RESUMO
In this report, we describe the partial molecular characterisation of Plasmodium falciparum isolates obtained from two individuals who were involved in a probable case of accidental malaria transmission after admission to a hospital in the metropolitan area of São Paulo, Brazil. Molecular analysis of polymorphic stretches of the merozoite surface protein 1 and 2 genes using PCR-typing and nucleotide sequencing revealed that the two isolates were identical and that the identified msp-1 gene was different from all others published to date. Additional anamnestic data supported our findings and made all other possible routes of infection unlikely. The methodology used here is simple to perform and needs as little as one Giemsa-stained blood smear as starting material.
Assuntos
Corantes Azur , Infecção Hospitalar/transmissão , Malária Falciparum/transmissão , Plasmodium falciparum/isolamento & purificação , Adulto , Animais , Antígenos de Protozoários/genética , Antígenos de Protozoários/isolamento & purificação , Sequência de Bases , Pré-Escolar , Infecção Hospitalar/diagnóstico , Feminino , Testes Hematológicos , Humanos , Malária Falciparum/diagnóstico , Masculino , Proteína 1 de Superfície de Merozoito/genética , Proteína 1 de Superfície de Merozoito/isolamento & purificação , Dados de Sequência Molecular , Plasmodium falciparum/genética , Reação em Cadeia da Polimerase/métodos , Proteínas de Protozoários/genética , Proteínas de Protozoários/isolamento & purificaçãoRESUMO
In this report, we describe the partial molecular characterization of Plasmodium falciparum isolates obtained from two individuals who were involved in a probable case of occidental malaria transmission after admission to a hospital in the metropolitan area of Sao Paulo, Brazil. Molecular analysis of polyphormic stretches of the merozoite surface protein 1 and 2 genes using PCR-typing and nucleotide sequencing revealed that the two isolates were identical and that the identified msp-1 gene was different from all others published to date. Additional anamnestic data supported our findings and made all other possible routes of infection unlikely. The methodology used here is simple to perform and needs as little as one Giemsa-stained blood smear as starting material.
