Encephalomyocarditis virus 2A protein is required for viral pathogenesis and inhibition of apoptosis.

The encephalomyocarditis virus (EMCV), a Picornaviridae virus, has a wide host spectrum and can cause various diseases. EMCV virulence factors, however, are as yet ill defined. Here, we demonstrate that the EMCV 2A protein is essential for the pathogenesis of EMCV. Infection of mice with the B279/95 strain of EMCV resulted in acute fatal disease, while the clone C9, derived by serial in vitro passage of the B279/95 strain, was avirulent. C9 harbored a large deletion in the gene encoding the 2A protein. This deletion was incorporated into the cDNA of a pathogenic EMCV1.26 strain. The new virus, EMCV1.26Δ2A, was capable of replicating in vitro, albeit more slowly than EMCV1.26. Only mice inoculated with EMCV1.26 triggered death within a few days. Mice infected with EMCV1.26Δ2A did not exhibit clinical signs, and histopathological analyses showed no damage in the central nervous system, unlike EMCV1.26-infected mice. In vitro, EMCV1.26Δ2A presented a defect in viral particle release correlating with prolonged cell viability. Unlike EMCV1.26, which induced cytopathic cell death, EMCV1.26Δ2A induced apoptosis via caspase 3 activation. This strongly suggests that the 2A protein is required for inhibition of apoptosis during EMCV infection. All together, our data indicate that the EMCV 2A protein is important for the virus in counteracting host defenses, since Δ2A viruses were no longer pathogenic and were unable to inhibit apoptosis in vitro.

Three-dimensional vision based on a combination of gray-code and phase-shift light projection: analysis and compensation of the systematic errors.

A combination of phase-shift with gray-code light projection into a three-dimensional vision system based on the projection of structured light is presented. The gray-code method is exploited to detect without ambiguity even marked surface discontinuities, whereas the phase-shift technique allows the measurement of fine surface details. The system shows excellent linearity. An overall mean value of the measurement error equal to 40 microm, with a variability of approximately +/-35 microm, corresponding to 0.06% of full scale, has been estimated. The implementation of the technique is discussed, the analysis of the systematic errors is presented in detail, and the calibration procedure designed to determine the optimal setting of the measurement parameters is illustrated.

Low pressure pneumatic tourniquets: effectiveness at minimum recommended inflation pressures.

Complications from lower extremity pneumatic tourniquet use can range from transient to devastating. A major factor that can lead to complications is simply the applied site pressure. Recent advancements in tourniquet design, including increased width and curve allow for consistent hemostasis at pressures as low as 200 mm. Hg for single bladder pneumatic cuffs applied at the ankle, and 250 mm. Hg applied at the thigh. The Zimmer Automatic Tourniquet System (A.T.S.) Low Pressure Cuffs were followed at these minimal pressures with promising results.