Soil Fertility, Crop Growth and Nutrient Uptake by Corn (Zea mays L.).

The use of carbon nanoparticles (CNPs) as a fertilizer synergist to enhance crop growth has attracted increasing interest. However, current understanding about plant growth and soil response to CNPs is limited. In the present study, we investigated the effects of CNPs at different application rates on soil properties, the plant growth and nutrient use efficiency (NUE) of corn (Zea mays L.) in two agricultural soils (Spodosol and Alfisol). The results showed that CNPs affected corn growth in a dose-dependent manner, augmenting and retarding growth at low and at high concentrations, respectively. The amendment at the optimal rate of 200 mg CNPs kg-1 significantly enhanced corn growth as indicated by improved plant height, biomass yield, nutrient uptake and nutrient use efficiency, which could be explained by the higher availability of phosphorus and nitrogen in the amended soils. The application of CNPs largely stimulated soil urease activity irrespectively of soil types. However, the responses of dehydrogenase and phosphatase to CNPs were dose dependent; their activity significantly increased with the increasing application rates of CNPs up to 200 mg kg-1 but declined at higher rates (>400 mg kg-1). These findings have important implications in the field application of CNPs for enhancing nutrient use efficiency and crop production in tropical/subtropical regions.

Vermicomposting of livestock manure as affected by carbon-rich additives (straw, biochar and nanocarbon): A comprehensive evaluation of earthworm performance, microbial activities, metabolic functions and vermicompost quality.

Vermicomposting is an eco-friendly method for treating organic wastes. This study investigated the effects of the addition of straw (S), biochar (B), nanocarbon (N), S + B and S + N to cow dung (CD) on earthworm (Eisenia fetida) performance, microbial properties and vermicompost quality. In general, the earthworm growth rate and cocoon production were enhanced by straw addition, but were inhibited by biochar or nanocarbon addition. However, biochar and nanocarbon increased microbial communities associated with organic matter decomposition, and improved metabolic functions, enzyme activities and vermicompost properties. Moreover, addition of straw in combination with nanocarbon resulted in the highest vermicompost quality index (VQI), and significantly increased the biomass of three different test crops (radish, lettuce and pakchoi). These results indicated that biochar and nanocarbon mainly improved microbial activities, while straw primarily enhanced earthworm performance during vermicomposting. In addition, straw combined with nanocarbon can be used to enhance the agronomic performance of vermicompost.

Transcriptional changes during tomato ripening and influence of brackish water irrigation on fruit transcriptome and sugar content.

Efficient management and utilization of brackish water irrigation help to minimize yield losses and promote fruit quality and sugar content in tomato fruit. However, the functional genes involved in sugar metabolic pathways and potential molecular pathways responsive to brackish water irrigation remain unknown. To this end, physiological responses and comparative transcriptional profiling was used to analyze the tomato fruit during the white-ripe period (CK1) and mature period (CK2) in plants grown under four water management strategies (rotating irrigation with brackish and fresh water during fruit development, T1; fresh water irrigation, T2; mixed brackish and fresh water irrigation, T3; mixed water and fresh water irrigation in sequence, T4). Comparative analysis revealed that during fruit development (CK2 cv CK1) differentially expressed genes (DEGs) involved in photosynthetic pathways and sucrose-starch metabolism were downregulated. However, two DEGs encoding putative beta-fructofuranosidases were significantly upregulated at the mature stage, which promoted the accumulation of glucose and fructose in CK2. Comparing four types of management strategies, rotating irrigation with brackish water and fresh water (T1) led to reprograming of global gene expression. Moreover, the upregulated DEGs in T1 were significantly enriched for signaling, hormone metabolism, and stress tolerance, suggesting the coordination of both stresses signaling as well as the plant hormone. These results provide a valuable reference for rational use of brackish water in the production of high-quality tomato in arid and semi-arid regions.

Biochar-enhanced composts reduce the potential leaching of nutrients and heavy metals and suppress plant-parasitic nematodes in excessively fertilized cucumber soils.

Excessive fertilization is a common agricultural practice that has largely reduced soil nutrient retention capacity and led to nutrient leaching in China. To reduce nutrient leaching, in this study, we evaluated the application of biochar, compost, and biochar-compost on soil properties, leaching water quality, and cucumber plant growth in soils with different nutrient levels. In general, the concentrations of nutrients and heavy metals in leaching water were higher under high-nutrient conditions than under low-nutrient conditions. Both biochar and compost efficiently enhanced soil cation exchange capacity (CEC), water holding capacity (WHC), and microbial biomass carbon (MBC), nitrogen (MBN), and phosphorus (MBP), reduced the potential leaching of nutrients and heavy metals, and improved plant growth. The efficiency of biochar and compost in soil CEC, WHC, MBC, MBN, and MBP and plant growth was enhanced when applied jointly. In addition, biochar and biochar-enhanced compost efficiently suppressed plant-parasitic nematode infestation in a soil with high levels of both N and P. Our results suggest that biochar-enhanced compost can reduce the potential environmental risks in excessively fertilized vegetable soils.