Salivary fluoride bioavailability after use of high-fluoride dentifrices with different compositions: A short-term randomized clinical trial.

OBJECTIVE: The objective of the study was to evaluate the kinetics of salivary F bioavailability after the use of high-fluoride dentifrices with different compositions and their amount of total soluble fluoride (TSF). METHODS: A short-term clinical randomized trial was performed in which 15 adult participants were randomly allocated into three groups: 5000 ppm F-dentifrice, 5000 ppm F-dentifrice + TCP (tri-calcium phosphate) and 1450 ppm F-dentifrice. Unstimulated saliva was collected at different times: baseline (before toothbrushing), immediately after brushing/water rinsing and at 5, 15 and 30 min and 1, 2, 4, 8 and 12 h after brushing. The TSF in dentifrices and saliva samples was analysed using an ion-specific electrode. For statistical analysis, the paired t-test and Kruskal-Wallis were used with Dunn's post-test with a 95% confidence interval. RESULTS: There was no significant difference between the declared TSF and that found in 5000 ppm F-dentifrice and 1450 ppm F-dentifrice (p ≥ 0.13); however, in the 5000 ppm F-dentifrice + TCP, approximately 500 ppm less TSF was observed (p = 0.0024). The area under the curve (AUC, µg F/ml min-1 ) of both high-fluoride dentifrices (321.7 ± 84.0 and 223.6 ± 55.1 for the one without and with TCP, respectively) was higher than the conventional one (89.97 ± 15.6) attesting a higher F-bioavailability (p = 0.04). Furthermore, they were able to provide F-salivary levels higher than the baseline for up to 2 h, while this time was 1 h for the 1450 ppm F-dentifrice (p ≤ 0.003). CONCLUSION: Both high-fluoride dentifrices similarly increased the salivary-F bioavailability in comparison with 1450 ppm F-dentifrice, despite the lower TSF presented by the dentifrice containing TCP.

Effect of high-fluoride dentifrice on root dentine and bacterial composition in a multispecies biofilm model.

The present study evaluated the effect of high-fluoride dentifrice on dentine demineralization and bacterial composition in a multispecies biofilm model in vitro. A seven-organism bacterial consortium was grown on bovine dentine discs in a high-throughput active attachment model. The biofilms were submitted twice per day to the following dentifrices treatments: 5,000 ppm F, 1,100 ppm F, with placebo as a negative control. After 5 days of biofilm growth, dentine samples were assessed by transversal microradiography, the biofilm was collected for bacterial counts and the pH of the media was determined. Lower integrated mineral loss values were observed when 5,000 ppm F-treatment was used compared to the other treatments. Overall microbiological counts decreased with increasing F-concentration as well the pH of the media throughout the experiment. The 5,000 ppm F-treatment caused a shift in microbial composition and reduced dentine demineralization in the in-vitro experimental model.

Nocturnal Salivary Fluoride Bioavailability After Brushing with a High-fluoride Dentifrice.

The use of fluoridated dentifrices is recognized as the main reason for the decline of dental caries and its effect is associated with the bioavailability of fluoride (F) in the oral cavity. High-fluoride dentifrice has been indicated for patients at high risk of caries and management of root lesions. This study aimed to evaluate the bioavailability of F in saliva after the use of high-fluoride dentifrice during the nocturnal period. Fifteen healthy adults participated in this is in vivo and crossover study in which the concentration of F in their saliva was determined after brushing with the tested dentifrices: a conventional (1450 ppm F) or with high-fluoride concentration (5000 ppm F). Before brushing, the participants collected the non-stimulated saliva (baseline), immediately after brushing (time zero) and after 5min, 2h, 4h, and 8h, during the nocturnal period (between 10:00 pm and 06:00 am). The salivary F concentration was determined using a specific F ion electrode. Regarding statistical analysis, a paired t-test was used to compare dentifrices with p fixed at 5%. At baseline, there was no significant difference between groups (p>0.001). Immediately after brushing, both dentifrices increased the F salivary concentration, with the highest concentration reached in time zero; however, the use of 5000 ppm F dentifrice maintained the higher F salivary concentration at all times evaluated (p<0.001), remaining higher until 8 h after brushing. Furthermore, this treatment showed higher F bioavailability in relation to time, evaluated by the area under the curve (p<0.001). Thus, it can be concluded that the high-fluoride dentifrice increased the bioavailability of salivary F during the nocturnal period in comparison with conventional dentifrice.

O efeito do tabagismo na ocorrência da cárie dentária: uma revisão de literatura / The effect of smoking on the occurrence of dental caries: a literature review

Introdução: A cárie dentária é a doença bucal mais prevalente no mundo, sendo uma das mais estudadas há anos. Diversos fatores estão envolvidos em seu desenvolvimento, alguns de forma direta, outros indiretamente. O hábito de fumar considerado como contribuinte para diversas patologias bucais, como doença periodontal, câncer oral e atualmente relacionado com a doença cárie. Objetivo: Analisar a associação do tabagismo com a ocorrência da cárie dentária. Materiais e Métodos: Este trabalho tratou-se de um estudo de revisão de literatura sobre os efeitos do tabagismo na cárie dentária onde as informações foram obtidas a partir de 61 artigos científicos em português, inglês, espanhol e francês, consultados em bases de dados online, com disponibilidade na íntegra, que apresentavam coerência com a temática e com data de publicação entre 2008 e 2019. Foram excluídas da pesquisa, publicações cujos títulos e/ou objetivos não possuíam ligação direta com a temática ou que fugiam do objeto de estudo. Resultados: Os resultados da literatura mostraram que fumantes têm risco aumentado de desenvolver cárie dental em comparação com não fumantes. A nicotina é apontada como o componente do tabaco responsável pelo aumento da proliferação bacteriana dentro do biofilme dental. Além disso os biofilmes tornam-se mais compactos e resistentes em presença dessa substância. Conclusão: A comunidade científica tem mostrado que a nicotina é um dos fatores modificadores para o desenvolvimento da doença, sendo necessárias mais trabalhos nesse âmbito para elucidar essa questão.

Introduction: Dental caries is the most prevalent oral disease in the world, being one of the most studied for years. Several factors are involved in its development, some directly, others indirectly. The habit of smoking has been considered as a contributor to several oral pathologies, such as periodontal disease, oral cancer and currently has been related to caries disease. Objective: To analyze the association between smoking and the occurrence of dental caries. Materials and Methods: This work was a study of literature review on the effects of smoking on dental caries where the information was obtained from 61 scientific articles in Portuguese, English, Spanish and French, consulted in online databases , with full availability, which were coherent with the theme and with publication date between 2008 and 2019. Publications whose titles and / or objectives did not have a direct link with the theme or which escaped the object of study were excluded from the research. Results: Results in the literature have shown that smokers are at increased risk of developing dental caries compared to non-smokers. Nicotine is identified as the tobacco component responsible for the increase in bacterial proliferation within the dental biofilm. In addition, biofilms become more compact and resistant in the presence of this substance. Conclusion: The scientific community has shown that nicotine is one of the modifying factors that contributes to the development of the disease, requiring further research in this area to further clarify this issue.