Ferroelectret-based Hydrophone Employed in Oil Identification-A Machine Learning Approach.

This work focuses on acoustic analysis as a way of discriminating mineral oil, providing a robust technique, immune to electromagnetic noise, and in some cases, depending on the applied sensor, a low-cost technique. Thus, we propose a new method for the diagnosis of the quality of mineral oil used in electrical transformers, integrating a ferroelectric-based hydrophone and an acoustic transducer. Our classification solution is based on a supervised machine learning technique applied to the signals generated by an in-home built hydrophone. A total of three statistical datasets entries were collected during the acoustic experiments on four types of oils. The first, the second, and third datasets contain 180, 240, and 420 entries, respectively. Eighty-four features were considered from each dataset to apply to two classification approaches. The first classification approach is able to distinguish the oils from the four possible classes with a classification error less than 2%, while the second approach is able to successfully classify the oils without errors (e.g., with a score of 100%).

Altered phenotype and function of dendritic cells in individuals with chronic periodontitis.

OBJECTIVE: To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP). DESIGN: m-MDDCs derived from peripheral blood monocytes, cultured for 7 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF), were stimulated with lysates of Streptococcus sanguinis, Prevotella intermedia, Porphyromonas gingivalis, or Treponema denticola on day 4, and were then phenotyped. IL-10, IL-12 and IFN-gamma concentration in the supernatant of cultures were measured. RESULTS: Expression of HLA-DR was lower in bacterial-unstimulated mature m-MDDC from CP compared to HP (p=0.04), while expression of CD1a and CD123 were higher in CP. The expression pattern of HLA-DR, CD11c, CD123, and CD1a did not change on bacterial stimulation, regardless of the bacteria. Stimulation with P. intermedia upregulated CD80 and CD86 in CP cells (p≤0.05). Production of IL-12p70 by bacterial-unstimulated m-MDDCs was 5.8-fold greater in CP compared to HP. Bacterial stimulation further increased IL-12p70 production while decreasing IL-10. Significantly more IFN-gamma was produced in co-cultures of CP m-MDDCs than with HP m-MDDCs when cells were stimulated with P. intermedia (p=0.009). CONCLUSIONS: Bacterial-unstimulated m-MDDC from CP exhibited a more immature phenotype but a cytokine profile biased towards proinflammatory response; this pattern was maintained/exacerbated after bacterial stimulation. P. intermedia upregulated co-stimulatory molecules and IFN-gamma expression in CP m-MDDC. These events might contribute to periodontitis pathogenesis.

FBG in PVC foils for monitoring the knee joint movement during the rehabilitation process.

This paper presents a sensing electronic-free wearable solution for monitoring the body kinematics. The measuring of the knee movements, flexion and extension, with the corresponding joint acting as the rotation axis is shown as working principle. The proposed sensing system is based on a single optical Fiber-Bragg Grating (FBG) with a resonance wavelength of 1547.76 nm. The optical fiber with the FBG is placed inside a new polymeric foil composed by three flexible layers which facilitates its placement in the anatomic parts under investigation while maintaining full sensing capabilities. The way the device is placed in the specific body part to be measured enables the clear detection of the movements in respect to the joint. The proposed solution was tested using a prototype that was built to evaluate the device under different condition tests and also to assess the system's consistency. The designed and fabricated system demonstrates clear advantages in medical fields like physical therapy applications as optical fiber is not affected by electromagnetic interference nor does the system needs complex and expensive electronic systems and mechanical parts. Another advantage is the possibility to measure, record and evaluate specific mechanical parameters of the limbs' motion. Patients with bone, muscular and joint related health conditions, as well as athletes, are within the most important end-user applications.

TNF-alpha activates human monocytes for Paracoccidioides brasiliensis killing by an H2O2-dependent mechanism.

Human monocytes activated by recombinant tumor necrosis factor alpha (TNF-alpha) exhibited significant fungicidal activity on the yeast cells of a highly virulent strain of Paracoccidioides brasiliensis. This process was significantly inhibited in the presence of catalase (CAT - a scavenger of H2O2), but not in the presence of superoxide-dismutase (SOD - a scavenger of superoxide anion) or NG-monomethyl-L-arginine (NG-MMLA - a nitric oxide inhibitor). Furthermore, there was a direct association between the intracellular killing of the fungus and the production of H2O2 by activated cells. These results strongly suggest a role for H2O2 in the killing of highly virulent strains of P. brasiliensis by TNF-alpha-activated human monocytes.