New protocols to improve the deposition and hatching of Sepia officinalis' eggs.

The objective of this study was the development of hatching protocols in controlled conditions to obtain juveniles, in order to restock and increase the resource of Sepia officinalis. The study was divided into the following phases: development and application of artificial surfaces at specific sites of the Molise coast in Italy; induction of eggs hatching and juveniles maintenance under controlled condition; juveniles introduction into specific sites and assessment their increment; experimental data elaboration. The obtained results concerned both the effectiveness of the artificial surfaces tasted during the study and the importance of the recovery of the eggs laid on artificial surfaces (artefacts and fishing gear) for preservation and the management of the Sepia officinalis resource. The induction tests conducted on eggs hatching under controlled conditions confirmed what described in the extant literature. Water salinity was detected as the only limiting factor, with values ≤ 20% related to the absence of hatching. The described practices for harvesting and induction of hatching for the production of juvenile cuttlefish may be endorsed by the operators at relatively low cost and throughout the year, with obvious economic benefits.

Study of the efficacy of a closed cycle depuration system on bivalve molluscs.

The aim of this study was to develop and apply protocols for the microbiological depuration of bivalve molluscs (Chamelea gallina and Mitylus galloprovincialis). The study was divided into the following phases: preparation of two closed-circuit tanks fitted with mechanical, chemical and biological filtration systems for the depuration of bivalve molluscs, mollusc contamination and depuration, analyses of molluscs and depuration waters (biometric and microbiological tests) and statistical processing of the experimental data. The Escherichia coli challenge revealed the high efficacy of the depuration system and thus the possibility of preparing the molluscs for sale in a relatively short period of time. Depuration tests against Salmonella Typhimurium were effective only after 72 h to 84 h. Results for Vibrio parahaemolyticus revealed only a small reduction in the bacterial load during the entire observation period. However, depuration times were shorter in trials with mussels: V. parahaemolyticus was no longer detected after 36 h to 48 h.