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Biochem J ; 167(2): 501-4, 1977 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-202255

RESUMO

Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay appears to be specific. However, interference by hitherto unrecognized enzymes of abnormal sera must be ruled out.


Assuntos
Peptidil Dipeptidase A/análise , Hipuratos , Oligopeptídeos , Trítio
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