Clonación del gen caf1 de yersinia pestis en el centro nacional de productos biológicos del Instituto Nacional de Salud / Cloning of the caf1 gene from yersinia pestis at the National Center for Biological Products of the National Institute of Health

El objetivo del presente trabajo fue amplificar y clonar la secuencia codificante del gen caf1 de Yersinia pestis en el plásmido pET32a (+). Para esta investigación, se empleó una cepa nativa Y.

Comparative study of bacterial microfiltration in the implant-abutment interface, with straight and conical internal connections, in vitro.

OBJECTIVE: to determine the presence of marginal bacterial microfiltration in the IAI in different implant/abutment systems, in vitro. MATERIAL AND METHODS: Fifty-six implants from seven different brand names, 4 with cone and 3 with straight connections were used, implant and abutment were connected using the Ncm tightening as indicated by each of the manufacturers and then were sealed. The samples were subjected occlusal load and thermal cycling, a first sample of each group was observed by micro CT and in a second sample (both samples randomly selected) length of connection was measured, while the rest of the samples were mounted on devices according to the bacterial microfiltration model with Porphyromonas gingivalis. RESULTS: Two of the conical connection system groups did not present bacterial microfiltration, one of the three straight connection groups only microfiltered in one sample, while the other two conical as well as the two straight connection samples showed different and important levels of bacterial microfiltration, all groups presented a direct relationship between the implant-abutment adjustment determined by micro-CT and bacterial microfiltration levels, not related to the connection length. CONCLUSION: Only two conical connection systems presented no bacterial microfiltration.

Characterization of influenza A(H1N1)pdm09 isolates of Peru using HRM, a post PCR molecular biology method.

Influenza caused by A(H1N1)pdm09 is a public health issue with severe conditions in vulnerable populations leading to death. Therefore, it is of interest to characterize and monitor influenza A(H1N1)pdm09 genotypes using High Resolution Melting (HRM), a post PCR molecular biology method. We used HRM analysis (using RotorGene Q thermocycler) to characterize A(H1N1)pdm09 genotypes from several places of Peru. RNA was purified from nasal and pharyngeal swab samples referred to LRNVR-INS, synthesized cDNA, and then the hemagglutinin gene and matrix fragment were amplified. Thus, 287 samples positive for influenza A(H1N1)pdm09 were identified across Peru where places like Lima, Piura, and Arequipa documented highest number of cases. The HRM data was analyzed and results showed different profiles which were further grouped into four genotypes for the HA (A, B, C, D) and 3 for the M (a, b, c) genes. We also report ten genotypes (I-X) of virus using combined HA (hemagglutinin) and M gene profiles representing a national geography. The prevalent genotypes are I and II with a frequency of 35.89% (103) and 29.27% (84), respectively linking with severe acute respiratory infection.

Virus interference on local scale viticulture: the case of Moll variety from Majorca (Spain)

In Europe, many autochthonous grapevines (Vitis vinifera L.) are only cultivated at local scale, but play a very important economic role due to their strict relation with terroir and wine tipicity. In this study, it was pursued to evaluate the influence of several factors on performance of Moll, the main autochthonous white variety of Majorca, by means of the database coming from a clonal preselection. The effects of multiple virus infections, vintage and vineyard components were studied by univariate linear models and principal component analysis, starting from measurement of production and must quality parameters of several vines located in 14 vineyards belonging to two appellations during four consecutive years (2001-2004). Absence of multiple virus infections, double cordon system, high clay content and Useful Water Reserve in soils have enhanced vine production without inducing considerable alterations in sugar accumulation in berries and acidity. Moll variety presented great viticultural and oenological potentials. Fertile and deep soils should be preferred in order to maximize production. Also, the use of certified propagation material is strongly recommended. However, further investigations are required to optimize must quality by opportune managements. This study does not provide only essential information to improve Moll cultivation in Majorca, but it also represents a useful example to analyze grapevine varieties that are endemically infected by viruses. In fact, in such situations, it may be supposed an insidious interference by viruses on terroir and wine tipicity.(AU)

Virus interference on local scale viticulture: the case of Moll variety from Majorca (Spain)

In Europe, many autochthonous grapevines (Vitis vinifera L.) are only cultivated at local scale, but play a very important economic role due to their strict relation with terroir and wine tipicity. In this study, it was pursued to evaluate the influence of several factors on performance of Moll, the main autochthonous white variety of Majorca, by means of the database coming from a clonal preselection. The effects of multiple virus infections, vintage and vineyard components were studied by univariate linear models and principal component analysis, starting from measurement of production and must quality parameters of several vines located in 14 vineyards belonging to two appellations during four consecutive years (2001-2004). Absence of multiple virus infections, double cordon system, high clay content and Useful Water Reserve in soils have enhanced vine production without inducing considerable alterations in sugar accumulation in berries and acidity. Moll variety presented great viticultural and oenological potentials. Fertile and deep soils should be preferred in order to maximize production. Also, the use of certified propagation material is strongly recommended. However, further investigations are required to optimize must quality by opportune managements. This study does not provide only essential information to improve Moll cultivation in Majorca, but it also represents a useful example to analyze grapevine varieties that are endemically infected by viruses. In fact, in such situations, it may be supposed an insidious interference by viruses on terroir and wine tipicity.