RESUMO
Rubella virus was isolated from the brain of a congenitally-infected, 12-year-old child in whom progressive mental and motor deterioration became evident at age 8 and 11 years respectively. The virus was initially isolated in a co-culture of CV-I cells with the trypsinized brain tissue; subsequently the culture of the brain tissue also showed evidence of rubella virus infection recognized by indirect fluorescent antibody technique (IFA) using anti-rubella virus antibody prepared in rabbits as intermediate serum. Both isolates interfered with infection of BSC-I cell lines by echovirus type II. The interfering virus was identified as rubella virus by IFA with the specific antiserum, and it is designated as the NTr strain of rubella virus. The complement fixing antibody titre to rubella virus in serum was I:256. The spinal fluid was anticomplementary. Rubella virus haemagglutinating antibody titre (HI) in serum was I:8196 and in the spinal fluid I:128. The HI antibody was of the IgG class. The corresponding HI titres to rubeola virus in serum and spinal fluid were I:8 and less than I:2 respectively.
Assuntos
Encéfalo/microbiologia , Encefalite/microbiologia , Vírus da Rubéola/isolamento & purificação , Anticorpos Antivirais/análise , Linhagem Celular , Líquido Cefalorraquidiano/imunologia , Criança , Imunofluorescência , Humanos , Vírus da Rubéola/imunologia , VírionRESUMO
Late-onset chronic progressive panencephalitis developed in a 12-year-old boy with congenital rubella syndrome from whose brain rubella virus was isolated. Progressive dementia began at eight, and ataxia, choreiform movements, myoclonic seizures, and fine perimacular pigmentation appeared at 11 years of age. The cerebrospinal fluid was minimally pleocytotic and had a total protein of 156 mg per deciliter, of which 52 per cent was gamma globulin. Electroencephalography demonstrated generalized medium and occasional high-voltage slowing without burst suppression. The antibody titer to rubella virus (hemagglutination inhibition) was 1:8192 in serum and 1:256 in cerebrospinal fluid. Antibody titer to measles virus (complement fixation) was less than 1:8 in serum. Microscopical study of biopsied brain tissue at the age of 11 disclosed panencephalitis similar to subacute sclerosing panencephalitis, but with perivascular deposits and without inclusion bodies. Rubella virus was isolated from the brain by cocultivation with CV-1 cells.
Assuntos
Encefalite/diagnóstico , Rubéola (Sarampo Alemão)/congênito , Panencefalite Esclerosante Subaguda/diagnóstico , Anticorpos Antivirais/análise , Encéfalo/microbiologia , Encéfalo/patologia , Córtex Cerebral/patologia , Proteínas do Líquido Cefalorraquidiano/análise , Criança , Doença Crônica , Diagnóstico Diferencial , Eletroencefalografia , Encefalite/microbiologia , Encefalite/patologia , Encefalite/fisiopatologia , Humanos , Corpos de Inclusão Viral , Masculino , Vírus do Sarampo/imunologia , Rubéola (Sarampo Alemão)/complicações , Rubéola (Sarampo Alemão)/microbiologia , Vírus da Rubéola/imunologia , Vírus da Rubéola/isolamento & purificação , Síndrome , gama-Globulinas/líquido cefalorraquidianoAssuntos
Cálcio/farmacologia , Junção Neuromuscular/efeitos dos fármacos , Fenitoína/farmacologia , Acetilcolina/farmacologia , Potenciais de Ação/efeitos dos fármacos , Animais , Permeabilidade da Membrana Celular/efeitos dos fármacos , Eletrodos , Potenciais Evocados/efeitos dos fármacos , Técnicas In Vitro , Membranas/efeitos dos fármacos , Fenobarbital/farmacologia , Rana pipiens , Fatores de TempoRESUMO
NERVES STAINED WITH ONE OF THE FOLLOWING FLUOROCHROMES WERE FOUND TO RESPOND TO ELECTRIC STIMULATION WITH TRANSIENT CHANGES IN THE FLUORESCENT LIGHT: acridine orange, acridine yellow, 8-anilinonaphthalene-1-sulfonate, auramine O, 1-dimethylaminonaphthalene-5-sulfonyl chloride, fluorescein isothiocyanate, LSD, pyronin B, pyronin Y, rhodamine B, rhodamine G, rose bengal, and 2-toluidinylnaphthalene sulfonate. The properties of covalently bonded fluorochromes in the nerve and the behavior of fluorochromes introduced into the axon interior were studied. By measuring fluorescence polarization, direct evidence was obtained showing a decrease in intramembrane viscosity during the action potential.
Assuntos
Corantes Fluorescentes , Neurônios/fisiologia , Acridinas , Potenciais de Ação , Compostos de Anilina , Animais , Axônios/fisiologia , Benzoatos , Crustáceos , Estimulação Elétrica , Fluoresceínas , Fluorescência , Dietilamida do Ácido Lisérgico , Moluscos , Naftalenos , Fibras Nervosas Mielinizadas/fisiologia , Compostos de Amônio Quaternário , Rosa Bengala , Ácidos Sulfônicos , XantenosAssuntos
Luz , Contração Muscular , Músculos/fisiologia , Potenciais de Ação , Animais , Anuros , Computadores , Soluções Hipertônicas , Métodos , SacaroseRESUMO
Electrical stimulation of frog striated muscle was found to prduce trasient changes in turbidity. birefringence, and fluorescence (of fibers stained with pyronine B). The initial phase of these optical changes Was coinicident with the action potential. These findings suggest that there is a macromolecular conformational change in muscle membrane during excitation.
Assuntos
Potenciais de Ação , Miofibrilas , Óptica e Fotônica , Animais , Anuros , Birrefringência , Estimulação Elétrica , Potenciais Evocados , Fluorescência , Técnicas In Vitro , Coloração e RotulagemRESUMO
Nerves from spider crabs anid squid fluoresce when staiined with Acridine Orange. The intenisity of fluorescence increases during nerse conduction. Prolongation of the electric response in the squid axon is associated with a fluorescence change of similar duration. These findings suggest that the physicochemical properties of the macromolecules around the dye molecules in the nerve membrane drastically change during the process of nerve conduction.
